FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_34.34100000007703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_34.34100000007703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365805
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9470.2588810978526811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1200.043.12316543
ACGAGAC1300.039.8114422
CGTATGC1300.039.80641
CTCGTAT1350.038.33170339
TCTCGTA1350.038.33170338
TCGTATG1350.038.33170340
TGCCGTC1350.038.33170345
CACGAGA1400.036.96776621
ATCTCGT1400.036.9627137
GATCTCG1350.036.66510836
TCTCCGA1450.035.6930211
ATCTCCG1500.034.5032510
CCCACGA1550.033.38567719
CCACGAG1600.032.34679420
ATGCCGT1600.032.34237344
GCCCACG1650.031.36230318
CGAGACA1650.030.00282523
CTCCGAG1850.029.1919412
CGAGCCC1800.028.75270715
GTATGCC1850.027.97178542
Produced by FastQC (version 0.11.2)