FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_33.341000000076e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_33.341000000076e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223294
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC5540.24810339731475098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAT207.025541E-444.994184
CTCGTAT850.042.3474639
ATCTCCG800.042.18204510
CGTATGC950.040.2579541
CACGAGA900.039.99482321
ACGAGAC950.037.88983522
TAATATG250.00210481835.9953425
TAGACCC250.00210481835.9953424
TATGCCG1000.035.99534243
TCTCGTA1000.035.99534238
TCGTATG1000.035.99534240
GCTAATA250.00210481835.9953423
CCCACGA1100.034.76822719
TGCCGTC1050.034.28895645
GTATGCC1150.033.25656542
GATGTGA1100.030.67784931
ATGTGAT1100.030.67784932
TCTCCGA1100.030.67784911
CGAGCCC1200.030.0095615
AATATGC300.00514173529.9961176
Produced by FastQC (version 0.11.2)