FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_31.341000000076ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_31.341000000076ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC271.7555266579973992No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC181.1703511053315996No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC171.1053315994798438RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC140.9102730819245773No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC110.7152145643693107No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC90.5851755526657998No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC90.5851755526657998RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC70.45513654096228867No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC70.45513654096228867RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC60.3901170351105332No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC50.3250975292587776No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC50.3250975292587776No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC50.3250975292587776No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.26007802340702213No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC40.26007802340702213No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC40.26007802340702213No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC20.13003901170351106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13003901170351106No Hit
GTGCCTGGGCCTTCATGATCCGTTCCATGTTGGCTGTCCAGCCATAGGTGC20.13003901170351106No Hit
CTCGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGC20.13003901170351106No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC20.13003901170351106No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC20.13003901170351106No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)