FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_30.341000000076b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_30.341000000076b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC493.099304237824162No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC231.454775458570525RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC110.6957621758380772RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT70.4427577482605946No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC60.3795066413662239No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC50.31625553447185323No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.31625553447185323RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC50.31625553447185323No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC40.2530044275774826No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.18975332068311196No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.18975332068311196No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT30.18975332068311196No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC30.18975332068311196No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC30.18975332068311196No Hit
CTTATACACATCTTCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC30.18975332068311196No Hit
CTCCTTCACCTTTTTGGTGAGGTTTATTCCTAGGTATCTTACGCCTGTCTC20.1265022137887413No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT20.1265022137887413No Hit
CAACTCCATTTGGTAACAACGGTGAAATCGTTGAAAAATATCGTATTACGA20.1265022137887413No Hit
AACAATCTGCCAATAAATGCACAAAGAGAAATGGATGATGGGAACCTGTCT20.1265022137887413No Hit
CATCATGAACATGCAGAGAGGACATCTTATGAAACAACTTAAACCTGTCTC20.1265022137887413No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTATG20.1265022137887413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC252.36841E-545.022
AGCCCAC301.0716958E-644.99999617
ATCTCCG301.0716958E-644.99999610
GCCCACG301.0716958E-644.99999618
TATACAC301.0716958E-644.9999963
CCACGAG301.0716958E-644.99999620
GAGCCCA301.0716958E-644.99999616
CACGAGA301.0716958E-644.99999621
CCCACGA301.0716958E-644.99999619
TTATACA301.0716958E-644.9999962
CGAGCCC301.0716958E-644.99999615
CCGAGCC301.0716958E-644.99999614
TCTCCGA301.0716958E-644.99999611
TCCGAGC301.0716958E-644.99999613
ATACACA301.0716958E-644.9999964
CTCCGAG301.0716958E-644.99999612
CACATCT353.1055934E-638.5714267
CATCTCC353.1055934E-638.5714269
TACACAT353.1055934E-638.5714265
ACACATC353.1055934E-638.5714266
Produced by FastQC (version 0.11.2)