##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 0h_3.341000000074dd.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3899
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.572197999487045	38.0	32.0	38.0	32.0	38.0
2	36.0492433957425	38.0	38.0	38.0	32.0	38.0
3	35.89048473967684	38.0	32.0	38.0	32.0	38.0
4	36.24724288279046	38.0	38.0	38.0	32.0	38.0
5	36.312644267760966	38.0	38.0	38.0	32.0	38.0
6	38.360092331367014	40.0	38.0	40.0	38.0	40.0
7	37.75327006924852	40.0	38.0	40.0	32.0	40.0
8	37.767376250320595	40.0	38.0	40.0	32.0	40.0
9	38.148756091305465	40.0	38.0	40.0	38.0	40.0
10	37.94588355988715	40.0	38.0	40.0	32.0	40.0
11	38.1410618107207	40.0	38.0	40.0	38.0	40.0
12	37.58886894075404	38.0	38.0	40.0	32.0	40.0
13	37.921774814054885	40.0	38.0	40.0	32.0	40.0
14	37.530905360348804	40.0	38.0	40.0	32.0	40.0
15	37.723775327006926	40.0	38.0	40.0	32.0	40.0
16	36.95537317260836	38.0	38.0	40.0	32.0	40.0
17	36.908438061041295	38.0	38.0	40.0	32.0	40.0
18	36.67478840728392	38.0	38.0	40.0	32.0	40.0
19	37.14311361887663	38.0	38.0	40.0	32.0	40.0
20	37.331367017183894	40.0	38.0	40.0	32.0	40.0
21	37.39702487817389	40.0	38.0	40.0	32.0	40.0
22	37.67248012310849	40.0	38.0	40.0	32.0	40.0
23	37.63965119261349	40.0	38.0	40.0	32.0	40.0
24	37.269299820466784	40.0	38.0	40.0	32.0	40.0
25	36.85098743267505	38.0	38.0	40.0	32.0	40.0
26	36.72197999487048	38.0	38.0	40.0	32.0	40.0
27	36.652218517568606	38.0	38.0	40.0	32.0	40.0
28	36.74865350089767	38.0	38.0	40.0	32.0	40.0
29	36.968453449602464	38.0	38.0	40.0	32.0	40.0
30	37.415234675557834	40.0	38.0	40.0	32.0	40.0
31	37.451654270325726	38.0	38.0	40.0	32.0	40.0
32	37.48371377276224	40.0	38.0	40.0	32.0	40.0
33	36.99794819184406	38.0	38.0	40.0	32.0	40.0
34	36.89817902026161	38.0	38.0	40.0	32.0	40.0
35	37.36240061554245	38.0	38.0	40.0	32.0	40.0
36	37.563734290843804	38.0	38.0	40.0	32.0	40.0
37	36.89920492433957	38.0	38.0	40.0	32.0	40.0
38	37.10977173634265	38.0	38.0	40.0	32.0	40.0
39	36.46191331110541	38.0	38.0	40.0	32.0	40.0
40	36.74121569633239	38.0	38.0	40.0	32.0	40.0
41	36.836881251602975	38.0	38.0	40.0	32.0	40.0
42	36.9310079507566	38.0	38.0	40.0	32.0	40.0
43	37.47165939984612	38.0	38.0	40.0	32.0	40.0
44	37.18184149781995	38.0	38.0	40.0	32.0	40.0
45	37.26801744036933	38.0	38.0	40.0	32.0	40.0
46	36.6588868940754	38.0	38.0	40.0	32.0	40.0
47	36.72351885098743	38.0	38.0	40.0	32.0	40.0
48	36.76199025391126	38.0	38.0	40.0	32.0	40.0
49	36.398563734290846	38.0	38.0	40.0	27.0	40.0
50	36.33264939728135	38.0	38.0	40.0	27.0	40.0
51	35.790202616055396	38.0	38.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	2.3912388392857125
2201	2	0.68242187500001
2201	3	0.8315104166666742
2201	4	0.3312686011904802
2201	5	1.7676339285714278
2201	6	0.371763392857126
2201	7	0.8993861607142719
2201	8	1.1462239583333513
2201	9	1.147953869047626
2201	10	1.246595982142864
2201	11	0.5075892857142961
2201	12	1.6366257440476133
2201	13	0.520554315476204
2201	14	1.6966703869047706
2201	15	1.4059895833333371
2201	16	2.11378348214285
2201	17	1.5835751488095369
2201	18	2.017912946428588
2201	19	2.1417410714285623
2201	20	2.414490327380946
2201	21	2.054445684523813
2201	22	2.253162202380956
2201	23	1.914043898809524
2201	24	2.1099702380952436
2201	25	2.293898809523796
2201	26	2.6391927083333186
2201	27	2.63450520833333
2201	28	2.5702008928571516
2201	29	1.727027529761891
2201	30	1.2265252976190553
2201	31	1.5716703869047635
2201	32	1.6840959821428712
2201	33	1.5114769345238201
2201	34	1.6681547619047592
2201	35	0.18524925595238528
2201	36	1.5293526785714349
2201	37	1.760323660714306
2201	38	1.5891741071428669
2201	39	2.9515811011904844
2201	40	2.5824404761904844
2201	41	2.813764880952391
2201	42	2.607440476190483
2201	43	2.0008184523809547
2201	44	2.1094308035714278
2201	45	1.7741071428571544
2201	46	1.6032180059523853
2201	47	2.2990141369047663
2201	48	1.7423549107142904
2201	49	2.5821056547619037
2201	50	2.934319196428582
2201	51	3.2638578869047663
1101	1	1.1912388392857096
1101	2	0.4824218750000071
1101	3	0.6315104166666714
1101	4	0.13126860119047734
1101	5	-0.6323660714285708
1101	6	0.5717633928571288
1101	7	-0.10061383928572809
1101	8	1.0128906250000185
1101	9	-0.5853794642857082
1101	10	0.3799293154761969
1101	11	-0.8924107142857025
1101	12	0.7699590773809462
1101	13	0.6538876488095369
1101	14	1.5633370535714377
1101	15	1.2726562500000043
1101	16	-0.21954985119048587
1101	17	0.9169084821428726
1101	18	0.6179129464285893
1101	19	1.2750744047618952
1101	20	1.2144903273809433
1101	21	1.2544456845238159
1101	22	1.0531622023809533
1101	23	1.047377232142857
1101	24	0.04330357142858077
1101	25	0.9605654761904603
1101	26	0.6391927083333186
1101	27	1.7678385416666629
1101	28	2.10353422619049
1101	29	1.9270275297618937
1101	30	1.0265252976190524
1101	31	1.4383370535714306
1101	32	1.6174293154762083
1101	33	1.711476934523823
1101	34	1.934821428571425
1101	35	0.18524925595238528
1101	36	-0.603980654761898
1101	37	0.36032366071430744
1101	38	0.5891741071428669
1101	39	2.0849144345238173
1101	40	1.3157738095238187
1101	41	2.013764880952394
1101	42	1.4074404761904802
1101	43	2.0008184523809547
1101	44	2.042764136904765
1101	45	0.9074404761904873
1101	46	0.2032180059523867
1101	47	0.23234747023810343
1101	48	0.6756882440476275
1101	49	2.048772321428565
1101	50	1.200985863095248
1101	51	1.530524553571432
2202	1	1.3912388392857125
2202	2	1.68242187500001
2202	3	1.8315104166666742
2202	4	-0.6687313988095198
2202	5	0.7676339285714278
2202	6	0.038430059523790305
2202	7	1.5660528273809362
2202	8	0.14622395833335133
2202	9	0.14795386904762609
2202	10	1.246595982142864
2202	11	1.1742559523809604
2202	12	1.3032924107142847
2202	13	1.520554315476204
2202	14	1.6966703869047706
2202	15	2.0726562500000014
2202	16	2.780450148809514
2202	17	2.583575148809537
2202	18	2.684579613095252
2202	19	1.8084077380952337
2202	20	2.0811569940476105
2202	21	-0.11222098214285126
2202	22	1.9198288690476204
2202	23	1.5807105654761955
2202	24	0.776636904761915
2202	25	2.6272321428571246
2202	26	1.9725260416666544
2202	27	2.3011718750000014
2202	28	1.236867559523823
2202	29	1.0603608630952266
2202	30	1.559858630952391
2202	31	1.5716703869047635
2202	32	1.6840959821428712
2202	33	1.5114769345238201
2202	34	1.6681547619047592
2202	35	0.518582589285721
2202	36	1.5293526785714349
2202	37	1.0936569940476417
2202	38	2.255840773809531
2202	39	2.618247767857156
2202	40	1.5824404761904844
2202	41	2.1470982142857267
2202	42	0.9407738095238187
2202	43	1.667485119047619
2202	44	1.4427641369047635
2202	45	1.7741071428571544
2202	46	1.6032180059523853
2202	47	2.2990141369047663
2202	48	1.7423549107142904
2202	49	2.248772321428568
2202	50	1.6009858630952465
2202	51	2.9305245535714306
1102	1	0.14123883928571246
1102	2	0.93242187500001
1102	3	0.33151041666667425
1102	4	0.5812686011904802
1102	5	0.26763392857142776
1102	6	1.121763392857126
1102	7	1.399386160714272
1102	8	1.3128906250000156
1102	9	0.3146205357142904
1102	10	0.32992931547619975
1102	11	0.5075892857142961
1102	12	1.469959077380949
1102	13	1.1038876488095397
1102	14	1.3633370535714349
1102	15	1.3226562500000014
1102	16	-1.0945498511904859
1102	17	1.0419084821428726
1102	18	0.5179129464285879
1102	19	1.975074404761898
1102	20	1.6644903273809462
1102	21	1.804445684523813
1102	22	1.503162202380956
1102	23	0.7473772321428598
1102	24	0.6933035714285793
1102	25	1.7105654761904603
1102	26	2.1391927083333186
1102	27	-0.6571614583333343
1102	28	0.02853422619048729
1102	29	0.4770275297618909
1102	30	0.35152529761905527
1102	31	0.9883370535714278
1102	32	1.5174293154762069
1102	33	2.01147693452382
1102	34	1.8348214285714235
1102	35	1.6852492559523853
1102	36	1.1960193452380992
1102	37	1.760323660714306
1102	38	0.8391741071428669
1102	39	-1.0900855654761799
1102	40	-4.08422619047618
1102	41	-3.186235119047609
1102	42	-4.392559523809517
1102	43	-0.12418154761904532
1102	44	1.4427641369047635
1102	45	-2.76755952380951
1102	46	0.4782180059523853
1102	47	1.882347470238102
1102	48	2.075688244047626
1102	49	2.248772321428568
1102	50	1.6009858630952465
1102	51	2.4305245535714306
2203	1	1.3912388392857125
2203	2	-0.31757812499999005
2203	3	0.8315104166666742
2203	4	0.3312686011904802
2203	5	1.7676339285714278
2203	6	0.7050967261904617
2203	7	0.2327194940476076
2203	8	1.1462239583333513
2203	9	1.8146205357142904
2203	10	1.246595982142864
2203	11	0.8409226190476318
2203	12	-3.1967075892857153
2203	13	0.18722098214287541
2203	14	-0.46999627976190084
2203	15	1.0726562500000014
2203	16	2.4471168154761784
2203	17	2.2502418154762083
2203	18	3.017912946428588
2203	19	2.1417410714285623
2203	20	2.0811569940476105
2203	21	0.38777901785714874
2203	22	0.0864955357142918
2203	23	0.24737723214285978
2203	24	-2.0566964285714207
2203	25	1.6272321428571246
2203	26	-1.8608072916666814
2203	27	0.9678385416666657
2203	28	0.736867559523823
2203	29	0.5603608630952266
2203	30	-2.940141369047609
2203	31	0.07167038690476346
2203	32	1.3507626488095426
2203	33	0.8448102678571558
2203	34	2.001488095238088
2203	35	1.8519159226190496
2203	36	1.1960193452380992
2203	37	1.760323660714306
2203	38	1.5891741071428669
2203	39	2.9515811011904844
2203	40	2.249107142857156
2203	41	2.1470982142857267
2203	42	0.9407738095238187
2203	43	1.3341517857142904
2203	44	1.1094308035714278
2203	45	1.1074404761904901
2203	46	1.2698846726190496
2203	47	1.9656808035714377
2203	48	-0.09097842261903821
2203	49	1.248772321428568
2203	50	2.934319196428582
2203	51	1.097191220238095
1103	1	1.1912388392857096
1103	2	0.4824218750000071
1103	3	1.8315104166666742
1103	4	1.3312686011904802
1103	5	0.5676339285714249
1103	6	0.9717633928571274
1103	7	1.0993861607142748
1103	8	1.0128906250000185
1103	9	1.4146205357142918
1103	10	-4.220070684523797
1103	11	0.7075892857142989
1103	12	1.1699590773809518
1103	13	1.4538876488095411
1103	14	1.9633370535714363
1103	15	1.6726562500000028
1103	16	0.7804501488095141
1103	17	0.9169084821428726
1103	18	0.017912946428587873
1103	19	-3.324925595238099
1103	20	0.41449032738094616
1103	21	1.6544456845238145
1103	22	-0.14683779761904248
1103	23	1.4473772321428626
1103	24	1.2433035714285765
1103	25	2.160565476190463
1103	26	1.8391927083333215
1103	27	2.567838541666667
1103	28	2.10353422619049
1103	29	1.9270275297618937
1103	30	-0.7734747023809447
1103	31	-0.9616629464285751
1103	32	1.2174293154762097
1103	33	1.711476934523823
1103	34	1.1348214285714207
1103	35	1.7852492559523867
1103	36	1.396019345238102
1103	37	1.5603236607143032
1103	38	0.5891741071428669
1103	39	1.2849144345238201
1103	40	2.115773809523823
1103	41	2.4137648809523924
1103	42	1.8074404761904859
1103	43	1.600818452380956
1103	44	-3.3572358630952337
1103	45	-0.8925595238095099
1103	46	1.4032180059523824
1103	47	0.632347470238102
1103	48	2.275688244047629
1103	49	2.048772321428565
1103	50	2.4009858630952436
1103	51	2.7305245535714278
2204	1	-5.775427827380955
2204	2	-0.31757812499999005
2204	3	-3.1684895833333258
2204	4	-0.6687313988095198
2204	5	-2.2323660714285722
2204	6	0.038430059523790305
2204	7	-3.2672805059523924
2204	8	-0.8537760416666487
2204	9	-1.5187127976190453
2204	10	-2.253404017857136
2204	11	-1.6590773809523682
2204	12	-0.6967075892857153
2204	13	0.18722098214287541
2204	14	-1.3033296130952294
2204	15	-0.5940104166666629
2204	16	-1.2195498511904859
2204	17	-0.5830915178571274
2204	18	-2.482087053571412
2204	19	0.475074404761898
2204	20	-2.585509672619054
2204	21	-0.27888764880952266
2204	22	0.5864955357142918
2204	23	0.5807105654761955
2204	24	-1.8900297619047564
2204	25	-4.206101190476204
2204	26	-0.5274739583333456
2204	27	-5.032161458333334
2204	28	-0.763132440476177
2204	29	-4.106305803571445
2204	30	-1.940141369047609
2204	31	-0.09499627976190794
2204	32	-0.31590401785712885
2204	33	-0.15518973214284415
2204	34	0.6681547619047592
2204	35	-5.981417410714283
2204	36	-0.8039806547619008
2204	37	-3.4063430059523583
2204	38	-3.244159226190469
2204	39	-3.881752232142844
2204	40	-5.250892857142848
2204	41	-1.686235119047609
2204	42	-1.392559523809517
2204	43	-0.9991815476190453
2204	44	-3.723902529761901
2204	45	-1.8925595238095099
2204	46	-2.8967819940476147
2204	47	-0.867652529761898
2204	48	0.07568824404762609
2204	49	-2.4178943452380963
2204	50	-2.232347470238089
2204	51	-3.2361421130952337
2205	1	-2.775427827380952
2205	2	-3.4842447916666544
2205	3	-1.0018229166666615
2205	4	1.3312686011904802
2205	5	-0.23236607142857224
2205	6	-0.2949032738095383
2205	7	0.2327194940476076
2205	8	1.1462239583333513
2205	9	0.14795386904762609
2205	10	0.9132626488095354
2205	11	1.1742559523809604
2205	12	0.969959077380949
2205	13	1.1872209821428754
2205	14	0.6966703869047706
2205	15	-3.094010416666663
2205	16	-2.38621651785715
2205	17	-0.41642485119046313
2205	18	-3.1487537202380764
2205	19	0.8084077380952337
2205	20	-1.4188430059523895
2205	21	-2.7788876488095227
2205	22	0.2531622023809561
2205	23	1.914043898809524
2205	24	0.44330357142857935
2205	25	0.6272321428571246
2205	26	-0.19414062500001705
2205	27	1.63450520833333
2205	28	1.9035342261904873
2205	29	0.2270275297618909
2205	30	-2.940141369047609
2205	31	1.2383370535714278
2205	32	1.3507626488095426
2205	33	1.8448102678571558
2205	34	1.6681547619047592
2205	35	1.518582589285721
2205	36	0.8626860119047635
2205	37	1.4269903273809703
2205	38	-0.24415922619046881
2205	39	-1.5484188988095156
2205	40	-2.250892857142844
2205	41	0.813764880952391
2205	42	-1.5592261904761813
2205	43	8.184523809546818E-4
2205	44	1.7760974702380992
2205	45	1.1074404761904901
2205	46	1.936551339285721
2205	47	0.632347470238102
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1211	26	-2.0750930059523967
1211	27	-1.0321614583333343
1211	28	-5.95360863095237
1211	29	-1.415829613095255
1211	30	-1.2020461309523753
1211	31	-0.33309151785714164
1211	32	-1.125427827380939
1211	33	-0.6313802083333258
1211	34	-5.379464285714292
1211	35	-0.3861793154761841
1211	36	-1.8039806547619008
1211	37	-0.6682477678571246
1211	38	1.1606026785714363
1211	39	0.9992001488095354
1211	40	0.6300595238095354
1211	41	-0.9005208333333243
1211	42	-0.5354166666666629
1211	43	-0.28489583333333
1211	44	0.44276413690476346
1211	45	-1.0354166666666558
1211	46	-1.6824962797618994
1211	47	0.9180617559523867
1211	48	1.6471168154761955
1211	49	-1.1797991071428626
1211	50	-0.8275855654761841
1211	51	1.9305245535714306
1208	1	1.3912388392857125
1208	2	0.68242187500001
1208	3	-0.16848958333332575
1208	4	-0.6687313988095198
1208	5	0.7676339285714278
1208	6	0.371763392857126
1208	7	-2.7672805059523924
1208	8	-2.3537760416666487
1208	9	0.14795386904762609
1208	10	0.9132626488095354
1208	11	-1.1590773809523682
1208	12	0.3032924107142847
1208	13	-0.479445684523796
1208	14	1.3633370535714349
1208	15	1.0726562500000014
1208	16	-2.38621651785715
1208	17	0.5835751488095369
1208	18	0.6845796130952522
1208	19	-3.6915922619047663
1208	20	-2.085509672619054
1208	21	-1.6122209821428513
1208	22	-0.4135044642857082
1208	23	-0.7526227678571402
1208	24	0.10997023809524364
1208	25	-0.206101190476204
1208	26	-0.02747395833334565
1208	27	-6.365494791666666
1208	28	-3.263132440476177
1208	29	0.06036086309522659
1208	30	-3.2734747023809447
1208	31	-1.9283296130952365
1208	32	0.6840959821428712
1208	33	0.17814360119048445
1208	34	0.6681547619047592
1208	35	-0.481417410714279
1208	36	-0.13731398809523654
1208	37	0.4269903273809703
1208	38	-1.0774925595237974
1208	39	-3.21508556547618
1208	40	-0.41755952380951555
1208	41	-4.186235119047609
1208	42	1.607440476190483
1208	43	8.184523809546818E-4
1208	44	1.1094308035714278
1208	45	-0.22589285714284557
1208	46	-4.23011532738095
1208	47	-3.2009858630952337
1208	48	-4.25764508928571
1208	49	-4.084561011904768
1208	50	-1.8990141369047535
1208	51	-3.7361421130952337
1209	1	-0.7337611607142875
1209	2	-0.56757812499999
1209	3	-2.0434895833333258
1209	4	-0.16873139880951982
1209	5	0.26763392857142776
1209	6	-0.378236607142874
1209	7	0.8993861607142719
1209	8	-0.18710937499998437
1209	9	-0.6853794642857096
1209	10	0.5799293154761997
1209	11	-0.49241071428570393
1209	12	-2.905040922619051
1209	13	-3.0211123511904603
1209	14	-2.136662946428565
1209	15	-0.4273437499999986
1209	16	-4.469549851190486
1209	17	-2.3330915178571274
1209	18	-3.232087053571412
1209	19	-5.149925595238102
1209	20	-8.210509672619054
1209	21	-1.070554315476187
1209	22	-2.621837797619044
1209	23	-0.2526227678571402
1209	24	1.4433035714285793
1209	25	-1.4144345238095397
1209	26	-3.1108072916666814
1209	27	-1.6571614583333343
1209	28	-5.471465773809513
1209	29	-1.897972470238109
1209	30	-1.8984747023809447
1209	31	-3.3866629464285722
1209	32	-0.35757068452379315
1209	33	-0.6135230654761799
1209	34	-4.2901785714285765
1209	35	-1.9397507440476147
1209	36	-0.17898065476190084
1209	37	-1.239676339285694
1209	38	-1.7858258928571331
1209	39	-0.9650855654761799
1209	40	-1.4592261904761799
1209	41	-2.061235119047609
1209	42	-2.267559523809517
1209	43	-3.8741815476190453
1209	44	-3.8072358630952365
1209	45	-2.01755952380951
1209	46	-3.0217819940476147
1209	47	-1.992652529761898
1209	48	0.20068824404762609
1209	49	-1.626227678571432
1209	50	-0.5240141369047535
1209	51	-3.3194754464285694
1214	1	2.3912388392857125
1214	2	-0.03186383928570535
1214	3	1.8315104166666742
1214	4	-0.3830171130952351
1214	5	0.9104910714285737
1214	6	0.8003348214285566
1214	7	1.3279575892857025
1214	8	0.38431919642858503
1214	9	0.10033482142857508
1214	10	-0.13435639880951555
1214	11	0.9361607142857267
1214	12	0.1128162202380949
1214	13	0.7110305059523938
1214	14	-5.35094866071428
1214	15	-0.9273437499999986
1214	16	-0.7909784226190553
1214	17	1.2026227678571573
1214	18	1.3036272321428726
1214	19	1.617931547619044
1214	20	-2.299795386904769
1214	21	-0.802697172619041
1214	22	0.5388764880952408
1214	23	0.8188058035714292
1214	24	0.729017857142864
1214	25	2.103422619047606
1214	26	1.210621279761888
1214	27	0.8249813988095198
1214	28	0.7606770833333414
1214	29	-2.558686755952394
1214	30	-3.0591889880952294
1214	31	0.09547991071428186
1214	32	-2.6968563988095084
1214	33	-2.48852306547618
1214	34	-0.6651785714285765
1214	35	-2.52903645833333
1214	36	-5.518266369047616
1214	37	0.3317522321428754
1214	38	0.8748883928571516
1214	39	2.1420572916666742
1214	40	1.7729166666666742
1214	41	1.9566220238095369
1214	42	1.4645833333333371
1214	43	1.4293898809523853
1214	44	1.871335565476194
1214	45	2.10744047619049
1214	46	1.7460751488095312
1214	47	0.9180617559523867
1214	48	1.9328311011904802
1214	49	1.5344866071428527
1214	50	-1.1132998511904688
1214	51	2.501953125
1215	1	2.3912388392857125
1215	2	1.68242187500001
1215	3	-0.5684895833333243
1215	4	0.13126860119047734
1215	5	0.5676339285714249
1215	6	0.17176339285712316
1215	7	0.6993861607142691
1215	8	-1.1871093749999844
1215	9	0.6146205357142875
1215	10	0.7799293154762026
1215	11	1.1075892857142975
1215	12	1.1699590773809518
1215	13	-3.9461123511904574
1215	14	1.5633370535714377
1215	15	1.2726562500000043
1215	16	1.1804501488095127
1215	17	-0.08309151785712743
1215	18	2.6179129464285893
1215	19	2.0750744047618994
1215	20	0.41449032738094616
1215	21	-0.34555431547618554
1215	22	1.0531622023809533
1215	23	-3.952622767857143
1215	24	0.44330357142857935
1215	25	0.9605654761904603
1215	26	0.6391927083333186
1215	27	2.567838541666667
1215	28	-2.89646577380951
1215	29	-0.6729724702381077
1215	30	1.0265252976190524
1215	31	1.4383370535714306
1215	32	0.817429315476204
1215	33	0.5114769345238201
1215	34	0.3348214285714235
1215	35	1.3852492559523881
1215	36	1.7960193452381006
1215	37	2.3603236607143074
1215	38	1.7891741071428697
1215	39	2.484914434523823
1215	40	0.5157738095238216
1215	41	2.013764880952394
1215	42	0.607440476190483
1215	43	1.2008184523809575
1215	44	1.6427641369047663
1215	45	1.7074404761904916
1215	46	1.8032180059523881
1215	47	1.4323474702380992
1215	48	0.6756882440476275
1215	49	1.248772321428568
1215	50	0.2009858630952479
1215	51	-1.6694754464285708
1212	1	1.1912388392857096
1212	2	1.68242187500001
1212	3	1.8315104166666742
1212	4	1.3312686011904802
1212	5	0.5676339285714249
1212	6	-5.228236607142875
1212	7	0.6993861607142691
1212	8	-3.9871093749999815
1212	9	-1.1853794642857096
1212	10	-0.42007068452380025
1212	11	-0.8924107142857025
1212	12	-1.8300409226190482
1212	13	-2.546112351190459
1212	14	-9.236662946428567
1212	15	-1.7273437499999957
1212	16	-2.219549851190486
1212	17	-0.28309151785713027
1212	18	-0.9820870535714121
1212	19	-2.524925595238102
1212	20	0.014490327380947576
1212	21	1.2544456845238159
1212	22	1.453162202380959
1212	23	1.047377232142857
1212	24	1.2433035714285765
1212	25	-3.639434523809541
1212	26	-0.5608072916666842
1212	27	-0.23216145833333712
1212	28	0.9035342261904873
1212	29	-4.272972470238109
1212	30	1.0265252976190524
1212	31	-3.961662946428575
1212	32	-1.5825706845237946
1212	33	-3.0885230654761813
1212	34	-4.265178571428578
1212	35	0.18524925595238528
1212	36	0.9960193452380963
1212	37	-0.4396763392856968
1212	38	0.5891741071428669
1212	39	1.6849144345238187
1212	40	-1.0842261904761799
1212	41	0.4137648809523924
1212	42	-3.9925595238095184
1212	43	0.40081845238095326
1212	44	-4.157235863095238
1212	45	-1.2925595238095084
1212	46	1.4032180059523824
1212	47	-5.567652529761897
1212	48	-3.1243117559523768
1212	49	-2.951227678571435
1212	50	-0.3990141369047535
1212	51	-1.0694754464285694
1213	1	1.1912388392857096
1213	2	1.68242187500001
1213	3	-0.5684895833333243
1213	4	-1.0687313988095184
1213	5	-3.0323660714285694
1213	6	0.17176339285712316
1213	7	-0.5006138392857267
1213	8	1.0128906250000185
1213	9	1.0146205357142932
1213	10	0.7799293154762026
1213	11	1.1075892857142975
1213	12	0.7699590773809462
1213	13	-1.7461123511904617
1213	14	1.163337053571432
1213	15	1.2726562500000043
1213	16	1.980450148809517
1213	17	0.516908482142874
1213	18	-0.7820870535714093
1213	19	0.07507440476189942
1213	20	0.41449032738094616
1213	21	1.6544456845238145
1213	22	0.2531622023809561
1213	23	1.4473772321428626
1213	24	1.6433035714285822
1213	25	-3.639434523809541
1213	26	1.4391927083333158
1213	27	-3.232161458333337
1213	28	0.10353422619049013
1213	29	0.3270275297618923
1213	30	1.426525297619058
1213	31	-0.16166294642857082
1213	32	-0.3825706845237917
1213	33	-4.888523065476178
1213	34	-3.4651785714285737
1213	35	0.18524925595238528
1213	36	0.9960193452380963
1213	37	0.36032366071430744
1213	38	-3.610825892857136
1213	39	0.484914434523823
1213	40	-1.0842261904761799
1213	41	1.6137648809523881
1213	42	-3.192559523809514
1213	43	-2.5991815476190467
1213	44	-3.3572358630952337
1213	45	0.10744047619049013
1213	46	-4.796781994047613
1213	47	-0.5676525297619008
1213	48	0.6756882440476275
1213	49	2.048772321428565
1213	50	-2.999014136904755
1213	51	2.330524553571429
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	4.0
24	10.0
25	14.0
26	27.0
27	35.0
28	51.0
29	69.0
30	81.0
31	84.0
32	129.0
33	138.0
34	178.0
35	217.0
36	280.0
37	381.0
38	643.0
39	1557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.874070274429341	3.8984354962810976	9.079251090023083	78.14824313926648
2	4.360092331367017	5.283406001538856	83.48294434470377	6.873557322390357
3	5.1551679917927675	10.464221595280842	77.04539625545011	7.335214157476276
4	3.9753783021287505	74.32675044883304	13.490638625288536	8.207232623749679
5	4.539625545011542	14.567837907155681	77.12233906129778	3.7701974865350087
6	7.5147473711208	79.43062323672737	4.590920748909976	8.463708643241857
7	6.206719671710695	13.4393434213901	6.463195691202872	73.89074121569634
8	5.8733008463708645	78.53295716850475	5.334701205437291	10.2590407796871
9	5.8733008463708645	9.387022313413695	5.898948448320082	78.84072839189535
10	4.796101564503719	76.55809181841498	12.721210566812003	5.9245960502693
11	8.284175429597333	6.078481661964606	75.04488330341114	10.59245960502693
12	5.46293921518338	7.0530905360348815	12.567324955116696	74.91664529366504
13	6.437548089253655	5.1551679917927675	76.22467299307515	12.18261092587843
14	5.206463195691203	6.334957681456784	6.847909720441139	81.61066940241088
15	10.669402410874582	8.309823031546552	6.309310079507566	74.71146447807129
16	75.58348294434471	11.926134906386253	5.5142344190818156	6.976147730187228
17	10.848935624519108	76.40420620671968	6.694024108745832	6.052834060015389
18	76.04513977943063	6.668376506796614	6.873557322390357	10.412926391382406
19	6.078481661964606	8.258527827648116	6.360605283406001	79.30238522698127
20	6.206719671710695	6.360605283406001	6.565786098999743	80.86688894588356
21	5.616824826878688	12.208258527827647	6.129776865863041	76.04513977943063
22	6.71967171069505	74.89099769171582	6.104129263913824	12.285201333675301
23	10.002564760194922	7.873813798409849	6.334957681456784	75.78866375993844
24	75.19876891510644	11.644011284944858	7.1813285457809695	5.975891254167736
25	11.310592459605028	75.76301615798923	6.642728904847396	6.283662477558348
26	75.04488330341114	12.490382149269044	6.386252885355219	6.078481661964606
27	5.9245960502693	76.35291100282123	6.617081302898178	11.105411644011284
28	5.180815593741985	7.848166196460631	6.899204924339575	80.07181328545781
29	5.360348807386509	7.796870992562195	6.873557322390357	79.96922287766094
30	6.309310079507566	7.591690176968453	11.233649653757375	74.86535008976661
31	5.898948448320082	11.72095409079251	74.78840728391896	7.591690176968453
32	10.6950500128238	75.83995896383688	7.386509361374712	6.078481661964606
33	73.07001795332137	12.721210566812003	8.07899461400359	6.129776865863041
34	6.514490895101307	79.71274685816876	7.566042575019235	6.206719671710695
35	6.386252885355219	75.04488330341114	12.490382149269044	6.078481661964606
36	5.360348807386509	12.105668120030778	75.07053090536034	7.463452167222365
37	5.565529622980251	74.96794049756348	12.618620159015132	6.847909720441139
38	6.104129263913824	7.1813285457809695	75.35265452680174	11.361887663503463
39	5.385996409335727	7.001795332136446	12.798153372659657	74.81405488586817
40	6.309310079507566	5.411644011284944	76.22467299307515	12.054372916132342
41	9.976917158245705	6.540138497050525	7.976404206206719	75.50654013849704
42	73.8650936137471	5.9245960502693	12.926391382405743	7.283918953577841
43	5.488586817132598	10.6950500128238	76.7119774301103	7.104385739933317
44	5.411644011284944	75.45524493459862	12.156963323929212	6.976147730187228
45	9.89997435239805	7.386509361374712	76.12208258527828	6.5914337009489605
46	74.01897922544242	6.796614516542704	7.5147473711208	11.669658886894076
47	5.847653244421647	6.668376506796614	7.719928186714542	79.7640420620672
48	10.412926391382406	6.796614516542704	6.95050012823801	75.83995896383688
49	73.1213131572198	6.25801487560913	12.259553731726083	8.361118235444987
50	4.898691972300591	6.206719671710695	76.04513977943063	12.849448576558093
51	4.847396768402154	5.745062836624776	12.721210566812003	76.68632982816106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	1.5
16	3.0
17	2.5
18	2.0
19	1.0
20	0.0
21	1.5
22	3.0
23	2.0
24	1.0
25	2.0
26	2.5
27	2.0
28	5.0
29	8.0
30	13.0
31	18.0
32	18.5
33	19.0
34	22.5
35	26.0
36	40.5
37	55.0
38	58.0
39	61.0
40	72.0
41	83.0
42	92.0
43	101.0
44	86.0
45	71.0
46	81.5
47	92.0
48	223.0
49	354.0
50	1536.5
51	2719.0
52	1399.5
53	80.0
54	66.0
55	52.0
56	42.0
57	32.0
58	27.0
59	22.0
60	21.0
61	20.0
62	18.5
63	17.0
64	17.5
65	18.0
66	16.0
67	14.0
68	11.0
69	8.0
70	6.0
71	4.0
72	3.5
73	3.0
74	2.5
75	2.5
76	3.0
77	1.5
78	0.0
79	1.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.314439599897412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.32163742690058	25.08335470633496
2	2.631578947368421	1.3849705052577583
3	0.4873294346978557	0.3847140292382662
4	0.29239766081871343	0.30777122339061297
5	0.3898635477582846	0.5129520389843549
6	0.09746588693957114	0.15388561169530648
7	0.0	0.0
8	0.0	0.0
9	0.09746588693957114	0.23082841754295974
>10	0.4873294346978557	1.923570146191331
>50	0.0	0.0
>100	0.09746588693957114	4.462682739163888
>500	0.0	0.0
>1k	0.09746588693957114	65.55527058220056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC	2556	65.55527058220056	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT	174	4.462682739163888	RNA PCR Primer, Index 44 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT	20	0.5129520389843549	RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG	19	0.4873044370351372	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	14	0.3590664272890485	No Hit
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT	12	0.30777122339061297	RNA PCR Primer, Index 37 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC	10	0.25647601949217746	RNA PCR Primer, Index 37 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATATCGTATGCCGTC	9	0.23082841754295974	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	6	0.15388561169530648	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTAGAATATCTCGTATGCCGTC	5	0.12823800974608873	RNA PCR Primer, Index 44 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	5	0.12823800974608873	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTAGTATGCCGTC	5	0.12823800974608873	No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA	5	0.12823800974608873	No Hit
CGGAGATGTGTATAAGAGACAGTTATACACATCTCCGAGCCCACGAGACCC	4	0.10259040779687098	No Hit
CTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT	4	0.10259040779687098	RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAAGATCTCGTATGCCGTC	4	0.10259040779687098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0512952038984355
18	0.0	0.0	0.102590407796871
19	0.0	0.0	0.15388561169530648
20	0.0	0.0	0.15388561169530648
21	0.0	0.0	0.15388561169530648
22	0.0	0.0	0.15388561169530648
23	0.0	0.0	0.17953321364452424
24	0.0	0.0	0.17953321364452424
25	0.0	0.0	0.205180815593742
26	0.0	0.0	0.205180815593742
27	0.0	0.0	0.25647601949217746
28	0.0	0.0	0.30777122339061297
29	0.0	0.0	0.3847140292382662
30	0.0	0.0	0.4360092331367017
31	0.0	0.0	0.6155424467812259
32	0.0	0.0	0.820723262374968
33	0.0	0.0	1.000256476019492
34	0.0	0.0	1.3593229033085406
35	0.0	0.0	1.718389330597589
36	0.0	0.0	2.1800461656835086
37	0.0	0.0	2.539112592972557
38	0.0	0.0	3.1033598358553474
39	0.0	0.0	3.565016670941267
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	275	0.0	42.545452	45
ATGCCGT	280	0.0	41.785713	44
CTTATAC	285	0.0	41.05263	1
AGCCCAC	295	0.0	39.66102	17
GTATGCC	295	0.0	39.66102	42
ATCTCCG	295	0.0	39.66102	10
CACATCT	295	0.0	39.66102	7
GCCCACG	295	0.0	39.66102	18
CCACGAG	295	0.0	39.66102	20
TATGCCG	295	0.0	39.66102	43
CATCTCC	295	0.0	39.66102	9
GAGCCCA	295	0.0	39.66102	16
CACGAGA	295	0.0	39.66102	21
CCCACGA	295	0.0	39.66102	19
ACATCTC	295	0.0	39.66102	8
ACGAGAC	295	0.0	39.66102	22
CGTATGC	295	0.0	39.66102	41
CGAGCCC	295	0.0	39.66102	15
CCGAGCC	295	0.0	39.66102	14
TCGTATG	295	0.0	39.66102	40
>>END_MODULE