FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_3.341000000074dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_3.341000000074dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC255665.55527058220056No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1744.462682739163888RNA PCR Primer, Index 44 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT200.5129520389843549RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG190.4873044370351372No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC140.3590664272890485No Hit
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT120.30777122339061297RNA PCR Primer, Index 37 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC100.25647601949217746RNA PCR Primer, Index 37 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATATCGTATGCCGTC90.23082841754295974No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC60.15388561169530648No Hit
CTTATACACATCTCCGAGCCCACGAGACACTAGAATATCTCGTATGCCGTC50.12823800974608873RNA PCR Primer, Index 44 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.12823800974608873RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTAGTATGCCGTC50.12823800974608873No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA50.12823800974608873No Hit
CGGAGATGTGTATAAGAGACAGTTATACACATCTCCGAGCCCACGAGACCC40.10259040779687098No Hit
CTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT40.10259040779687098RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAAGATCTCGTATGCCGTC40.10259040779687098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2750.042.54545245
ATGCCGT2800.041.78571344
CTTATAC2850.041.052631
AGCCCAC2950.039.6610217
GTATGCC2950.039.6610242
ATCTCCG2950.039.6610210
CACATCT2950.039.661027
GCCCACG2950.039.6610218
CCACGAG2950.039.6610220
TATGCCG2950.039.6610243
CATCTCC2950.039.661029
GAGCCCA2950.039.6610216
CACGAGA2950.039.6610221
CCCACGA2950.039.6610219
ACATCTC2950.039.661028
ACGAGAC2950.039.6610222
CGTATGC2950.039.6610241
CGAGCCC2950.039.6610215
CCGAGCC2950.039.6610214
TCGTATG2950.039.6610240
Produced by FastQC (version 0.11.2)