##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 0h_29.341000000076a5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5317
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.42542787286064	38.0	32.0	38.0	32.0	38.0
2	35.598833928907275	38.0	32.0	38.0	32.0	38.0
3	35.52886966334399	38.0	32.0	38.0	32.0	38.0
4	35.68722964077487	38.0	32.0	38.0	32.0	38.0
5	35.9185630994922	38.0	32.0	38.0	32.0	38.0
6	37.495204062441225	38.0	38.0	40.0	32.0	40.0
7	37.33233026142562	38.0	38.0	40.0	32.0	40.0
8	37.226067331201804	38.0	38.0	40.0	32.0	40.0
9	37.288132405491815	38.0	38.0	40.0	32.0	40.0
10	37.14500658265939	38.0	38.0	40.0	32.0	40.0
11	37.26669174346436	38.0	38.0	40.0	32.0	40.0
12	37.209328568741775	38.0	38.0	40.0	32.0	40.0
13	37.292834305059245	38.0	38.0	40.0	32.0	40.0
14	36.996050404363366	38.0	38.0	40.0	32.0	40.0
15	37.05303742712056	38.0	38.0	40.0	32.0	40.0
16	36.80778634568366	38.0	38.0	40.0	32.0	40.0
17	36.94921948467181	38.0	38.0	40.0	32.0	40.0
18	36.894677449689674	38.0	38.0	40.0	32.0	40.0
19	37.02200488997555	38.0	38.0	40.0	32.0	40.0
20	36.87586985141997	38.0	38.0	40.0	32.0	40.0
21	37.06676697385744	38.0	38.0	40.0	32.0	40.0
22	36.93586608990032	38.0	38.0	40.0	32.0	40.0
23	37.0692119616325	38.0	38.0	40.0	32.0	40.0
24	36.991724656761335	38.0	38.0	40.0	32.0	40.0
25	36.93191649426368	38.0	38.0	40.0	32.0	40.0
26	36.754372766597704	38.0	38.0	40.0	32.0	40.0
27	36.739326687981944	38.0	38.0	40.0	32.0	40.0
28	36.83975926274215	38.0	38.0	40.0	32.0	40.0
29	36.82508933609178	38.0	38.0	40.0	32.0	40.0
30	36.81982320857627	38.0	38.0	40.0	32.0	40.0
31	36.817190144818504	38.0	38.0	40.0	32.0	40.0
32	36.697197667857814	38.0	38.0	40.0	32.0	40.0
33	36.81568553695693	38.0	38.0	40.0	32.0	40.0
34	36.78822644348317	38.0	38.0	40.0	32.0	40.0
35	36.84991536580779	38.0	38.0	40.0	32.0	40.0
36	36.748542411134096	38.0	38.0	40.0	32.0	40.0
37	36.939439533571566	38.0	38.0	40.0	32.0	40.0
38	36.86514952040624	38.0	38.0	40.0	32.0	40.0
39	36.81342862516457	38.0	38.0	40.0	32.0	40.0
40	36.80252021816814	38.0	38.0	40.0	32.0	40.0
41	36.8049652059432	38.0	38.0	40.0	32.0	40.0
42	36.752680082753436	38.0	38.0	40.0	32.0	40.0
43	36.8557457212714	38.0	38.0	40.0	32.0	40.0
44	36.74045514387813	38.0	38.0	40.0	32.0	40.0
45	36.85969531690803	38.0	38.0	40.0	32.0	40.0
46	36.573067519277785	38.0	38.0	40.0	32.0	40.0
47	36.68045890539778	38.0	38.0	40.0	32.0	40.0
48	36.63212337784465	38.0	38.0	40.0	32.0	40.0
49	36.6101184878691	38.0	38.0	40.0	32.0	40.0
50	36.50385555764529	38.0	38.0	40.0	32.0	40.0
51	35.91066390821892	38.0	38.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	-3.606153612012985
2201	2	-2.199635303932162
2201	3	-0.20645461309523228
2201	4	2.2559140512265614
2201	5	2.0907202606421293
2201	6	1.3526368596681166
2201	7	1.7957065070346445
2201	8	0.26567009379509443
2201	9	0.23999312319624266
2201	10	1.8820667613636246
2201	11	-1.5414840367965326
2201	12	1.2804129464285765
2201	13	0.8729228670634868
2201	14	-1.6100142045454575
2201	15	0.5151746257215066
2201	16	-0.4569906655844136
2201	17	2.1606601731601813
2201	18	0.9254323367604727
2201	19	1.007955109126982
2201	20	1.2853693181818144
2201	21	1.3456760687229519
2201	22	1.0893674467893177
2201	23	-5.194760101010104
2201	24	0.7240981240981128
2201	25	0.42745592081529793
2201	26	-1.239741725288603
2201	27	-2.1357740349927994
2201	28	-2.964694376803756
2201	29	-0.993620355339111
2201	30	-2.3026503878066293
2201	31	0.3620174963924967
2201	32	-1.184798318001441
2201	33	0.40508037968974975
2201	34	0.5329832927489306
2201	35	-3.523453846500729
2201	36	1.3225271690115363
2201	37	1.7206631042568574
2201	38	2.35590841450216
2201	39	-1.9430200441919254
2201	40	-1.8873354076479245
2201	41	-2.3350587346681024
2201	42	0.5695509785353394
2201	43	0.5667151424963848
2201	44	0.24936417748917705
2201	45	0.6467087166305916
2201	46	-1.8240152642496241
2201	47	-0.08945707070706987
2201	48	-1.2607159767316034
2201	49	-2.8829246708152994
2201	50	-1.9345767947330472
2201	51	1.0110305059523697
1101	1	0.6478146419552715
1101	2	1.990840886544028
1101	3	-0.3334387400793588
1101	4	-1.1885303932178815
1101	5	0.7573869273087936
1101	6	2.0510495580808197
1101	7	0.4306271419552772
1101	8	-1.1946473665223607
1101	9	-1.6647687815656624
1101	10	-1.8163459370490784
1101	11	0.8235953282828348
1101	12	2.105809771825406
1101	13	1.6983196924603163
1101	14	-0.40366499819624835
1101	15	-0.05625394570706277
1101	16	0.923961715367966
1101	17	-2.5377525252525217
1101	18	-3.296789885461749
1101	19	-0.7222036210317455
1101	20	0.7774328102453083
1101	21	1.8853586084054967
1101	22	2.35920871663059
1101	23	0.4242875180375165
1101	24	1.8193362193362077
1101	25	0.9988844922438673
1101	26	1.6332741477272705
1101	27	-0.8976787968975657
1101	28	-1.869456281565661
1101	29	1.2286018668831105
1101	30	1.9354448502886044
1101	31	1.5207476551226549
1101	32	2.1961540629509386
1101	33	1.7860327606421293
1101	34	1.6282213879870255
1101	35	1.6511493281024414
1101	36	-0.05842521194084327
1101	37	1.0857424693362248
1101	38	-0.7393296807359349
1101	39	0.5014244002525174
1101	40	-1.7286052489177663
1101	41	0.6173222177128466
1101	42	-3.160607751623388
1101	43	-1.8936023178210704
1101	44	-0.36968344155844335
1101	45	-1.2580531881313135
1101	46	-2.9827454229797823
1101	47	-1.4069173881673862
1101	48	0.1202364042207762
1101	49	-0.7083214962121289
1101	50	-0.1409260010822564
1101	51	1.63007812499999
2202	1	2.5367035308441572
2202	2	-0.4424924467893092
2202	3	-0.07788318452379883
2202	4	0.45591405122656425
2202	5	0.3907202606421265
2202	6	0.7954940025252597
2202	7	2.052849364177497
2202	8	2.0942415223665236
2202	9	1.868564551767676
2202	10	2.1392096185064773
2202	11	1.8013731060606162
2202	12	2.3946986607142904
2202	13	1.787208581349205
2202	14	2.018557224025976
2202	15	2.14374605429294
2202	16	1.6572950487013003
2202	17	1.8178030303030326
2202	18	2.1254323367604755
2202	19	2.122240823412696
2202	20	1.5996550324675312
2202	21	1.7742474972943825
2202	22	2.003653161075036
2202	23	-0.20904581529581634
2202	24	1.4526695526695406
2202	25	1.1988844922438702
2202	26	1.788829703282829
2202	27	2.635654536435773
2202	28	2.8638770517676733
2202	29	-0.8936203553391096
2202	30	1.468778183621943
2202	31	2.076303210678212
2202	32	1.2294873962842772
2202	33	1.7193660939754665
2202	34	1.3615547213203598
2202	35	0.9622604392135585
2202	36	2.0082414547258196
2202	37	1.6635202471140005
2202	38	2.1273369859307323
2202	39	1.656979955808076
2202	40	2.2269503066377894
2202	41	1.5506555510461837
2202	42	1.4838366928210576
2202	43	0.0952865710678168
2202	44	1.9636498917748924
2202	45	-0.6247198547979806
2202	46	3.061699021464662
2202	47	3.081971500721501
2202	48	2.8535697375541105
2202	49	-0.09721038510101465
2202	50	2.0368517766955208
2202	51	0.19674479166665293
1102	1	-2.7132964691558428
1102	2	0.5325075532106922
1102	3	-1.6528831845238017
1102	4	-4.494085948773439
1102	5	-0.7842797393578707
1102	6	-0.004505997474737455
1102	7	-2.9721506358225014
1102	8	-2.180758477633475
1102	9	-0.9564354482323267
1102	10	-0.8857903814935213
1102	11	1.3513731060606133
1102	12	-2.380301339285708
1102	13	1.087208581349202
1102	14	0.5685572240259731
1102	15	1.9437460542929372
1102	16	2.2572950487013017
1102	17	2.5178030303030354
1102	18	2.1754323367604727
1102	19	1.4722408234126974
1102	20	1.2496550324675297
1102	21	0.7742474972943825
1102	22	1.553653161075033
1102	23	-1.2840458152958192
1102	24	1.9026695526695434
1102	25	-0.6261155077561327
1102	26	0.06382970328282767
1102	27	0.68565453643577
1102	28	1.2138770517676747
1102	29	2.006379644660889
1102	30	-0.35622181637805994
1102	31	1.326303210678212
1102	32	0.7794873962842743
1102	33	1.619366093975465
1102	34	1.9615547213203612
1102	35	1.5122604392135557
1102	36	1.108241454725821
1102	37	0.8635202471140033
1102	38	-3.8226630140692706
1102	39	1.8069799558080746
1102	40	1.5769503066377908
1102	41	-0.29934444895381773
1102	42	-3.2161633071789453
1102	43	-3.2547134289321846
1102	44	0.21364989177489235
1102	45	1.5752801452020222
1102	46	1.7116990214646606
1102	47	1.7319715007214995
1102	48	-1.671430262445888
1102	49	-0.34721038510101465
1102	50	-1.2381482233044778
1102	51	-0.8282552083333456
2203	1	0.13670353084415865
2203	2	-1.3424924467893078
2203	3	0.42211681547620117
2203	4	1.65591405122656
2203	5	0.2907202606421322
2203	6	-0.004505997474737455
2203	7	1.8528493641775015
2203	8	1.694241522366525
2203	9	-0.33143544823232673
2203	10	-2.7607903814935213
2203	11	1.2013731060606148
2203	12	0.9946986607142918
2203	13	-4.012791418650799
2203	14	-1.0814427759740255
2203	15	-0.7562539457070656
2203	16	-1.4427049512987011
2203	17	-1.2821969696969617
2203	18	0.9254323367604727
2203	19	0.5222408234126945
2203	20	0.9996550324675297
2203	21	-1.7257525027056175
2203	22	-0.2963468389249684
2203	23	1.890954184704178
2203	24	1.1526695526695434
2203	25	0.7988844922438645
2203	26	0.08882970328282624
2203	27	-0.6643454635642314
2203	28	1.3638770517676733
2203	29	0.3063796446608862
2203	30	0.9687781836219429
2203	31	1.2763032106782148
2203	32	1.5294873962842743
2203	33	0.7193660939754665
2203	34	1.7615547213203584
2203	35	-1.9377395607864472
2203	36	1.5082414547258196
2203	37	1.4635202471140047
2203	38	0.827336985930728
2203	39	1.656979955808076
2203	40	1.2269503066377894
2203	41	1.5506555510461837
2203	42	-0.6161633071789439
2203	43	0.5952865710678168
2203	44	0.1636498917748952
2203	45	-0.3247198547979764
2203	46	1.8616990214646592
2203	47	-0.8180284992784976
2203	48	1.8535697375541105
2203	49	2.0027896148989868
2203	50	0.43685177669551933
2203	51	0.29674479166665435
1103	1	0.6478146419552715
1103	2	1.3241742198773565
1103	3	0.3332279265873126
1103	4	-0.4107526154401029
1103	5	-0.4648352949134278
1103	6	-1.3933948863636232
1103	7	-0.013817302489165684
1103	8	-3.750202922077918
1103	9	-0.33143544823232673
1103	10	1.405876285173143
1103	11	0.8235953282828348
1103	12	1.105809771825406
1103	13	1.031653025793645
1103	14	1.0407794462481945
1103	15	1.832634943181823
1103	16	1.923961715367966
1103	17	2.1289141414141497
1103	18	-0.18567877435064162
1103	19	0.7222408234126974
1103	20	1.4440994769119726
1103	21	0.3298030528499396
1103	22	1.0258753832972545
1103	23	0.7576208513708451
1103	24	-2.2917748917748995
1103	25	-2.4455599522005755
1103	26	0.5221630366161634
1103	27	2.1023212031024343
1103	28	2.3527659406565604
1103	29	1.006379644660889
1103	30	1.713222628066383
1103	31	-0.8125856782106737
1103	32	0.5294873962842743
1103	33	-0.5473005726911992
1103	34	-2.4828897231240816
1103	35	-2.682184005230887
1103	36	-1.391758545274179
1103	37	0.8635202471140033
1103	38	-1.8504407918470491
1103	39	-1.720797821969704
1103	40	-1.061938582251095
1103	41	-1.8271222267315963
1103	42	-0.49394108495672384
1103	43	-0.4491578733766275
1103	44	-0.703016774891772
1103	45	-1.035830965909092
1103	46	0.9061434659091034
1103	47	-1.0735840548340576
1103	48	-1.4353191513347738
1103	49	-0.9305437184343504
1103	50	-0.918703778860035
1103	51	-0.8143663194444599
2204	1	0.28670353084415723
2204	2	1.9075075532106922
2204	3	0.7221168154761983
2204	4	0.005914051226561412
2204	5	1.3407202606421293
2204	6	0.7454940025252625
2204	7	2.1528493641774986
2204	8	1.944241522366525
2204	9	1.9185645517676733
2204	10	1.4892096185064787
2204	11	1.8513731060606133
2204	12	0.4946986607142918
2204	13	1.587208581349202
2204	14	0.4435572240259731
2204	15	1.1937460542929372
2204	16	1.5072950487013017
2204	17	1.5178030303030354
2204	18	2.1754323367604727
2204	19	1.2222408234126974
2204	20	1.7496550324675297
2204	21	0.7742474972943825
2204	22	1.053653161075033
2204	23	-1.7840458152958192
2204	24	1.0276695526695434
2204	25	2.2488844922438673
2204	26	2.4388297032828277
2204	27	2.43565453643577
2204	28	1.7138770517676747
2204	29	0.631379644660889
2204	30	2.01877818362194
2204	31	0.076303210678212
2204	32	-0.7205126037157257
2204	33	0.869366093975465
2204	34	0.5865547213203612
2204	35	1.7622604392135557
2204	36	2.608241454725821
2204	37	1.6135202471140033
2204	38	0.6773369859307294
2204	39	0.8069799558080746
2204	40	1.0769503066377908
2204	41	1.7006555510461823
2204	42	1.7838366928210547
2204	43	1.7452865710678154
2204	44	1.4636498917748924
2204	45	1.8252801452020222
2204	46	1.4616990214646606
2204	47	1.7319715007214995
2204	48	0.7035697375541119
2204	49	2.6527896148989853
2204	50	-0.9881482233044778
2204	51	1.5467447916666544
2205	1	-0.33829646915584277
2205	2	1.1575075532106922
2205	3	1.4721168154761983
2205	4	-1.4940859487734386
2205	5	0.5907202606421293
2205	6	1.2454940025252625
2205	7	1.6528493641774986
2205	8	1.444241522366525
2205	9	-1.4564354482323267
2205	10	0.9892096185064787
2205	11	1.1013731060606133
2205	12	0.2446986607142918
2205	13	1.587208581349202
2205	14	-1.806442775974027
2205	15	0.9437460542929372
2205	16	1.5072950487013017
2205	17	1.2678030303030354
2205	18	0.42543233676047265
2205	19	0.7222408234126974
2205	20	-5.62534496753247
2205	21	-1.4757525027056175
2205	22	0.17865316107503304
2205	23	2.090954184704181
2205	24	1.9026695526695434
2205	25	0.9988844922438673
2205	26	2.1888297032828277
2205	27	2.68565453643577
2205	28	1.9638770517676747
2205	29	2.006379644660889
2205	30	1.76877818362194
2205	31	2.076303210678212
2205	32	2.7794873962842743
2205	33	2.119366093975465
2205	34	1.9615547213203612
2205	35	0.2622604392135557
2205	36	-1.266758545274179
2205	37	-0.3864797528859967
2205	38	0.17733698593072944
2205	39	2.0569799558080746
2205	40	0.07695030663779079
2205	41	1.2006555510461823
2205	42	2.0338366928210547
2205	43	1.2452865710678154
2205	44	1.2136498917748924
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1211	8	0.5831304112554108
1211	9	0.7796756628787875
1211	10	1.850320729617593
1211	11	1.2680397727272776
1211	12	1.8835875496031775
1211	13	0.5872085813492021
1211	14	-0.8481094426406912
1211	15	0.832634943181823
1211	16	3.0350728264790803
1211	17	2.351136363636371
1211	18	-0.2967898854617488
1211	19	1.3889074900793617
1211	20	2.110766143578644
1211	21	1.6631363861832682
1211	22	-0.19634683892496696
1211	23	2.4242875180375165
1211	24	1.1526695526695434
1211	25	-0.44555995220057554
1211	26	1.411051925505049
1211	27	0.8800989808802129
1211	28	2.574988162878789
1211	29	2.1174907557720033
1211	30	1.713222628066383
1211	31	-0.36814123376623087
1211	32	2.86282072961761
1211	33	1.563810538419908
1211	34	2.294888054653697
1211	35	-0.6821840052308872
1211	36	0.3860192325035996
1211	37	1.196853580447339
1211	38	2.260670319264065
1211	39	2.3903132891414103
1211	40	1.938061417748905
1211	41	2.283988884379518
1211	42	2.6171700261543904
1211	43	2.5508421266233725
1211	44	1.6303165584415567
1211	45	2.408613478535358
1211	46	2.7950323547979963
1211	47	2.593082611832614
1211	48	2.7869030708874476
1211	49	1.6250118371212068
1211	50	2.525740665584408
1211	51	2.9634114583333186
1208	1	-0.46329646915584277
1208	2	-0.3424924467893078
1208	3	2.2221168154761983
1208	4	0.005914051226561412
1208	5	0.5907202606421293
1208	6	0.7454940025252625
1208	7	-1.4721506358225014
1208	8	0.944241522366525
1208	9	1.6685645517676733
1208	10	1.7392096185064787
1208	11	-0.14862689393938666
1208	12	1.9946986607142918
1208	13	0.46220858134920206
1208	14	-0.6814427759740269
1208	15	-4.806253945707063
1208	16	-4.242704951298698
1208	17	-2.1071969696969646
1208	18	-1.6995676632395273
1208	19	0.09724082341269735
1208	20	-2.3753449675324703
1208	21	-0.10075250270561753
1208	22	-0.07134683892496696
1208	23	1.0909541847041808
1208	24	-1.2223304473304566
1208	25	-2.5011155077561327
1208	26	-1.6861702967171723
1208	27	0.81065453643577
1208	28	-4.536122948232325
1208	29	-2.743620355339111
1208	30	2.51877818362194
1208	31	1.576303210678212
1208	32	-1.9705126037157257
1208	33	-1.505633906024535
1208	34	-1.9134452786796388
1208	35	0.7622604392135557
1208	36	-0.14175854527417897
1208	37	-2.7614797528859967
1208	38	1.6773369859307294
1208	39	0.3069799558080746
1208	40	0.8269503066377908
1208	41	1.7006555510461823
1208	42	1.5338366928210547
1208	43	2.2452865710678154
1208	44	0.46364989177489235
1208	45	-1.2997198547979778
1208	46	-1.4133009785353394
1208	47	0.8569715007214995
1208	48	0.2035697375541119
1208	49	1.1527896148989853
1208	50	2.136851776695522
1208	51	-0.32825520833334565
1209	1	1.7867035308441572
1209	2	0.5325075532106922
1209	3	1.4721168154761983
1209	4	2.2559140512265614
1209	5	-1.0342797393578707
1209	6	-2.3795059974747375
1209	7	-0.7221506358225014
1209	8	-0.18075847763347497
1209	9	1.1685645517676733
1209	10	0.4892096185064787
1209	11	0.7263731060606133
1209	12	-0.8803013392857082
1209	13	0.08720858134920206
1209	14	-0.4314427759740269
1209	15	-2.3062539457070628
1209	16	-0.6177049512986983
1209	17	0.7678030303030354
1209	18	0.6754323367604727
1209	19	-0.15275917658730265
1209	20	-0.12534496753247026
1209	21	-1.1007525027056175
1209	22	0.42865316107503304
1209	23	2.340954184704181
1209	24	1.9026695526695434
1209	25	2.2488844922438673
1209	26	-0.9361702967171723
1209	27	-0.43934546356423
1209	28	1.5888770517676747
1209	29	0.756379644660889
1209	30	2.76877818362194
1209	31	1.576303210678212
1209	32	2.0294873962842743
1209	33	1.119366093975465
1209	34	1.7115547213203612
1209	35	-0.8627395607864443
1209	36	-4.641758545274179
1209	37	-4.386479752885997
1209	38	-1.1976630140692706
1209	39	0.18197995580807458
1209	40	0.9519503066377908
1209	41	2.4506555510461823
1209	42	2.5338366928210547
1209	43	1.2452865710678154
1209	44	2.2136498917748924
1209	45	2.575280145202022
1209	46	1.7116990214646606
1209	47	2.9819715007214995
1209	48	2.203569737554112
1209	49	1.2777896148989853
1209	50	1.6368517766955222
1209	51	1.9217447916666544
1214	1	-3.606153612012985
1214	2	-3.342492446789308
1214	3	-5.492168898809517
1214	4	-0.315514520202008
1214	5	-0.48070831078644005
1214	6	1.066922573953832
1214	7	0.9385636498917833
1214	8	0.9799558080808097
1214	9	-3.188578305375181
1214	10	-0.6893618100649519
1214	11	1.1728016774891827
1214	12	-3.7195870535714235
1214	13	-2.412791418650798
1214	14	-2.4671570616883116
1214	15	-3.1991110885642087
1214	16	-2.885562094155844
1214	17	-0.4107683982683952
1214	18	-2.2174248060966733
1214	19	0.15081225198412795
1214	20	-0.42891639610390087
1214	21	-3.3686096455627634
1214	22	-2.196346838924967
1214	23	0.5195256132756114
1214	24	-7.4187590187590295
1214	25	-2.8582583648989868
1214	26	-1.6683131538600264
1214	27	-2.421488320707084
1214	28	0.17816276605339
1214	29	-3.136477498196257
1214	30	-0.01693610209234464
1214	31	-1.066553932178934
1214	32	0.10091596771284372
1214	33	-0.737776763167389
1214	34	-3.752730992965354
1214	35	0.1908318677849863
1214	36	-1.6774728309884637
1214	37	-0.5650511814574273
1214	38	-2.9298058712121247
1214	39	0.4855513843795052
1214	40	0.2555217352092214
1214	41	-2.906487306096672
1214	42	-2.00187759289323
1214	43	-0.2904277146464693
1214	44	-3.1792072510822535
1214	45	-3.0675769976551237
1214	46	0.3188418786075147
1214	47	-2.6608856421356464
1214	48	-2.689287405303034
1214	49	0.831361043470416
1214	50	-4.6488625090187625
1214	51	-3.5603980654761997
1215	1	-1.3521853580447285
1215	2	-0.00915911345597209
1215	3	-0.44454985119046597
1215	4	-1.1885303932178815
1215	5	0.20183137175324362
1215	6	-2.0600615530302946
1215	7	0.87507158639972
1215	8	-1.1946473665223607
1215	9	1.3352312184343376
1215	10	2.0725429518398144
1215	11	0.8235953282828348
1215	12	0.43914310515873467
1215	13	-1.5239025297619122
1215	14	-0.18144277597402692
1215	15	0.16596827651515866
1215	16	-1.2982605068542554
1215	17	-0.204419191919186
1215	18	0.592099003427137
1215	19	0.2777963789682545
1215	20	-3.444789411976913
1215	21	-0.8924191693722818
1215	22	0.6925420499639188
1215	23	0.09095418470418082
1215	24	-0.29177489177489946
1215	25	-2.3344488410894684
1215	26	0.7443852588383848
1215	27	-3.4532343524531157
1215	28	-2.0916785037878824
1215	29	0.22860186688311046
1215	30	-4.064555149711396
1215	31	-3.1459190115440094
1215	32	-4.13717927038239
1215	33	-6.547300572691203
1215	34	-5.371778612012971
1215	35	-3.3488506718975586
1215	36	-5.280647434163068
1215	37	1.3079646915584462
1215	38	1.5940036525973937
1215	39	-3.831908933080811
1215	40	1.938061417748905
1215	41	-1.3826777822871534
1215	42	2.394947803932169
1215	43	-3.4491578733766275
1215	44	-3.703016774891772
1215	45	-4.591386521464642
1215	46	-0.09385653409089656
1215	47	1.3708603896103853
1215	48	-0.6575413735570024
1215	49	-0.9305437184343504
1215	50	-0.1409260010822564
1215	51	-3.0365885416666814
1212	1	0.5367035308441572
1212	2	0.6575075532106922
1212	3	1.2221168154761983
1212	4	0.2559140512265614
1212	5	0.09072026064212935
1212	6	-3.0045059974747375
1212	7	1.319516030844163
1212	8	2.0275748556998607
1212	9	-0.6647687815656624
1212	10	0.0725429518398144
1212	11	0.6013731060606133
1212	12	1.3280319940476275
1212	13	0.5872085813492021
1212	14	1.818557224025973
1212	15	1.9437460542929372
1212	16	1.2572950487013017
1212	17	-2.4821969696969646
1212	18	0.9254323367604727
1212	19	-2.1110925099206383
1212	20	0.49965503246752974
1212	21	-0.39241916937228183
1212	22	-2.3630135055916313
1212	23	2.090954184704181
1212	24	2.486002886002879
1212	25	1.9988844922438673
1212	26	1.855496369949492
1212	27	2.43565453643577
1212	28	1.4638770517676747
1212	29	1.006379644660889
1212	30	2.26877818362194
1212	31	2.076303210678212
1212	32	1.5294873962842743
1212	33	2.119366093975465
1212	34	2.6282213879870255
1212	35	2.0955937725468914
1212	36	2.9415747880591567
1212	37	1.8635202471140033
1212	38	2.5940036525973937
1212	39	2.0569799558080746
1212	40	0.1602836399711265
1212	41	1.9506555510461823
1212	42	2.6171700261543904
1212	43	1.9952865710678154
1212	44	0.6303165584415567
1212	45	2.075280145202022
1212	46	2.7950323547979963
1212	47	2.4819715007214995
1212	48	2.120236404220776
1212	49	2.4027896148989853
1212	50	2.970185110028858
1212	51	3.63007812499999
1213	1	0.5367035308441572
1213	2	-0.1758257801226435
1213	3	1.2221168154761983
1213	4	0.2559140512265614
1213	5	0.09072026064212935
1213	6	1.4954940025252625
1213	7	1.6528493641774986
1213	8	2.3609081890331893
1213	9	1.001897885101009
1213	10	0.7392096185064787
1213	11	1.934706439393949
1213	12	1.3280319940476275
1213	13	1.9205419146825378
1213	14	0.1518905573593088
1213	15	0.9437460542929372
1213	16	-1.9093716179653626
1213	17	-0.8155303030303003
1213	18	-2.0745676632395273
1213	19	2.055574156746033
1213	20	1.9996550324675297
1213	21	-0.5590858360389532
1213	22	0.803653161075033
1213	23	1.0909541847041808
1213	24	-1.6806637806637923
1213	25	0.49888449224386733
1213	26	-1.9778369633838366
1213	27	-1.3976787968975657
1213	28	-1.7027896148989896
1213	29	-2.1602870220057753
1213	30	-1.23122181637806
1213	31	0.2429698773448763
1213	32	1.0294873962842743
1213	33	-1.7139672393578707
1213	34	-1.8717786120129745
1213	35	-1.4044062274531086
1213	36	-2.891758545274179
1213	37	-2.303146419552661
1213	38	-3.239329680735935
1213	39	-0.7763533775252611
1213	40	1.8269503066377908
1213	41	1.283988884379518
1213	42	-1.549496640512281
1213	43	-2.171380095598849
1213	44	-2.5363501082251076
1213	45	1.7419468118686865
1213	46	0.4616990214646606
1213	47	-3.851361832611836
1213	48	-2.7130969291125524
1213	49	-1.4305437184343504
1213	50	-6.029814889971146
1213	51	3.2967447916666544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	0.0
22	5.0
23	6.0
24	19.0
25	32.0
26	35.0
27	58.0
28	99.0
29	113.0
30	125.0
31	161.0
32	176.0
33	192.0
34	259.0
35	270.0
36	400.0
37	543.0
38	901.0
39	1921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.391795257809555	17.689123071132855	13.116296575084682	31.8027850959729
2	14.651119052097048	20.18055294338913	42.053789731051346	23.11453827346248
3	16.531878879067143	24.11134098175663	35.67801391762272	23.678766221553506
4	12.130900883957118	22.625540718450253	35.433515140116604	29.81004325747602
5	12.920820011284558	35.01974797818318	38.42392326499906	13.635508745533196
6	26.123753996614635	42.22305811547865	18.63832988527365	13.014858002633064
7	24.600338536768852	31.93530186195223	20.70716569494076	22.75719390633816
8	22.73838630806846	39.92853112657514	19.108519842016175	18.224562723340227
9	24.224186571374837	16.174534511942827	19.559902200489	40.04137671619334
10	15.384615384615385	31.709610682715816	30.03573443671243	22.870039495956366
11	30.76923076923077	23.772804212902013	27.985706225315027	17.472258792552193
12	20.78239608801956	24.186571374835435	29.302238104194096	25.728794432950913
13	26.70678954297536	20.95166447244687	31.389881512130902	20.95166447244687
14	21.290201241301485	20.744780891480158	26.65036674816626	31.314651119052094
15	21.703968403234907	27.910475832236227	23.772804212902013	26.612751551626857
16	26.744404739514767	26.970095918751174	23.923264999059622	22.36223434267444
17	21.13974045514388	31.803648674064323	24.90126010908407	22.15535076170773
18	26.617757712565837	26.993980436418358	25.99699021820918	20.39127163280662
19	25.12695128832048	24.036110588677825	25.05172089524168	25.785217227760015
20	23.133345871732182	24.374647357532442	24.017302990408123	28.474703780327253
21	22.36223434267444	25.747602031220612	25.67237163814181	26.217791987963135
22	22.080120368628926	30.110964829791236	24.90126010908407	22.90765469249577
23	21.327816437840887	25.371450065826593	26.537521158548056	26.763212337784463
24	27.628361858190708	25.33383486928719	25.785217227760015	21.252586044762083
25	22.475079932292648	30.317848410757946	26.067331201805526	21.13974045514388
26	26.85725032913297	26.161369193154034	25.67237163814181	21.309008839571185
27	22.098927966898625	32.06695504984013	25.07052849351138	20.76358848974986
28	21.685160804965207	26.387060372390447	24.75079932292646	27.176979499717884
29	24.84483731427497	25.70998683468121	27.590746661651306	21.854429189392516
30	24.604966139954854	26.993980436418358	26.768246802106848	21.63280662151994
31	21.609930411886403	25.653564039872105	28.343050592439344	24.393454955802145
32	21.892044385931918	24.242994169644536	30.110964829791236	23.75399661463231
33	22.625540718450253	25.70998683468121	29.132969719766788	22.53150272710175
34	21.6475456084258	25.29621967274779	31.4086891104006	21.6475456084258
35	22.71957870979876	25.07052849351138	28.41828098551815	23.791611811171716
36	21.19616324995298	24.84483731427497	30.261425615948845	23.69757381982321
37	19.559902200489	28.02332142185443	27.81643784088772	24.600338536768852
38	18.958059055858566	21.534700018807598	34.66240361105887	24.84483731427497
39	20.236975738198232	21.083317660334775	29.3398533007335	29.3398533007335
40	18.581907090464547	21.77919879631371	37.1450065826594	22.493887530562347
41	19.99247696069212	20.895241677637767	30.336656009027646	28.77562535264247
42	22.870039495956366	20.650742900131654	32.51833740831296	23.96088019559902
43	17.190144818506678	21.421854429189395	35.82847470378033	25.5595260485236
44	16.268572503291328	27.647169456460407	32.25503103253715	23.829227007711115
45	15.6667293586609	22.681963513259358	36.97573819823209	24.675568929847657
46	21.271393643031786	23.246191461350385	31.991724656761335	23.490690238856498
47	14.763964641715251	22.230581154786535	32.78164378408877	30.223810419409443
48	14.594696257287945	23.415459845777693	31.01372954673688	30.97611435019748
49	19.146135038555574	22.249388753056234	32.744028587549366	25.860447620838816
50	13.93643031784841	24.036110588677825	35.2642467556893	26.763212337784463
51	13.54147075418469	23.189768666541283	29.490314086891107	33.778446492382926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.5
22	5.0
23	5.0
24	5.0
25	4.0
26	7.0
27	11.0
28	22.5
29	34.0
30	59.0
31	84.0
32	101.0
33	118.0
34	141.5
35	165.0
36	204.0
37	243.0
38	278.5
39	314.0
40	381.5
41	449.0
42	510.0
43	571.0
44	582.5
45	594.0
46	638.5
47	683.0
48	717.5
49	752.0
50	632.5
51	513.0
52	424.5
53	336.0
54	255.0
55	174.0
56	139.5
57	105.0
58	79.5
59	54.0
60	47.5
61	41.0
62	29.0
63	17.0
64	17.0
65	17.0
66	15.0
67	13.0
68	10.5
69	8.0
70	5.0
71	2.0
72	1.5
73	1.0
74	1.0
75	1.0
76	1.0
77	1.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05642279480910288
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.01880759826970096
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.01880759826970096
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5317.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.56780139176227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.53266331658291	92.1948467180741
2	1.0050251256281406	1.8807598269700958
3	0.1407035175879397	0.39495956366372015
4	0.020100502512562814	0.07523039307880384
5	0.04020100502512563	0.1880759826970096
6	0.06030150753768845	0.33853676885461725
7	0.020100502512562814	0.1316531878879067
8	0.020100502512562814	0.1504607861576077
9	0.0	0.0
>10	0.1407035175879397	3.6298664660522855
>50	0.020100502512562814	1.0156103065638518
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	54	1.0156103065638518	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	43	0.8087267255971413	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	41	0.7711115290577393	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	38	0.7146887342486364	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	24	0.45138235847282304	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	22	0.4137671619334211	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	13	0.24449877750611246	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC	12	0.22569117923641152	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	8	0.1504607861576077	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	7	0.1316531878879067	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT	6	0.11284558961820576	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	6	0.11284558961820576	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	6	0.11284558961820576	RNA PCR Primer, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.018807598269700958
10	0.0	0.0	0.018807598269700958
11	0.0	0.0	0.037615196539401916
12	0.0	0.0	0.05642279480910288
13	0.0	0.0	0.05642279480910288
14	0.0	0.0	0.05642279480910288
15	0.0	0.0	0.07523039307880383
16	0.0	0.0	0.07523039307880383
17	0.0	0.0	0.07523039307880383
18	0.0	0.0	0.07523039307880383
19	0.0	0.0	0.0940379913485048
20	0.0	0.0	0.0940379913485048
21	0.0	0.0	0.11284558961820576
22	0.0	0.0	0.11284558961820576
23	0.0	0.0	0.15046078615760766
24	0.0	0.0	0.22569117923641152
25	0.0	0.0	0.26330637577581345
26	0.0	0.0	0.33853676885461725
27	0.0	0.0	0.45138235847282304
28	0.0	0.0	0.8087267255971412
29	0.0	0.0	1.2601090840699642
30	0.0	0.0	2.1252586044762083
31	0.0	0.0	3.8931728418280986
32	0.0	0.0	5.28493511378597
33	0.0	0.0	6.921196163249953
34	0.0	0.0	8.25653564039872
35	0.0	0.0	9.855181493323302
36	0.0	0.0	11.660710927214595
37	0.0	0.0	14.557081060748542
38	0.0	0.0	17.45345119428249
39	0.0	0.0	21.00808726725597
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	20	6.4162345E-4	45.0	45
GTATCAA	25	0.001923882	36.000004	1
ATGCCGT	25	0.001923882	36.000004	44
TATCAAC	30	0.0047036554	30.000002	2
GTATGCC	35	0.0099890195	25.714285	42
ATCTCGT	35	0.0099890195	25.714285	37
GCCCACG	35	0.0099890195	25.714285	18
CCACGAG	35	0.0099890195	25.714285	20
TATGCCG	35	0.0099890195	25.714285	43
CACGAGA	35	0.0099890195	25.714285	21
CCCACGA	35	0.0099890195	25.714285	19
TCTCGTA	35	0.0099890195	25.714285	38
ACGAGAC	35	0.0099890195	25.714285	22
CGTATGC	35	0.0099890195	25.714285	41
CGAGCCC	35	0.0099890195	25.714285	15
CCGAGCC	35	0.0099890195	25.714285	14
CTCGTAT	35	0.0099890195	25.714285	39
>>END_MODULE