FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_29.341000000076a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_29.341000000076a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5317
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC541.0156103065638518No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC430.8087267255971413No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC410.7711115290577393RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC380.7146887342486364No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC240.45138235847282304RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC220.4137671619334211No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT130.24449877750611246No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC120.22569117923641152No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC80.1504607861576077RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC70.1316531878879067No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT60.11284558961820576No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT60.11284558961820576No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.11284558961820576RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC206.4162345E-445.045
GTATCAA250.00192388236.0000041
ATGCCGT250.00192388236.00000444
TATCAAC300.004703655430.0000022
GTATGCC350.009989019525.71428542
ATCTCGT350.009989019525.71428537
GCCCACG350.009989019525.71428518
CCACGAG350.009989019525.71428520
TATGCCG350.009989019525.71428543
CACGAGA350.009989019525.71428521
CCCACGA350.009989019525.71428519
TCTCGTA350.009989019525.71428538
ACGAGAC350.009989019525.71428522
CGTATGC350.009989019525.71428541
CGAGCCC350.009989019525.71428515
CCGAGCC350.009989019525.71428514
CTCGTAT350.009989019525.71428539
Produced by FastQC (version 0.11.2)