FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_28.34100000007698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_28.34100000007698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152818
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC56063.668416024290332No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT7340.48030991113612276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.2578230313183002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.19304008690075775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6500.039.11538739
TCTCCGA6500.039.11538711
ATCTCGT6550.038.81679537
CCCACGA6500.038.7692319
GACCATC6350.038.62204726
TCTCGTA6600.038.52272838
ATGCCGT6550.038.4732844
AGACCAT6500.038.42307725
AGCCCAC6600.038.18181617
CGAGCCC6600.038.18181615
GACATCT6450.038.02325434
CTCCGAG6700.037.9477612
CACGAGA6700.037.9477621
ACCATCA6350.037.91338727
CCATCAG6350.037.91338728
CATCTCG6550.037.7862636
ACGAGAC6800.037.7205922
TCCGAGC6750.037.66666813
TCGTATG6750.037.66666840
ATCTCCG6700.037.61194210