FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_27.3410000000768b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_27.3410000000768b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995212
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC47890.481204004774862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5900.038.51703343
TGCCGTC6000.038.25008445
CTCGTAT6000.037.50008439
ATCTCGT6150.037.31715837
ATGCCGT6100.037.25418544
CGTATGC6300.036.42682341
TCTCGTA6250.036.3600838
ACGAGAC6350.035.78568322
CATCTCG6100.035.7787736
TCGTATG6550.035.03824640
ATCTCCG6550.034.69473310
TCTCCGA6550.034.69473311
GCCCACG6650.034.5130918
TCCGAGC6700.034.25552713
CTCCGAG6850.033.5054112
CGAGACC6800.033.08664723
GTATGCC7100.032.3223942
CACGAGA7150.031.7816921
CCCACGA7200.031.56098619
CGAGCCC7250.031.34647215
Produced by FastQC (version 0.11.2)