FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_26.3410000000767e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_26.3410000000767e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669083
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC55750.8332299580171667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC7350.038.5706622
CGTATGC7350.038.26454541
TGCCGTC7300.038.2184245
TATGCCG7400.038.00599743
ATGCCGT7400.038.00599744
TCGTATG7550.037.5489240
CTCGTAT7600.037.0058439
TCTCGTA7600.037.0058438
ATCTCCG7650.036.7667210
CGAGACC7700.036.52524623
TCTCCGA7850.036.4032711
GTATGCC7750.036.289642
CGAGCCC7900.035.88535715
CATCTCG7850.035.54069536
CCGAGCC8050.035.49618514
CACGAGA7950.035.3766521
TCCGAGC8050.035.2166913
CTCCGAG8150.035.06065412
ATCTCGT8050.034.9371937
CCACGAG8250.034.63567420