FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_25.34100000007655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_25.34100000007655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732498
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC39750.5426635977163078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5650.033.8489944
ACGAGAC5700.033.55207422
CTCGTAT5650.033.4507739
TCTCGTA5650.033.4507738
TGCCGTC5800.033.3615145
CACGAGA5750.033.26031521
TATGCCG5850.033.07637443
CGAGACC5900.032.79606223
GCCCACG5850.032.69399318
CCACGAG5900.032.4147120
ATCTCCG5950.032.14232310
CGTATGC6050.031.98293741
CCCACGA6100.031.35193619
CCGAGCC6050.031.2412814
CATCTCG6050.031.23914736
TCGTATG6450.031.04599440
CGAGCCC6100.030.98520515
TCTCCGA6200.030.48336411
GGGCGTT300.00514647429.99949811
ATCTCGT6350.029.76328537
Produced by FastQC (version 0.11.2)