FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_23.3410000000763b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_23.3410000000763b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949358
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT751.7025741E-929.9994439
CGAGACG751.7025741E-929.9994423
CGTATGC803.3942342E-928.12447441
TCGTATG1105.456968E-1226.59041240
TATGCCG957.421477E-1026.05214343
ACGAGAC901.1907105E-824.99953322
TAGGACC3900.021.9226674
GTAGGAC7750.021.1931513
GTCCTAC7450.020.2441161
ATGCCGT1251.7667844E-819.7996344
ACGAGAG1151.594035E-719.5658827
TCTCGTA1151.5949263E-719.56485238
AGGACGT6800.019.5216945
TAGGACG6750.019.3329724
TGCCGTC1302.7714123E-819.03810545
TGTAGGA8750.019.0282152
CTGTAGG8200.018.3925191
TCAACGC3350.018.133994
GGACGTG7200.018.124666
CGAGAAA4750.017.99966221
Produced by FastQC (version 0.11.2)