##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_22.3410000000762e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28437 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.30108661251187 38.0 38.0 38.0 32.0 38.0 2 36.289763336498225 38.0 38.0 38.0 32.0 38.0 3 36.27531033512677 38.0 38.0 38.0 32.0 38.0 4 36.38618700988149 38.0 38.0 38.0 32.0 38.0 5 36.386784822590286 38.0 38.0 38.0 32.0 38.0 6 38.28262474944614 40.0 38.0 40.0 38.0 40.0 7 38.160495129584696 40.0 38.0 40.0 38.0 40.0 8 38.12881105601857 40.0 38.0 40.0 38.0 40.0 9 38.193796814009914 40.0 38.0 40.0 38.0 40.0 10 38.10662165488624 40.0 38.0 40.0 38.0 40.0 11 38.099940218729124 40.0 38.0 40.0 38.0 40.0 12 38.0523965256532 40.0 38.0 40.0 32.0 40.0 13 38.08158385202377 40.0 38.0 40.0 32.0 40.0 14 38.01452333227837 40.0 38.0 40.0 32.0 40.0 15 38.036220417062275 40.0 38.0 40.0 32.0 40.0 16 38.06435277982909 40.0 38.0 40.0 32.0 40.0 17 38.00393853078735 40.0 38.0 40.0 32.0 40.0 18 38.03537644617927 40.0 38.0 40.0 32.0 40.0 19 38.06287583078384 40.0 38.0 40.0 32.0 40.0 20 38.05679220733551 40.0 38.0 40.0 32.0 40.0 21 38.062981327144215 40.0 38.0 40.0 32.0 40.0 22 38.03783802792137 40.0 38.0 40.0 32.0 40.0 23 38.02598727010585 40.0 38.0 40.0 32.0 40.0 24 38.0047473362169 40.0 38.0 40.0 32.0 40.0 25 38.056510883707844 40.0 38.0 40.0 32.0 40.0 26 38.013925519569575 40.0 38.0 40.0 32.0 40.0 27 37.91816998980202 40.0 38.0 40.0 32.0 40.0 28 37.92653936772515 40.0 38.0 40.0 32.0 40.0 29 37.93722966557654 40.0 38.0 40.0 32.0 40.0 30 37.89576959594894 40.0 38.0 40.0 32.0 40.0 31 37.8572282589584 40.0 38.0 40.0 32.0 40.0 32 37.863733867848225 40.0 38.0 40.0 32.0 40.0 33 37.839540035868765 40.0 38.0 40.0 32.0 40.0 34 37.79336779547772 40.0 38.0 40.0 32.0 40.0 35 37.807574638674964 40.0 38.0 40.0 32.0 40.0 36 37.7214896086085 40.0 38.0 40.0 32.0 40.0 37 37.68896156415937 40.0 38.0 40.0 32.0 40.0 38 37.7109751380244 40.0 38.0 40.0 32.0 40.0 39 37.605830432183424 40.0 38.0 40.0 32.0 40.0 40 37.56521433343883 40.0 38.0 40.0 32.0 40.0 41 37.5801596511587 40.0 38.0 40.0 32.0 40.0 42 37.51658051130569 40.0 38.0 40.0 32.0 40.0 43 37.46073777121356 40.0 38.0 40.0 32.0 40.0 44 37.37848577557408 40.0 38.0 40.0 32.0 40.0 45 37.24988571227626 40.0 38.0 40.0 32.0 40.0 46 37.12188346168724 40.0 38.0 40.0 32.0 40.0 47 37.081689348384145 40.0 38.0 40.0 32.0 40.0 48 36.996307627386855 40.0 38.0 40.0 32.0 40.0 49 36.9799908569821 40.0 38.0 40.0 32.0 40.0 50 36.95948939761578 40.0 38.0 40.0 32.0 40.0 51 36.47174455814608 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.8729609044817508 2201 2 0.09139877205372926 2201 3 0.11983846217528082 2201 4 0.45411200074477875 2201 5 -0.20867583156813652 2201 6 0.9911490513038927 2201 7 0.14840660238382242 2201 8 0.923576201809766 2201 9 -0.17993769673987003 2201 10 0.8845187148142415 2201 11 0.8826301261527689 2201 12 -0.18217455276777628 2201 13 0.7147316541170383 2201 14 0.43408945653291653 2201 15 -0.6304984110904073 2201 16 -0.12300328020415208 2201 17 0.8341283624273856 2201 18 -0.2817645443861636 2201 19 0.6298336136852498 2201 20 -0.5920927772712119 2201 21 0.5955110163401471 2201 22 0.47943929986065115 2201 23 0.14401383963397762 2201 24 -0.9992703519833128 2201 25 -0.32279320040883874 2201 26 -0.1068368958810737 2201 27 0.08329447913610011 2201 28 0.6860899626200236 2201 29 0.4783001983172568 2201 30 0.5598677889544774 2201 31 1.1769275381621256 2201 32 0.8811281096807448 2201 33 -0.10160574766578634 2201 34 -0.1009892363753977 2201 35 0.3017004874956015 2201 36 -0.5370723579799233 2201 37 0.35185746280034635 2201 38 0.6891853546560327 2201 39 -0.41409074487467024 2201 40 0.11575416878862654 2201 41 0.2778172969119552 2201 42 0.05307327693194708 2201 43 0.19224823280704584 2201 44 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15 0.34386056326857073 2202 16 -0.3024904596913274 2202 17 0.19310272140175044 2202 18 0.8464405838189677 2202 19 0.4759874598390965 2202 20 0.3822661970877661 2202 21 1.2365366573657894 2202 22 0.45379827421962915 2202 23 0.6568343524544886 2202 24 0.923806571093607 2202 25 0.24130936369373046 2202 26 0.5085477195035466 2202 27 -0.5833721875305642 2202 28 0.48096175749181924 2202 29 0.2731719931890524 2202 30 -0.2862860571993693 2202 31 0.5871839484185344 2202 32 0.26574349429613164 2202 33 -0.17852882458885944 2202 34 0.9246517892656314 2202 35 0.04529023108533892 2202 36 -0.28066210156966775 2202 37 -0.0071168961740113446 2202 38 1.0738007392714195 2202 39 0.9448836140996875 2202 40 0.6285746816091446 2202 41 -0.4401314210367602 2202 42 -0.9725677487090749 2202 43 -0.16672612616731186 2202 44 0.47325599632254267 2202 45 -0.3149345849547345 2202 46 -0.9290909199752306 2202 47 -0.6247419033460062 2202 48 -0.834872703114776 2202 49 -0.4974611082872329 2202 50 0.01638788214157927 2202 51 -0.16562941843932322 1102 1 -0.07392554240469451 1102 2 0.25440243505739346 1102 3 0.11251245484927352 1102 4 -0.30046675383397314 1102 5 0.5019468790545716 1102 6 0.26037982053465925 1102 7 -0.3277838738066592 1102 8 -0.10572782749426324 1102 9 -0.042575059377234936 1102 10 0.1720645023600298 1102 11 0.3972821408047835 1102 12 0.7097668391736178 1102 13 -0.7266602872749033 1102 14 0.3278623503058071 1102 15 -0.7715240521160496 1102 16 0.7158245586236873 1102 17 0.46599649429551704 1102 18 -0.1352443978660176 1102 19 0.4631669470185855 1102 20 -0.3558290410074747 1102 21 -0.10595418512505717 1102 22 -0.6011467807254292 1102 23 -0.03181033619020468 1102 24 0.05201169929873828 1102 25 0.12958775197211736 1102 26 -0.2441995332437088 1102 27 0.5558219516635745 1102 28 0.7868225633526222 1102 29 0.5973478173648701 1102 30 0.4096846387713242 1102 31 -0.1527427915082029 1102 32 0.34999257854521915 1102 33 1.4624968164367829 1102 34 0.04186790648174821 1102 35 -0.23492954913443498 1102 36 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-1.1965033064560657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 1.0 17 0.0 18 1.0 19 2.0 20 5.0 21 3.0 22 10.0 23 28.0 24 37.0 25 80.0 26 125.0 27 170.0 28 244.0 29 340.0 30 458.0 31 555.0 32 638.0 33 780.0 34 965.0 35 1247.0 36 1704.0 37 2474.0 38 4482.0 39 14086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.028565397875184 21.16020544571871 13.628368395131218 27.182860761274892 2 13.877263935291015 23.713733075435204 40.77721118340074 21.631791805873043 3 17.48074691423146 27.327073882617714 31.895066286879768 23.297112916271058 4 11.977353447972712 21.081689348384145 38.35496008721033 28.585997116432814 5 12.265710166332596 37.73253156099448 36.63536941308858 13.366388859584344 6 27.97411822625453 42.21964342230193 17.392833280585155 12.413405070858389 7 23.349861096458838 34.314449484826106 22.80128002250589 19.534409396209167 8 22.45665857861237 38.57650244399902 21.788514962900447 17.178324014488165 9 24.18328234342582 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30.23525688363751 30.583394872876884 19.74891866230615 23 19.446495762562858 28.258958399268558 30.57284523683933 21.721700601329257 24 21.535323697999086 28.248408763231005 30.63262650771882 19.583641031051098 25 19.664521574005697 30.604494144952 30.11569434187854 19.615289939163766 26 21.250483524985057 28.04796567851742 30.759222140169495 19.942328656328023 27 18.98231177691036 31.374617575693637 30.22822379294581 19.41484685445019 28 19.151105953511273 28.410169849140203 30.495481239230582 21.943242958117946 29 21.422794246931815 28.8743538347927 30.79790413897387 18.904947779301615 30 19.521029680686453 30.528203685469123 30.10972007314672 19.841046560697706 31 21.514224425923974 28.030382951788162 30.39701796954672 20.058374652741147 32 18.848683053767978 30.836586137778248 30.098111615149275 20.216619193304496 33 20.88124626367057 28.663361114041564 30.562295600801768 19.893097021486092 34 19.460561943946267 28.093680768013503 32.84101698491402 19.60474030312621 35 19.805183387839786 28.202693673734924 32.12364173436016 19.868481204065127 36 22.323029855469986 28.02334986109646 30.277455427787743 19.376164855645815 37 19.71726975419348 30.41108415093013 30.41460069627598 19.457045398600414 38 19.52737630551746 27.647079509090272 32.51397826774976 20.311565917642508 39 19.200337588353204 28.072581495938394 30.03481379892394 22.69226711678447 40 19.516826669479904 27.822906776382883 32.54211063051658 20.118155923620634 41 19.46407848929212 27.0633329816788 30.73108977740268 22.741498751626402 42 21.735766782712663 27.69279459858635 30.186025248795584 20.385413369905407 43 19.506277033442345 26.855856806273515 32.55617681189999 21.081689348384145 44 19.615289939163766 29.437001090129055 30.81197032035728 20.135738650349897 45 19.457045398600414 26.883989169040333 32.49287899567465 21.1660864366846 46 21.78148187220874 27.57674860217322 29.588212540000704 21.05355698561733 47 18.975278686218658 27.900270773991632 29.707775081759678 23.41667545803003 48 19.18275486162394 27.921370046066745 30.256356155712627 22.639518936596687 49 21.457959700390337 27.583781692864928 29.88360234905229 21.074656257692443 50 18.672855786475367 28.156978584238846 32.19748918662306 20.972676442662728 51 18.648895765930508 27.88015192010128 29.525953017302008 23.944999296666197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 198.0 1 121.5 2 45.0 3 44.0 4 43.0 5 36.0 6 29.0 7 32.0 8 35.0 9 45.5 10 56.0 11 73.5 12 91.0 13 172.5 14 254.0 15 309.0 16 364.0 17 324.0 18 284.0 19 253.0 20 222.0 21 218.0 22 214.0 23 231.5 24 249.0 25 316.0 26 470.5 27 558.0 28 813.0 29 1068.0 30 1289.5 31 1511.0 32 1747.5 33 1984.0 34 2025.0 35 2066.0 36 2070.5 37 2075.0 38 2004.0 39 1933.0 40 1829.5 41 1726.0 42 1618.5 43 1511.0 44 1495.5 45 1480.0 46 1414.0 47 1348.0 48 1350.0 49 1352.0 50 1286.5 51 1221.0 52 1490.5 53 1760.0 54 1342.5 55 925.0 56 827.5 57 730.0 58 702.5 59 675.0 60 590.5 61 506.0 62 476.5 63 447.0 64 381.5 65 316.0 66 293.0 67 270.0 68 202.0 69 134.0 70 121.5 71 109.0 72 106.5 73 104.0 74 89.0 75 58.0 76 42.0 77 28.0 78 14.0 79 14.0 80 14.0 81 11.5 82 9.0 83 7.5 84 6.0 85 3.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03868199880437458 2 0.007033090691704469 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0035165453458522347 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0035165453458522347 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0035165453458522347 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0035165453458522347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 28437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.52954840305075 #Duplication Level Percentage of deduplicated Percentage of total 1 89.11304922870985 73.54459709664256 2 8.228823418489897 13.58242161232839 3 1.6102352886051192 3.9867597357370976 4 0.48262947876555573 1.5932477171408432 5 0.17097334786552337 0.7055176594149672 6 0.14743998091687058 0.730089302497425 7 0.07335758431791697 0.4237917813987484 8 0.035790259384648776 0.23630031553944905 9 0.030802091363590037 0.2287874421095936 >10 0.08567149113372743 1.0932330854413985 >50 0.0084911321800026 0.4484923547089084 >100 0.008491132178210008 1.173788584364627 >500 0.004245566089105004 2.2529733126759854 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 643 2.261138657382987 RNA PCR Primer, Index 23 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.6540774343285156 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.523965256531983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 0.24615817420965644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.20395963005942963 No Hit TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT 34 0.11956254175897597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0035165453458522347 10 0.0 0.0 0.0035165453458522347 11 0.0 0.0 0.0035165453458522347 12 0.0 0.0 0.0035165453458522347 13 0.0 0.0 0.0035165453458522347 14 0.0 0.0 0.0035165453458522347 15 0.0 0.0 0.0035165453458522347 16 0.0 0.0 0.0035165453458522347 17 0.0 0.0 0.0035165453458522347 18 0.0 0.0 0.007033090691704469 19 0.0 0.0 0.010549636037556704 20 0.0 0.0 0.010549636037556704 21 0.0 0.0 0.014066181383408939 22 0.0 0.0 0.014066181383408939 23 0.0 0.0 0.017582726729261172 24 0.0 0.0 0.04571508949607905 25 0.0 0.0 0.049231634841931285 26 0.0 0.0 0.052748180187783524 27 0.0 0.0 0.059781270879487994 28 0.0 0.0 0.0808805429546014 29 0.0 0.0 0.11252945106727151 30 0.0 0.0 0.14417835917994162 31 0.0 0.0 0.2602243555930654 32 0.0 0.0 0.404402714773007 33 0.0 0.0 0.5591307099905053 34 0.0 0.0 0.749024158666526 35 0.0 0.0 0.9529837887259556 36 0.0 0.0 1.2026585082814643 37 0.0 0.0 1.5472799521749834 38 0.0 0.0 1.962232302985547 39 0.0 0.0 2.567078102472131 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 80 0.0 42.1875 45 TATGCCG 80 0.0 42.1875 43 ATCTCGT 75 0.0 42.0 37 CGAGACG 75 0.0 42.0 23 CTCGTAT 75 0.0 42.0 39 AGACGAG 60 3.6379788E-12 41.249996 25 TTGATCT 60 3.6379788E-12 41.249996 34 GACGAGA 60 3.6379788E-12 41.249996 26 CGAGAGT 60 3.6379788E-12 41.249996 28 GAGACGA 60 3.6379788E-12 41.249996 24 GTATGCC 85 0.0 39.705883 42 ATCTCCG 85 0.0 39.705883 10 GCCCACG 85 0.0 39.705883 18 CCACGAG 85 0.0 39.705883 20 CATCTCC 85 0.0 39.705883 9 CACGAGA 85 0.0 39.705883 21 CCCACGA 85 0.0 39.705883 19 ACGAGAC 85 0.0 39.705883 22 ATGCCGT 85 0.0 39.705883 44 CGTATGC 85 0.0 39.705883 41 >>END_MODULE