FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_22.3410000000762e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_22.3410000000762e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28437
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC6432.261138657382987RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.6540774343285156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.523965256531983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.24615817420965644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.20395963005942963No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT340.11956254175897597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC800.042.187545
TATGCCG800.042.187543
ATCTCGT750.042.037
CGAGACG750.042.023
CTCGTAT750.042.039
AGACGAG603.6379788E-1241.24999625
TTGATCT603.6379788E-1241.24999634
GACGAGA603.6379788E-1241.24999626
CGAGAGT603.6379788E-1241.24999628
GAGACGA603.6379788E-1241.24999624
GTATGCC850.039.70588342
ATCTCCG850.039.70588310
GCCCACG850.039.70588318
CCACGAG850.039.70588320
CATCTCC850.039.7058839
CACGAGA850.039.70588321
CCCACGA850.039.70588319
ACGAGAC850.039.70588322
ATGCCGT850.039.70588344
CGTATGC850.039.70588341