Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 0h_22.3410000000762e.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 28437 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC | 643 | 2.261138657382987 | RNA PCR Primer, Index 23 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 186 | 0.6540774343285156 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 149 | 0.523965256531983 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 70 | 0.24615817420965644 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 58 | 0.20395963005942963 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT | 34 | 0.11956254175897597 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 80 | 0.0 | 42.1875 | 45 |
TATGCCG | 80 | 0.0 | 42.1875 | 43 |
ATCTCGT | 75 | 0.0 | 42.0 | 37 |
CGAGACG | 75 | 0.0 | 42.0 | 23 |
CTCGTAT | 75 | 0.0 | 42.0 | 39 |
AGACGAG | 60 | 3.6379788E-12 | 41.249996 | 25 |
TTGATCT | 60 | 3.6379788E-12 | 41.249996 | 34 |
GACGAGA | 60 | 3.6379788E-12 | 41.249996 | 26 |
CGAGAGT | 60 | 3.6379788E-12 | 41.249996 | 28 |
GAGACGA | 60 | 3.6379788E-12 | 41.249996 | 24 |
GTATGCC | 85 | 0.0 | 39.705883 | 42 |
ATCTCCG | 85 | 0.0 | 39.705883 | 10 |
GCCCACG | 85 | 0.0 | 39.705883 | 18 |
CCACGAG | 85 | 0.0 | 39.705883 | 20 |
CATCTCC | 85 | 0.0 | 39.705883 | 9 |
CACGAGA | 85 | 0.0 | 39.705883 | 21 |
CCCACGA | 85 | 0.0 | 39.705883 | 19 |
ACGAGAC | 85 | 0.0 | 39.705883 | 22 |
ATGCCGT | 85 | 0.0 | 39.705883 | 44 |
CGTATGC | 85 | 0.0 | 39.705883 | 41 |