FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_21.34100000007611.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_21.34100000007611.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677960
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1300.039.80736523
TATGCCG1400.038.57111443
CGAGCCC1600.035.1559615
ATGCCGT1600.033.7497244
CGTATGC1700.033.08796341
CGAGAGT1500.032.9997328
CCGAGCC1750.032.14259314
CTCGTAT1700.031.76444439
TGCCGTC1700.031.76444445
TCGTATG1800.031.24974340
TCTCCGA1750.030.8568911
ACGAGAC1750.030.8568922
ATCTCCG1850.030.40515510
ATCTCGT1800.029.99975437
GATCTCG1600.029.53100636
GAGACGA1700.029.11740724
CACGAGA1900.028.4208221
GACGAGA1700.027.7938926
CTCCGAG1950.027.69208312
TCTCGTA1950.027.69208338
Produced by FastQC (version 0.11.2)