FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_20.34100000007604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_20.34100000007604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC191.2402088772845952RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC150.97911227154047No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC130.8485639686684074No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC110.7180156657963447No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC70.45691906005221933No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC60.39164490861618795RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC60.39164490861618795No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC50.3263707571801566No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC50.3263707571801566No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT30.19582245430809397No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT20.13054830287206268No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT20.13054830287206268No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.13054830287206268No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC20.13054830287206268No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC20.13054830287206268No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)