FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_2.341000000074c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_2.341000000074c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263863
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC22680.8595369566782761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.12051708651838265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2600.036.34538743
TGCCGTC2550.036.1757145
ACGAGAC2700.035.83257722
CTCGTAT2650.035.65962639
TCTCGTA2650.035.65962638
CGAGACC2700.034.9992623
TATCTCG2650.034.8105936
ATGCCGT2650.034.8105944
CACGAGA2800.034.5528421
CGTATGC2750.034.3629141
TCGTATG2750.034.3629140
ATATCTC2750.033.54474635
GAATATC2700.033.3326333
CCCACGA2850.033.15719619
GTATGCC2850.033.15719642
GACCCTA2800.032.94573226
ATCTCCG2900.032.5855210
CGAGCCC2900.032.5855215
AATATCT2850.032.3677434
TAGAATA2800.032.14217831
Produced by FastQC (version 0.11.2)