FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_19.341000000075f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_19.341000000075f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748219
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC11150.14902054077749963RNA PCR Primer, Index 23 (95% over 21bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT9490.12683452304739656No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA9010.12041928900495712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCAC250.002106808635.9994138
CGTATGC1400.035.35656741
TGCCGTC1500.034.49943545
TCGTATG1600.033.74944740
CCCACGA1550.031.93496119
ACGAGAC1600.030.93699322
CACGAGA1700.030.4406821
TATGCCG1650.029.99951243
ACGAGAG1600.029.5327427
AATCCCG1001.8189894E-1229.24952119
GCCCACG1700.029.1171718
GAGACGA1750.028.28714224
CTCGTAT1750.028.28525239
ATGCCGT1750.028.28525244
GTGCGGG406.155074E-428.132061
CGAGACG1800.027.49955223
TCTCGTA1850.026.75631938
CCACGAG1900.026.05220620
CGAGCCC2000.025.87457715
CGAGAGT1850.025.5418328
Produced by FastQC (version 0.11.2)