FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_18.341000000075e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_18.341000000075e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708574
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC9750.13760030709565974RNA PCR Primer, Index 23 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG850.034.41036223
TGCCGTC950.033.1565445
TATGCCG900.032.49867643
CTCGTAT855.456968E-1231.7634139
ACGAGAC1001.8189894E-1229.24880822
TCTCGTA1004.5474735E-1126.998938
ATCTCCG1159.094947E-1225.43374610
ATGCCGT1159.094947E-1225.43374644
GTCCTAC7950.024.6390251
GAGACGA1101.5097612E-1024.54791824
CGAGAGT1101.5097612E-1024.54791828
CGTATGC1201.6370905E-1124.37400641
CGAGCCC1201.6370905E-1124.37400615
TCTCCGA1252.910383E-1123.39904411
CCACGAG1353.6379788E-1223.33238220
TCGTATG1204.5110937E-1022.49908440
CACGAGA1305.0931703E-1122.49908321
TCCTACA9150.021.6415182
TCCGAGC1501.4551915E-1120.99914413
GATCTCG1401.364242E-1020.89200636
Produced by FastQC (version 0.11.2)