FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_17.341000000075cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_17.341000000075cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110864
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12991.1717058738634722No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC7130.6431303218357627RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.574577861163227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.33915427911675566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.23361956992350988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.15153701832876315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT900.044.9986539
TATGCCG900.044.9986543
GAGACGA900.044.9986524
TCTCGTA900.044.9986538
GACGAGA900.044.9986526
ACGAGAC900.044.9986522
TGCCGTC950.042.64953245
GCCCACG950.042.63029518
CCCACGA950.042.63029519
AGACGAG950.042.63029525
TCGTATG950.042.63029540
CGAGACG950.042.63029523
CGTATGC1000.040.4987841
GATCTCG950.040.26194836
ATCTCGT1050.038.57026737
TCCGAGC1050.038.57026713
ACGAGAG1050.038.57026727
CCACGAG1050.038.57026720
GTTGATC1000.038.24884833
AGCCCAC1100.036.81707417