FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_15.341000000075a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_15.341000000075a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669170
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT750.038.9993539
TATGCCG950.030.78896343
AATCTCG950.030.78896336
TCGTATG950.030.78896340
GACGTTC852.0372681E-1029.11716326
CGAGACG852.0372681E-1029.11716323
ATGCCGT1053.6379788E-1227.8566844
TGCCGTC1053.6379788E-1227.8566845
GTATCAA4550.027.203451
CGTATGC1159.094947E-1225.4343641
TCTCGTA1159.094947E-1225.4343638
AGACGTT1052.3628672E-923.57103725
GAGACGT1052.3628672E-923.57103724
ACGAGAC1252.910383E-1123.3996122
ATCTCCG1305.0931703E-1122.50130810
CCACGAG1305.0931703E-1122.49962620
TCAACGC5100.022.4996264
TCTCCGA1358.367351E-1121.66792511
TCCGAGC1358.367351E-1121.66630613
ACGTTCA1156.7575456E-921.52138127
Produced by FastQC (version 0.11.2)