FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_14.34100000007599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_14.34100000007599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685685
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC8890.12965137052728293RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1200.033.7494541
ATGCCGT1250.030.59950344
ACGAGAC1350.029.99951422
TGCCGTC1300.029.42474645
TATGCCG1300.029.422643
AATCTCG1300.029.422636
GACGTTC1350.028.33287426
CGAGACG1400.027.32098623
CGTTCAT1600.026.71831728
CTCGTAT1450.026.37888339
GTATCAA4250.025.4261861
TCGTATG1600.025.3120940
ACGTTCA1800.023.74961527
AGACGTT1650.023.18144225
CGAGCCC1750.023.14248315
ATCTCCG1700.022.49963610
CACGAGA1850.021.89153721
TCTCGTA1750.021.85678938
TCTCCGA1750.021.85678911
TCAACGC4800.021.562154
Produced by FastQC (version 0.11.2)