FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_13.3410000000758c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_13.3410000000758c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560969
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC15980.28486422600892386RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2400.031.87960845
CGAGACG2400.030.93645923
ATCCGCT300.005143644330.00166112
TATGCCG2550.029.99898743
ATGCCGT2550.029.99898744
CGTATGC2600.029.42208741
GAGACGT2450.029.38676524
GACGTTC2550.029.11666526
CGTTCAT2600.028.5567328
CCACGAG2600.028.5567320
ATCTCGT2700.028.33237837
CTCCGAG2800.028.12655812
CCGAGCC2800.028.12655814
TCTCGTA2750.027.81724538
ACGAGAC2750.027.81724522
CGAGCCC2850.027.63311215
CCCACGA2700.027.49907319
AGACGTT2700.027.49907325
TCCGAGC2950.027.4591513
GCCCACG2800.027.32050918
Produced by FastQC (version 0.11.2)