FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_12.3410000000757f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_12.3410000000757f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287156
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC12140.42276671913524355RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1450.037.24065839
TCGTATG1500.035.99930240
CGTTCAT1450.035.68896528
GAGACGT1450.035.68896524
TCTCGTA1600.035.15556738
ACGAGAC1550.034.83803622
CGTATGC1500.034.49933241
TATGCCG1500.034.49933243
GCCCACG1600.033.74934418
CCCACGA1600.033.74934419
CGAGCCC1600.033.74934415
CGAGACG1600.033.74934423
ACGTTCA1550.033.3864527
ATGCCGT1550.033.3864544
ATCTCGT1650.032.72664337
CCGAGCC1650.032.72664314
GACGTTC1600.032.34312426
AGACGTT1600.032.34312425
TGCCGTC1600.032.34312445
TCCGAGC1750.030.85654613
Produced by FastQC (version 0.11.2)