FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_11.34100000007562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_11.34100000007562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126548
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2050.16199386793943799No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA2030.16041343995954105No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1700.13433637829124126No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1430.11300060056263236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG253.871487E-544.9992118
GAGACGT253.871487E-544.9992124
TCTCGTA253.871487E-544.9992138
CGAGACG253.871487E-544.9992123
CCGAGCC356.205062E-638.5707514
ATCTCCG301.134306E-437.4993410
GTATGCC301.134306E-437.4993442
TCTCCGA301.134306E-437.4993411
TGCCGTC301.134306E-437.4993445
ACGTTCA401.549843E-533.74940527
CGAGCCC401.549843E-533.74940515
CGTATGC352.8065906E-432.14229241
CTCGTAT352.8065906E-432.14229239
CACGAGA352.8065906E-432.14229221
TATGCCG352.8065906E-432.14229243
CGTTCAT352.8065906E-432.14229228
AGACGTT352.8065906E-432.14229225
TCGTATG352.8065906E-432.14229240
ATGCCGT352.8065906E-432.14229244
ACGAGAC352.8065906E-432.14229222
Produced by FastQC (version 0.11.2)