FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_10.34100000007556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_10.34100000007556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641365
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC12870.20066576754266294RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTC1600.035.1581426
CTCGTAT1550.034.83786439
CGAGACG1550.034.83786423
ACGTTCA1650.034.0927427
TATGCCG1600.033.7491843
TCTCGTA1650.032.7264838
CGTATGC1750.032.1420841
CGTTCAT1700.031.76641328
CACGAGA1700.031.76393521
CCCACGA1800.031.24924319
AATCTCG1750.030.85639636
CGAGCCC1800.029.99927315
TGCCGTC1800.029.99927345
ATCTCCG1850.029.18848210
TCGTATG1850.029.18848240
ATGCCGT1850.029.18848244
CCGAGCC1950.027.69163514
ACGAGAC2000.026.99934622
GCCCACG2200.026.59026318
ATCTCGT2050.026.34082637
Produced by FastQC (version 0.11.2)