##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_1.341000000074a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23562 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3148714031067 38.0 38.0 38.0 32.0 38.0 2 36.36321195144725 38.0 38.0 38.0 32.0 38.0 3 36.32556659027247 38.0 38.0 38.0 32.0 38.0 4 36.41800356506239 38.0 38.0 38.0 32.0 38.0 5 36.462779051014344 38.0 38.0 38.0 32.0 38.0 6 38.39330277565572 40.0 38.0 40.0 38.0 40.0 7 38.249978779390545 40.0 38.0 40.0 38.0 40.0 8 38.243188184364655 40.0 38.0 40.0 38.0 40.0 9 38.18037518037518 40.0 38.0 40.0 38.0 40.0 10 38.09247941600883 40.0 38.0 40.0 32.0 40.0 11 38.09655377302436 40.0 38.0 40.0 32.0 40.0 12 37.934682964094726 40.0 38.0 40.0 32.0 40.0 13 37.9572192513369 40.0 38.0 40.0 32.0 40.0 14 37.896316102198455 40.0 38.0 40.0 32.0 40.0 15 37.953654188948306 40.0 38.0 40.0 32.0 40.0 16 37.924369747899156 40.0 38.0 40.0 32.0 40.0 17 37.89372718784483 40.0 38.0 40.0 32.0 40.0 18 37.96197266785502 40.0 38.0 40.0 32.0 40.0 19 37.95072574484339 40.0 38.0 40.0 32.0 40.0 20 38.00025464731347 40.0 38.0 40.0 32.0 40.0 21 37.99129955012308 40.0 38.0 40.0 32.0 40.0 22 37.995161701044054 40.0 38.0 40.0 32.0 40.0 23 37.908072319837025 40.0 38.0 40.0 32.0 40.0 24 37.89232662762075 40.0 38.0 40.0 32.0 40.0 25 37.795772854596386 40.0 38.0 40.0 32.0 40.0 26 37.75299210593328 40.0 38.0 40.0 32.0 40.0 27 37.693447075800016 40.0 38.0 40.0 32.0 40.0 28 37.63542992954758 40.0 38.0 40.0 32.0 40.0 29 37.51948051948052 40.0 38.0 40.0 32.0 40.0 30 37.55835667600373 40.0 38.0 40.0 32.0 40.0 31 37.528775146422205 40.0 38.0 40.0 32.0 40.0 32 37.504838298955946 40.0 38.0 40.0 32.0 40.0 33 37.36970545794075 40.0 38.0 40.0 32.0 40.0 34 37.34377387318564 40.0 38.0 40.0 32.0 40.0 35 37.36155674390969 40.0 38.0 40.0 32.0 40.0 36 37.25006366182837 40.0 38.0 40.0 32.0 40.0 37 37.235803412274 40.0 38.0 40.0 32.0 40.0 38 37.160003395297515 40.0 38.0 40.0 32.0 40.0 39 37.03463203463203 40.0 38.0 40.0 32.0 40.0 40 36.97568118156354 40.0 38.0 40.0 32.0 40.0 41 36.84483490365843 40.0 38.0 40.0 32.0 40.0 42 36.75918852389441 40.0 38.0 40.0 32.0 40.0 43 36.66611493082081 40.0 38.0 40.0 27.0 40.0 44 36.53170359052712 40.0 38.0 40.0 27.0 40.0 45 36.375901875901874 40.0 38.0 40.0 27.0 40.0 46 36.17328749681691 40.0 38.0 40.0 27.0 40.0 47 36.02105084458026 40.0 38.0 40.0 27.0 40.0 48 35.85790679908327 40.0 38.0 40.0 27.0 40.0 49 35.85018249724132 40.0 38.0 40.0 27.0 40.0 50 35.89415160003395 40.0 38.0 40.0 27.0 40.0 51 35.702359731771494 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.04893804727089446 2201 2 0.6003620453611589 2201 3 -0.5666171180109032 2201 4 0.7564123495920612 2201 5 0.30369575763716483 2201 6 0.14647226641037037 2201 7 1.2342518742436184 2201 8 0.31385213251753186 2201 9 -0.5059979680175104 2201 10 -0.11406630522758832 2201 11 0.3853503121366728 2201 12 -1.0355910267021073 2201 13 0.6148719473959616 2201 14 0.5515515823379431 2201 15 0.5671798079959487 2201 16 0.38378757423744503 2201 17 -0.04490585432370864 2201 18 -0.42937006517364296 2201 19 0.022925663703823318 2201 20 0.664859070083061 2201 21 1.579461820618242 2201 22 1.4865771734726323 2201 23 0.04073296886496536 2201 24 -0.8211752343900969 2201 25 -1.3120750500009848 2201 26 0.33974045586409574 2201 27 0.7797732561066013 2201 28 1.092069344859624 2201 29 0.3347156939277198 2201 30 1.1163262259091766 2201 31 0.07073834243337274 2201 32 0.9610853881292201 2201 33 0.3402798793145507 2201 34 -0.03271469210218925 2201 35 1.8331051638044826 2201 36 1.5790552063895262 2201 37 0.8171595524332531 2201 38 0.5161545887100374 2201 39 0.8329757047282342 2201 40 1.3782301991563983 2201 41 0.4014877552372269 2201 42 0.5027617224776577 2201 43 1.547416632580216 2201 44 -0.47494492872624505 2201 45 0.5910464270155913 2201 46 1.4171428081737005 2201 47 1.5827810046963293 2201 48 2.396181410381601 2201 49 1.5572913606812904 2201 50 2.228254856758781 2201 51 1.7760445751801583 1101 1 1.0193731481750277 1101 2 0.3204758973535746 1101 3 -0.5495393191494244 1101 4 0.0894294273909253 1101 5 0.6291227026086972 1101 6 0.7745557578714752 1101 7 1.0444985535605085 1101 8 0.888804694187364 1101 9 0.907664271071674 1101 10 -0.14632436974371643 1101 11 0.6955969914535629 1101 12 1.355300813905103 1101 13 1.5370730859158854 1101 14 1.3674908612753214 1101 15 1.500766145756856 1101 16 1.2319849176909514 1101 17 1.1571814322038065 1101 18 1.3808766141432471 1101 19 1.1140072576317195 1101 20 1.3992044211267043 1101 21 0.5310747238440499 1101 22 0.9970136061102082 1101 23 0.7228961567207506 1101 24 1.2262630957806806 1101 25 1.2306194471527192 1101 26 0.5664956740804143 1101 27 0.2105132940572645 1101 28 0.6850484719943495 1101 29 1.2047346692597927 1101 30 0.3478252771425687 1101 31 0.2870571280121226 1101 32 1.2182011376548374 1101 33 0.4655170709654044 1101 34 -0.020380726257791082 1101 35 0.3511317292693761 1101 36 -0.05661841789889621 1101 37 1.4642183759626661 1101 38 1.0095132224861274 1101 39 0.4212109988458792 1101 40 1.2207349429894165 1101 41 -0.02450844969636279 1101 42 0.00371048908107241 1101 43 0.6394469931115268 1101 44 0.14270213009728394 1101 45 0.09958532644633067 1101 46 -1.0752670589989748 1101 47 -1.2065928093454161 1101 48 -1.3533442063166916 1101 49 -1.2235435539297157 1101 50 -0.19204874855430631 1101 51 -0.6101034324099786 2202 1 0.6343464102071081 2202 2 -0.9050142987248577 2202 3 0.020870663025263525 2202 4 -0.13606076868750705 2202 5 0.05247386125397213 2202 6 0.1738427453937561 2202 7 0.14432030044107336 2202 8 -0.533655198860771 2202 9 -0.48449259167342973 2202 10 0.27791805449870566 2202 11 -0.46215701924163 2202 12 -0.8782107725476607 2202 13 -0.08503030089337926 2202 14 0.0412876527191699 2202 15 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2202 51 1.157276246734412 1102 1 0.48996138346914364 1102 2 0.37929942676533557 1102 3 -0.3730687309141274 1102 4 0.5011941332732732 1102 5 -1.7826420032736507 1102 6 -0.6960324774226478 1102 7 -0.13197203467478857 1102 8 -0.4053129528714621 1102 9 -0.18057102304597805 1102 10 0.7360285714327546 1102 11 0.48971463851238184 1102 12 0.767065519787451 1102 13 -0.4041033846723465 1102 14 0.7792555671576764 1102 15 -0.49923385424314404 1102 16 -0.7091915528972805 1102 17 -0.6075244501491355 1102 18 -1.060299856444992 1102 19 -0.9742280364859326 1102 20 -0.07138381416741879 1102 21 -0.49833704086183417 1102 22 -0.17945698212508887 1102 23 -0.9241626668086624 1102 24 -0.7149133748075585 1102 25 -0.35761584696493287 1102 26 -0.22762197297840459 1102 27 -0.6718396471192065 1102 28 -1.3149515280056505 1102 29 -0.059971213093149345 1102 30 -1.2109982522691922 1102 31 0.6694100691885936 1102 32 0.27702466706659834 1102 33 -0.32860057609341453 1102 34 -1.432145432140139 1102 35 -1.590044741318863 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0.6371353299046589 2203 22 1.0897052282135888 2203 23 0.04464303729115926 2203 24 0.8376712954241725 2203 25 0.4445231904682245 2203 26 -0.30248828313883536 2203 27 0.8130088377292779 2203 28 -1.3996217597346998 2203 29 -0.7141601613997466 2203 30 0.3157397156452433 2203 31 0.21932094262887603 2203 32 0.9659729736619624 2203 33 -0.937334978945465 2203 34 -0.5595964125323007 2203 35 -1.6595634578964038 2203 36 -1.0191852628186808 2203 37 -1.0616283263546222 2203 38 0.04694637756634279 2203 39 -0.01550914375661705 2203 40 0.2599506292639262 2203 41 0.6947428872020325 2203 42 -0.062243165107872755 2203 43 0.9540637489047512 2203 44 -0.0203994741807918 2203 45 0.566608499351851 2203 46 0.10922491961065361 2203 47 0.8183626273747322 2203 48 0.9015577544676177 2203 49 1.3138896011504997 2203 50 2.22238975411949 2203 51 1.5209126103707717 1103 1 0.7252555011162016 1103 2 0.9283190346084709 1103 3 0.26582015797475833 1103 4 0.5306058979791572 1103 5 0.13075668953680264 1103 6 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1215 48 -2.3177404911464166 1215 49 -2.0377850399978286 1215 50 -2.973782494684343 1215 51 -1.170474949437839 1212 1 0.8278196036803038 1212 2 -0.8280912218017846 1212 3 0.7038543460089528 1212 4 -0.34118897381571145 1212 5 0.1200729288530411 1212 6 0.008341579892586992 1212 7 -0.30090114478036867 1212 8 0.2915196263140558 1212 9 -0.20244130962214513 1212 10 0.05880383538448797 1212 11 -0.09852065560526313 1212 12 0.2919523976155105 1212 13 0.27161305574997385 1212 14 0.05527366670518319 1212 15 -0.8778160563547601 1212 16 0.22746003081312693 1212 17 -0.6520191711144463 1212 18 0.548297428622881 1212 19 -0.30379063075440627 1212 20 -0.679226951422315 1212 21 -0.7125150197456946 1212 22 -0.5489894104810489 1212 23 -0.7409047482566251 1212 24 0.5369719947248726 1212 25 0.7102574562024913 1212 26 0.06347908282852899 1212 27 -1.4667114419910021 1212 28 -1.0336543937673355 1212 29 -0.16404361128319778 1212 30 -0.6702742703687434 1212 31 -0.5032897799818485 1212 32 -0.22750021981122615 1212 33 -0.5224165640270542 1212 34 0.5942497413138526 1212 35 -0.06515786349080344 1212 36 -0.0867843304177498 1212 37 -1.9007891655154623 1212 38 -1.3027039720840108 1212 39 -0.44907557732305037 1212 40 -0.0221006527873584 1212 41 -0.9905717981126543 1212 42 0.05197594911123815 1212 43 0.43658123142223815 1212 44 0.8840294302481126 1212 45 0.7647436974870558 1212 46 0.11854892893466484 1212 47 0.3801341891462897 1212 48 0.54258339549326 1212 49 0.2719315591924598 1212 50 -1.2531347214049902 1212 51 -0.2669661775080172 1213 1 -0.18383540797470488 1213 2 -2.21562035933092 1213 3 -0.8730687309141274 1213 4 -1.5148486474753895 1213 5 -0.6823746235945123 1213 6 -1.288278466727455 1213 7 -1.7950736389528643 1213 8 -1.3063824715880372 1213 9 -1.8102501674310005 1213 10 -1.23723346065281 1213 11 -0.4394297465143566 1213 12 -1.5069986513355431 1213 13 -0.8880606039236838 1213 14 -1.3223487109171899 1213 15 -1.9430841216228245 1213 16 -0.09421829086520006 1213 17 -0.3227650918603686 1213 18 -0.7768773965519458 1213 19 -1.3766344535982284 1213 20 -0.26256028475565074 1213 21 -3.014379821610497 1213 22 -1.8572644687561066 1213 23 -1.1674781748300518 1213 24 -0.45020749245461644 1213 25 0.17179591774095115 1213 26 -1.6433973740479217 1213 27 -1.8082032834828397 1213 28 -1.2935611536740979 1213 29 -1.0626450098845979 1213 30 -1.0706239207183899 1213 31 -1.6821942088862727 1213 32 -0.7991785414895531 1213 33 -1.263092554703043 1213 34 -0.9308085337444183 1213 35 -1.5004725488054902 1213 36 0.16263291899949905 1213 37 -2.56920408393038 1213 38 -1.566689986070024 1213 39 -0.8942970225444924 1213 40 -2.3915645222512296 1213 41 -2.032529840070694 1213 42 -2.2061825590472637 1213 43 -1.9929059480649443 1213 44 -1.8613085650898782 1213 45 -0.5621793794360244 1213 46 0.631952192337927 1213 47 -1.8255767665646658 1213 48 -3.4469270940172265 1213 49 -2.345201307940407 1213 50 -2.5503375186077832 1213 51 -2.7290873896292283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 0.0 17 2.0 18 1.0 19 6.0 20 12.0 21 16.0 22 23.0 23 58.0 24 91.0 25 119.0 26 161.0 27 197.0 28 305.0 29 340.0 30 417.0 31 512.0 32 597.0 33 710.0 34 901.0 35 1044.0 36 1374.0 37 2090.0 38 3580.0 39 11005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.7001146058831 29.381552697482917 13.625366102126577 22.29296659450741 2 12.626262626262626 32.166199813258636 37.870299635005516 17.33723792547322 3 14.132925897631779 38.1376793141499 29.717341482047367 18.01205330617095 4 9.990662931839402 34.712672947967064 29.772515066632714 25.52414905356082 5 10.058568882098294 43.73567608861727 30.21814786520669 15.987607164077753 6 20.23172905525847 52.3342670401494 12.774806892453952 14.65919701213819 7 17.901706137000254 44.37229437229438 17.0995670995671 20.626432391138273 8 19.866734572616927 44.81368304897717 15.699006875477464 19.620575502928446 9 20.567014684661743 27.574059927001105 17.28630846277905 34.5726169255581 10 13.878278584160936 41.50326797385621 22.790934555640437 21.827518886342414 11 24.005772250753363 36.186919061160395 23.52616612198124 16.281142566105004 12 20.1807995925643 35.4978354978355 23.58458534929123 20.73677956030897 13 22.773958068075714 33.409727527374585 27.78626602156014 16.03004838298956 14 18.377047788812494 31.43196672608437 25.413801884390118 24.777183600713013 15 18.427977251506665 34.93761140819964 25.689669807316868 20.944741532976828 16 19.624819624819626 38.72761225702403 24.174518292165352 17.473049825991 17 14.078349815372862 39.49323033827088 26.391069988540384 20.03734985781588 18 18.57730996137685 31.361147659267434 32.77025593141208 17.291286447943634 19 14.200831847890672 31.22400475341652 34.89517018928784 19.679993209404977 20 13.907987437399202 30.863254392666157 34.72116119174943 20.507596978185212 21 13.449622273151684 31.690858161446396 34.890926067396656 19.968593498005262 22 13.589678295560647 36.58433070197776 34.86546133604957 14.96052966641202 23 14.480943892708598 30.99906629318394 35.132841015193954 19.387148798913508 24 19.149477973007382 31.89457601222307 34.84848484848485 14.107461166284697 25 14.722858840505898 36.45700704524234 35.132841015193954 13.687293099057804 26 19.28104575163399 31.966726084373143 34.72964943553179 14.022578728461083 27 13.517528223410578 36.3763687293099 35.1455733808675 14.96052966641202 28 13.619387148798914 31.028775146422205 34.470758000169766 20.881079704609114 29 13.17375435022494 31.427722604193193 34.89517018928784 20.503352856294033 30 13.398692810457517 30.973601561836855 35.48510313216195 20.14260249554367 31 14.12019353195824 31.78422884305237 39.79713097360156 14.298446651387827 32 13.84008148714031 36.91112808759868 35.26440879382056 13.984381631440455 33 19.25133689839572 31.89457601222307 34.9248790425261 13.929208046855104 34 13.95891690009337 37.05967235379 35.06917918682625 13.912231559290383 35 13.428401663695782 37.41617859264918 35.35777947542653 13.797640268228504 36 14.048043459808165 32.340208810797044 39.517018928783635 14.094728800611152 37 13.704269586622528 36.97478991596639 34.9248790425261 14.396061454884984 38 13.66182836771072 31.847890671420082 39.78015448603684 14.710126474832357 39 14.031066972243442 31.40650199473729 34.22884305237247 20.333587980646804 40 14.090484678719973 31.071216365334013 40.085731262201854 14.752567693744165 41 14.73134708428826 31.512605042016805 33.39699516170104 20.35905271199389 42 19.552669552669553 31.491384432560903 34.31372549019608 14.642220524573466 43 14.209320091673034 32.43357949240302 38.32442067736185 15.032679738562091 44 13.831593243357949 38.06977336389102 33.45216874628639 14.646464646464647 45 14.833206009676598 32.31898820134114 37.271878448349035 15.575927340633221 46 20.24446142093201 32.88345641286818 31.25371360665478 15.61836855954503 47 14.222052457346576 33.32484508955097 31.126389949919364 21.32671250318309 48 14.888379594261947 34.04210168916052 29.76827094474153 21.301247771836007 49 20.12987012987013 33.98692810457516 30.544945250827602 15.338256514727103 50 14.281470163823103 34.3094813683049 35.319582378405904 16.08946608946609 51 14.057128305250203 34.875429735579985 30.019948219515303 21.047493739654513 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3269.0 1 1802.0 2 335.0 3 310.5 4 286.0 5 283.5 6 281.0 7 195.5 8 110.0 9 171.0 10 232.0 11 362.0 12 492.0 13 810.5 14 1129.0 15 1278.0 16 1427.0 17 1077.5 18 728.0 19 527.0 20 326.0 21 284.5 22 243.0 23 237.0 24 231.0 25 220.5 26 225.0 27 240.0 28 292.5 29 345.0 30 373.0 31 401.0 32 411.5 33 422.0 34 420.5 35 419.0 36 456.5 37 494.0 38 483.5 39 473.0 40 493.0 41 513.0 42 508.0 43 503.0 44 545.5 45 588.0 46 606.5 47 625.0 48 776.0 49 927.0 50 1515.0 51 2103.0 52 1450.5 53 798.0 54 820.5 55 843.0 56 853.0 57 863.0 58 855.5 59 848.0 60 813.5 61 779.0 62 698.0 63 617.0 64 558.0 65 499.0 66 419.5 67 340.0 68 303.0 69 266.0 70 218.5 71 171.0 72 138.0 73 105.0 74 73.0 75 34.0 76 27.0 77 18.5 78 10.0 79 6.0 80 2.0 81 1.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012732365673542143 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.004244121891180715 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.004244121891180715 18 0.004244121891180715 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.004244121891180715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 23562.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.29335452895255 #Duplication Level Percentage of deduplicated Percentage of total 1 91.60815959492922 54.31755084607004 2 6.146332356606887 7.288733269461291 3 1.1026271933809757 1.9613539527120634 4 0.38041339954136666 0.9022394626628131 5 0.19668641100119344 0.5831098549260517 6 0.09370973440920322 0.3333818703083322 7 0.07174477102739223 0.29777917008879834 8 0.04295531983775312 0.20375720064355557 9 0.03576170385647457 0.19083882467892205 >10 0.22870278981554568 3.2247976426297718 >50 0.021440013598615457 0.9237752379166763 >100 0.04288002719723091 5.034151296536749 >500 0.014293342399076972 6.093526569376975 >1k 0.014293342399076972 18.64500480198796 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3114 13.216195569136746 No Hit CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC 1286 5.4579407520583985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 3.9640098463627877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 2.13903743315508 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 1.5575927340633222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 1.0101010101010102 No Hit TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT 164 0.6960359901536372 RNA PCR Primer, Index 44 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.6153976742212036 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.5941770647653001 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.5687123334182157 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.38197097020626436 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.3225532637297343 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52 0.22069433834139718 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.19947372888549358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA 45 0.19098548510313218 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.18674136321195145 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 42 0.17825311942959002 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA 40 0.16976487564722859 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.15703250997368645 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.14854426619132502 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.14854426619132502 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA 34 0.1443001443001443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA 34 0.1443001443001443 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT 28 0.11883541295306002 RNA PCR Primer, Index 44 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA 25 0.10610304727951786 No Hit ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC 24 0.10185892538833714 RNA PCR Primer, Index 37 (95% over 23bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.004244121891180714 22 0.0 0.0 0.02546473134708429 23 0.0 0.0 0.04668534080298786 24 0.0 0.0 0.08063831593243358 25 0.0 0.0 0.09337068160597572 26 0.0 0.0 0.1443001443001443 27 0.0 0.0 0.15703250997368645 28 0.0 0.0 0.16976487564722859 29 0.0 0.0 0.22918258212375858 30 0.0 0.0 0.2504031915796622 31 0.0 0.0 0.3183091418385536 32 0.0 0.0 0.39894745777098717 33 0.0 0.0 0.4668534080298786 34 0.0 0.0 0.5092946269416858 35 0.0 0.0 0.6153976742212036 36 0.0 0.0 0.7002801120448179 37 0.0 0.0 0.8742891095832273 38 0.0 0.0 1.0355657414480943 39 0.0 0.0 1.2307953484424072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 150 0.0 42.000004 1 GGTATCA 90 0.0 40.000004 1 GTATGCC 160 0.0 39.375 42 ATCTCGT 160 0.0 39.375 37 ATCTCCG 160 0.0 39.375 10 TATACAC 160 0.0 39.375 3 TGCCGTC 160 0.0 39.375 45 TATGCCG 160 0.0 39.375 43 GAGCCCA 160 0.0 39.375 16 CACGAGA 160 0.0 39.375 21 TTATACA 160 0.0 39.375 2 TATCTCG 160 0.0 39.375 36 ATGCCGT 160 0.0 39.375 44 CGAGCCC 160 0.0 39.375 15 CCGAGCC 160 0.0 39.375 14 TCTCCGA 160 0.0 39.375 11 TCCGAGC 160 0.0 39.375 13 ATATCTC 160 0.0 39.375 35 CTCCGAG 160 0.0 39.375 12 AGAATAT 155 0.0 39.193546 32 >>END_MODULE