FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l01n01 sv79.3410000000470d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l01n01 sv79.3410000000470d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42740930
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC974810.22807412005307323TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT118350.039.04840533
CTCGTAT121650.038.1943342
TCTCGTA121950.038.0254641
ACGCCAA122550.037.7301932
GTCACGC122800.037.65293529
TCGTATG123850.037.6442343
TATCTCG124150.037.35110539
CGTATGC125100.037.17841744
GATCGGA133050.036.643351
CGTCTGA132200.036.1497116
ATCGGAA136350.035.85742
ACACGTC135500.035.3191713
TCACGCC131050.035.31690630
ATCTCGT134200.034.70475440
ACGTCTG138450.034.5178215
AGTCACG135900.034.30554228
CACGCCA135850.033.93654631
TCGGAAG145600.033.6254543
CGGAAGA152300.032.2049144
CACGTCT150150.031.84314314
Produced by FastQC (version 0.11.2)