FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l01n01 sv78.341000000046ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l01n01 sv78.341000000046ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46296438
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2046340.44200808710164696TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA252450.041.860211
CTCGTAT248300.041.1316342
TCTCGTA248450.041.08801741
GATCTCG247850.040.94144439
ATCGGAA259150.040.9014632
CGTATGC250950.040.8510344
TCGTATG251500.040.83313443
CGTCTGA255600.040.7219216
ACACGTC259850.040.16850313
ACGTCTG263350.039.57480215
TCGGAAG269950.039.273143
ATCTCGT264600.038.66430340
CGGAAGA275400.038.4298444
CACGTCT275350.037.89916614
GCACACG285250.036.81316811
CACACGT291550.036.00930412
GTATGCC289350.035.5858645
AGTGATC324050.031.66099736
GTGATCT335350.030.52705237
GTCACAC337500.030.34680229
Produced by FastQC (version 0.11.2)