FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l01n01 sv77.341000000046e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l01n01 sv77.341000000046e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50501900
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4266270.8447741570119145TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA484000.043.445471
ATCGGAA491400.042.8291362
TCTCGTA481050.042.10896341
CTCGTAT481300.042.09264442
CGTCTGA492750.041.99092516
ACACGTC494750.041.95769513
TCGTATG485150.041.9543943
TCGGAAG504150.041.710073
ACGTCTG498750.041.55795315
CGTATGC488800.041.55368844
AATCTCG487650.041.46938739
CGGAAGA511050.041.0406044
ATCTCGT503800.040.29187440
CACGTCT515850.040.2545714
GCACACG524250.039.7602611
CACACGT529800.039.30069412
GTATGCC528200.038.53588545
ACTGACC566150.035.92330632
GACCAAT571300.035.62570635
ACCAATC575450.035.2046336
Produced by FastQC (version 0.11.2)