FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l01n01 sv76.341000000046c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l01n01 sv76.341000000046c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40002020
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4440941.1101789359637337TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA501250.043.6656951
ATCGGAA505600.043.3978352
ACCGATG494700.042.8991632
CACCGAT494800.042.86742831
CGATGTA495700.042.8381934
CTCGTAT494900.042.80447842
TCTCGTA495200.042.7596841
ACACGTC508450.042.6327313
TCGTATG499700.042.6013443
CGTCTGA507950.042.5992816
CCGATGT498650.042.57106433
CGTATGC498050.042.5573544
GTCACCG500850.042.4484529
TATCTCG499150.042.4117639
ACGTCTG511000.042.3978615
TCGGAAG519450.042.1884353
CGGAAGA521000.041.9409034
TCACCGA507000.041.87584730
ATCTCGT510750.041.48805640
CACGTCT524900.041.28378314