FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_93.351000000436d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_93.351000000436d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125128
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC58704.691196215075762No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT12601.0069688638833834No Hit
GATAGGACAGTAATAAGATATGACCTTTCTGAGTTTCTAGTTAACATGTTT10460.8359439933508088No Hit
ATACAAACACAGGAAGTACCAATACGGTATCATTGGATTTGAATAGAGCAG8830.7056773863563711No Hit
GAATTGGTAAATGAGATTTCTGAAAGTTCTTTTGAATCAAGTATCTCTTTT8450.675308484112269No Hit
TGTTTGGTATTAGCATAACTATGTAGTAAAGAAACAGCTACCGATTGTACT8410.6721117575602583No Hit
TGTATGCAAGATGGGAAAAAGTAAGAGATTGAAAAACAAACCACAACCAAT7650.6113739530720542No Hit
TAACAGTACTTTGTAAAAGGACATGAGAGAAATTGGTAGGTGTTCTACATA7490.5985870468640113No Hit
GTATAAAATTCACTGGTACAGCAGATACACAAAAATGAGAAAGAAAATGGA7160.5722140528099227No Hit
AGGAACAATAAGATGGACAAGGTTACTTTTTCAACGTTTGATGCAAGTAAA7080.5658205997059012No Hit
TCCTAAACCAGATTACGAAATTTCAAAAGAAGATTGGTATGTGAGGAACAA6810.5442426954798286No Hit
GATTTTAATGAAAGCCATATCTGATGTTTCAAATGGACAGAAAATATCCGA6450.5154721565117321No Hit
AACAATAAGCGTAAACATTTGCCATAGTTCTTTATCACTTTTTACAAGTTG6180.4938942522856595No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT6030.4819065277156192No Hit
ATAATGAACCTATTGCATTCTTTATTATGATTCCGGAGTTGAATCAGATTT5570.4451441723674957No Hit
CAATACAATAAAAAACTGTGTGCATTTTATTTTGAACGTAAAGAAGCTTTG5120.4091809986573748No Hit
CCTTAGATTTAAAGTTTCTAAAAGAAAGATACCAGGACACATCTTTACAAG4620.3692219167572406No Hit
ATATATACAGTGAAGAACTGAATAATGCCAAGCAGGAATATATACTGACAC4590.3668243718432325No Hit
ATTTAATACAGTTTTCTCAGTGACTGATAGAACAAACAGAAAAGGAATCAG4520.3612301003772137No Hit
GTATAAAATTGTTGTTCTGAAATCTCTTCAACTTTCATTTACAATGCATCC4430.35403746563518956No Hit
GATGAATCCATATTCTCATCACTAAGATCATCCCATATGGGTTTTATTGAA4240.33885301451313854No Hit
ATACTAGAACAAAGATGATGATGAAACTGACAATGATGATTATGTCACAAA4220.33725465123713316No Hit
TTCACAAACAGTGATAAATTCTTTACCCGCTTCGATTTCAACCTTGATAAA4170.3332587430471197No Hit
ACCATCTATATTGTCAAGTTTGTGGTCAAGCTTATCCTACATATGAACTCA4130.33006201649510897No Hit
TCTCAAGATAAGAAACCATTGTCAGAATTTGTAACTAAAGAAACTGCAAAC4110.32846365321910365No Hit
CATACATGTATGTATTCCACCAAAAACCTATCAGTGATTAATGGATTGTGA4000.3196726552010741No Hit
ACCTAATTGTTGATCATACAATGTTGGGAACTGGATATTACGTGAAGTTAG3920.3132792020970526No Hit
ACTCTATTCTCATAACAGAAGGTAGATTGCAAGGTTTATCATTTCGCCATT3880.31008247554504187No Hit
GAATTCAAAAGAAACATCTGAGTTTTTAGAAAAGTAGCAGTTATGTAATCA3840.30688574899303117No Hit
ATCCATATAAACGCATGCTTATTGTTTATCGAGGTATAAACTAAAGGTATC3720.2972955693369989No Hit
ATATTCTTGCATATCATCCAGTGAAGGATTTAGCTCTTGTTATTTTACAGC3640.2909021162329774No Hit
CGTTAACTGTTAGTGATTACAAGAGAAGGAGAGGTCTTCTCTGATTTATTT3560.28450866312895595No Hit
ATATTGAAACATGGGAAGAGTTTTGTAAAGCAACAGAAAGATTATATCAAA3490.27891439166293713No Hit
TTACTGCATACAGTAGATACGTTGAATAAGAACAATGATATCGATGGTATC3250.2597340323508727No Hit
CAATATAATATTGATGTCAGAGCTAAAAGATGCATCAAAAGGGTATATGGT3210.25653730579886197No Hit
ATACTTTATTGTGATTTTTGAGTTGCATATTCCTGATGATTAGTGATACTG3210.25653730579886197No Hit
GATATACCAGAGTTATCTAGAAAGCTTTTAAAAGTATACTCAGACCACTCT3200.2557381241608593No Hit
AGTTTTTATGATCCACTTAGCTTAATAAGCTCATGTTTCTTCTCATTTACA3080.24614794450482708No Hit
GTAACATTTTTTGTTGTCTCTTTTTAATTTGTCTCGAGTATAAACGACATT3030.24215203631481363No Hit
GTATATTCCTAGATATCTTAGGGTTTTGGGTGCAATTGTAAATGGGACTGA3010.24055367303880826No Hit
ATTCATATTCCTTTCTGTACAATGGCAGTGCATTGAATTCAATGCTCAGTC3000.23975449140080557No Hit
GTGCAGAAATGAAATTTTACCCTGAAATTTGAAATTGTTCATTCTATGGTG2990.23895530976280288No Hit
GTACGGGATTCTATTTGAATAAGCAATGTATTCTTCAGATCTTCGTCATTT2990.23895530976280288No Hit
GTACATCACTACTAACTTCTAACGTATACCGCTAACTTTTTTTACTTTCTG2980.23815612812480022No Hit
GTTATATACACATCGATTTTTTTAGTTTTCCACAGAATGTACGCTTTCCTT2970.2373569464867975No Hit
TAATGGCTGAATAATATTTCACACAGTTCTATAGTTGTTGCTTCCATTTCA2950.23575858321079213No Hit
TATTTGAATTACTTGACTTACCAAACCAACAATTGTACATGGTTAAACTAA2900.2317626750207787No Hit
GCATTTATGACAAAGATTTTGATGATGTTCAAACACATATTGAAGAGAGCA2810.22457004027875457No Hit
CTTTTAAAGTACATCAAAGATAAATACGCAAACCCGGAAATTATGATCATG2740.21897576881273578No Hit
ATTATGTGGAAGGAGAAAGAAATTTTCTTCTCTGGTCTTGTATGAATGCGA2680.21418067898471962No Hit
GTCTTTTCCTTTGTTTTTGTTACTTGACACAGTTTTTTTTTTTGTTTCCGG2670.21338149734671696No Hit
ATATTGGGAAGACGTTTTATTCGTTTTGGAAAACCAGACTCGCAAATGTTT2670.21338149734671696No Hit
ATATCTTGCAATCACTTCAATCAGGTTCATATCTTGATACTCAAGTTGTAT2650.2117831340707116No Hit
ACAGTAATTGCATTAAAGTAGCTGAATGCTTTTCTATCTACATTTTGACTG2650.2117831340707116No Hit
GTCTAAAAGTAACACTGATTTAATGCAGTTTGCATAATTTACTAATCTTTT2640.21098395243270893No Hit
AAAAAAGACAGATAACTCAGAATGATATTTGGCGAAACTTTCACCTTTTGA2640.21098395243270893No Hit
ATATTATGTTCTGCTGTAGGGTTGTCTCCAATTTTTTTTTGCTTTACGTAC2640.21098395243270893No Hit
GTTTTATTGTTGTGGATAGATTTTTAGTTTGCTTTAATACCAAGAAGGATG2620.20938558915670355No Hit
ATGCTTTCACCATTAATTACACGATTGTATGCCTCTTTACCTAGTCCACCT2600.20778722588069817No Hit
AATAAATCCTACATTGTAGTAACTTAATCCAAACCTTTGTAAAAACTTTGT2460.19659868294866056No Hit
GGATTAAGAATGCTTCAATCTCCACAAGATAAAAGACACATATCAGAAACG2430.1942011380346525No Hit
TTTTATATGTGCACTCTTCAGAACAATAATGGCTGCCTCCTTTTCTTTTTT2430.1942011380346525No Hit
CCTTAAGGCTTACCTATCAGAATGTGAGGTATTTATTTGTTGAAAGATGTG2410.19260277475864715No Hit
AGAAAGAACCTACTTAAACTACTTAGGAACCTACTTTACTTAGTACTAAGG2390.19100441148264177No Hit
GAATCAAATTGAAAAGAATCCAATCTAATCGAAAACATCGAATTGAATCGA2380.19020522984463908No Hit
ATATACAAACATGCATCTCTCTCTATTCTATACGACGAGTTCATCTTCAAG2370.1894060482066364No Hit
CTTCAGTAACAGTTAATTTTCCCCTTTTCTCATTTTATTTAGCCACGGAAT2370.1894060482066364No Hit
TTTCAGTATTTGTCTGCAGTTATTTTTATGCGTTCAAATAAGTGTACAGTC2350.18780768493063105No Hit
ATACTGATGATCCTGACCTTATTGATTCTAAAAGAGTTATTACCCCAGATG2330.18620932165462567No Hit
ATTGGTAGTAATTGCTTTTCATCCATTTTCTCAAAGAATTGGAAATAGGAT2270.18141423182660954No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.17821750527459881No Hit
ATGTAAAAGGAGAAAAAAACAGATCTTAGGAAGAAATGACTCTACAAGAAA2200.17581996036059075No Hit
TTGTTAAGCCGTTACTGTTCCATCTTCTGTAAATCTATCAGCAATATTGCC2170.1734224154465827No Hit
CAAATACTAGAAAAACAGATGGTTATTAAAGCTTAGAAATTCCCCAAGAAT2130.17022568889457196No Hit
ATATCAATGTTTCATCATATTTGTGTATCTTCCTGCCATAATGCAATGACA2110.16862732561856658No Hit
ACACCAAATGCAATGAATATTTTCCTCTTGGGAAAAGTAACAACAGATATG2100.1678281439805639No Hit
ATATATTCCAAGTGAGCATGGTTACAACCTGCTATAAATATGACATTACGT2090.16702896234256123No Hit
CTCGTATGATTGGTTTGAACAAAAAAACACATACAGAAAGAAGAACTCCTT2090.16702896234256123No Hit
TTTCTATGCTTGTATTTGACTTATCTGGATGATGTTTATGTACTTTGTTTT2050.16383223579055048No Hit
GTTTACCAAACATTTACTGTTTTCAATAGGGTATCAAATTCCAAACAATAA2040.1630330541525478No Hit
TATTGATGGTTTTCTTTGTTGTGCAAAAGTTTTCAATTTTGATGTAGTCTC2040.1630330541525478No Hit
AAATAAAACTGAGTATTTTGCTTTACTCCTAAATTCTCACTACAATTTCTG2030.1622338725145451No Hit
TAATCTCCTACTGGGGATACATCTTCTATGCTGAACAAGGCTTAAATAAGA2020.1614346908765424No Hit
TAACAATTCATTCAAGACTTTCTTAAAAAACATGTTGCCACATGAACTCAG2000.15983632760053704No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.15983632760053704No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2000.15983632760053704No Hit
TGATTAATAAGCTGCTTTTTCAAACAAAATTACTACTTGCTCATATACTGT2000.15983632760053704No Hit
TCTTCTGATTATGTCTTAAACTCAATGTGAGACTCTTTCGTATTAATGGTT2000.15983632760053704No Hit
ATCCAAAACAACGAAATTTTAATTTGAGGTGAGTCTATTTCTCTGCAATTT1960.1566396010485263No Hit
ATATGGTCTATCTTGTTAAGTATCCCATGTTCACAGAAAATGTATATTTTG1930.15424205613451825No Hit
AAAATACTCAAATGGTAGTAAACGTGTTATGTTTGTTGATGCTGTAAAAAA1900.1518445112205102No Hit
TTATTGATCTAAAGTCATCACTATAATGTCCATCATAAACTGTCTCTTATA1890.15104532958250752No Hit
ACAACAAACTTACTTAGGAAACATCATTTTACTTGACATATCCATGCTACC1890.15104532958250752No Hit
AAAGTATACAGCACAACATGTTAAACACCTACTTAATAGCTAGCACAAACT1880.15024614794450483No Hit
ATGTAAAGTCTTTCAAATGAAGTCAATTTTTACGTCACTACTTATATTCCT1880.15024614794450483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1880.15024614794450483No Hit
GTTCTATAGAATGGAATTTTCTTCTTCTCCATTGTGCTACTGAAGAAAGGA1860.14864778466849946No Hit
AAGGAGAAATATCTTCTGTTCAAGTGAATTCTGAAAATGTTTCGAATGTAG1830.1462502397544914No Hit
ATATCAATTAAGCTCATTGGATTCCAGCTAATTAAGTTACAAAGATAAGGG1820.1454510581164887No Hit
GTATATATAGCTTCAGTATCGCAAACAAATTTGTTGTTTCCATCTGATCGA1790.14305351320248066No Hit
GTTATAATAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1780.14225433156447798No Hit
TTCAACATGTACATGACATTCCTCCTAAGACGATTCATCCAAAAAACACAA1760.1406559682884726No Hit
GAGTAGCAATATTCAACAAAATTACTTTGTTGGTGAATAAAGCAAATAGCA1760.1406559682884726No Hit
ATATGGTTCATTTTTATTGAGAATGTAACAGAAACACAGAAGGGAAACTAT1750.1398567866504699No Hit
ACTACTACTCTAGTAGTAGTAGACTACTAGTAGTAGTAGTCTACTACTACT1740.13905760501246722No Hit
TTATTATACGTAAATGCCTGTTTGGGATCCAAGGCTAATGATTTATCAAAA1730.13825842337446453No Hit
GTAATATTCCATTGTATACACAAACCACATCTTCTTTATCCATTCATCTTT1650.13186497027044308No Hit
GTACATAACAAAATGAAGGCAGAAATAAAGATGCTCTTTGAAACCAACAAG1650.13186497027044308No Hit
GCTAGACATAGATCATGTCTTTTGGATAGTATGGATTGAAAACCGAAAATA1630.1302666069944377No Hit
AAGATAAAAGCACGGACTTCCATCTAGTTACTACACTCATTTTTCTGTTTA1630.1302666069944377No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1620.12946742535643502No Hit
ATGTAATCCTCATCAAATGGAATCAAAAATAACCATCATCAATTGGTATTG1610.12866824371843233No Hit
AACTATAAAGGCAATAAAAGATGACAACTGAAAACAACGAAAAACTTTCTT1610.12866824371843233No Hit
ATGATGCCATTATCTTTGAAGCTGAAATGGTTAAGGTCTCTGTGACAAACA1590.12706988044242695No Hit
ATTCATTGGTGATTCTATTCAATTCAATTCTTTGATTCAATTCCATTCCAT1590.12706988044242695No Hit
GTACAACATCAGCAAATCAATCAACATGATACACCACATTAACAACATGAA1580.12627069880442426No Hit
GGTAAAAGTCTTCAGTAATTTTTCTTCCCCAATATTTCTTTCATTAGTCAT1570.12547151716642158No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.12467233552841889No Hit
GAAAGTAGTGTGTATGATGTAGTAGTGCTTCTAAAATTGATTTCGTGCTTA1560.12467233552841889No Hit
ATTTTATACCGCAATATGGGTTGGAAATAAAGCTATTTAGCTGATTTTCAT1560.12467233552841889No Hit
AAAATATGGTGTGAATATATCAGTGATTGCTAAAGGTTGGGGAGTTTGTGA1550.1238731538904162No Hit
GTAGTACTACCTACTAGTAGGTAGTACTACCTAGTAGTAGGTAGTACTACT1550.1238731538904162No Hit
AGGTATCAGTTTGTAGGTAAATATTTAAAGTCACCACATTTGGCAGAGAAC1540.12307397225241354No Hit
GAATAAGATTGTTGCCGCTACTTAAATCTTTAGTTTTGACTCATCTAAACG1540.12307397225241354No Hit
CTTTTAGGTTTTCTAGTTAATGTGCATAAAGGTGTTGAGAGTAGGTTTGAA1530.12227479061441085No Hit
AAATAAGACATCCAGATGGCTAACAGACACATGAAAAAATGCTCAATACCA1530.12227479061441085No Hit
TTGTAAAGTTGAAAAATCAGAACCTCAATGGGATGATGTAACTTCCCCAAG1530.12227479061441085No Hit
TTATATGGCTATGACATCACTAGATTATGGGAATTTTTCAGCTCCATTATG1520.12147560897640816No Hit
ATGTACTTTAGAAAAGGATAAAGGGTAGGTAAAAGATATCTAAGTCTCTCT1520.12147560897640816No Hit
ACCTATTCTTCTCAAACTATTCCAAAAAAAATCGAAGAGGAAAGAATGGTT1510.12067642733840547No Hit
CATCATTATGGTATAGCAGATAAATGTTGATACAACTTTACAAGGAATTCT1510.12067642733840547No Hit
AAATATGGGTCATGTTTTTGAATGAATGAGCAAAATGTTGAGTTTAGCTGA1500.11987724570040278No Hit
GTATAATATGCTTTTCAAAAACACATTTGTTCCTTCTTATGCTAAAGGATG1500.11987724570040278No Hit
TAGTAGTGATCTACAATCATATCAATAAGCGCATAGCATAAATACTGTCTC1460.11668051914839205No Hit
TTTTTACTTTGGTTATTTTGTAGTTCTTCACTACTTTTTTCCATTTCCTGA1440.11508215587238667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.114282974234384No Hit
GCTTTATTCCTAGTAGATATTGGTCATGAGCATATTGCTTTAGCAGAAGCA1430.114282974234384No Hit
ACCATCATCAGTAAAACTTCATTCTTCCTGTTTCACAAGATGTTGTTCTCG1430.114282974234384No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1420.1134837925963813No Hit
GTATTAGTTAGGGGAATATGGAGAATGGTAATTGTATTGACTTATGACTGT1420.1134837925963813No Hit
TTTCTAGTTTTTATCTGAGGATATTCAGTTTTTCCCCTTAGGTTTCAATGG1410.11268461095837862No Hit
GTTTAAACGTTATAGTATGTTCTTGCAGTGTATTGGCATTCAAATGCATTT1370.10948788440636789No Hit
ATCTTCATATGTTTTGAAAGTAATAGTGTAACAGGCCCAAAAGTCTTTATC1370.10948788440636789No Hit
ACCTGAATCTAGTCATGAGGAAAAATCAGAAAACCAAAATTGAGGGACATT1360.10868870276836519No Hit
ATATCTTCAAGTTCAATCATTTTCTGATCTTCATCCATTGTTACCTTACTG1350.1078895211303625No Hit
ATTTAGACGAGTTTTGGAAAATGTTTTCGGTTAAAATGTGTCAAAATATTG1350.1078895211303625No Hit
GATTATAACAGGAAATACTATCTTCAGAGGCATCTTCTAGCTATGAACGAA1340.10709033949235981No Hit
CAGGAAGATCATATTCAAATTCTGGATTGTGATGATGGTACAAAAAAGAAC1340.10709033949235981No Hit
CTGTTGATTCACATGACATCTTGAAAGAATATGAAAGGCATTTTGCTGAAT1330.10629115785435715No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1330.10629115785435715No Hit
CTATATTCTCCTCTATTTTGATAGTTTCCTATCTCACATTTAGGCCTTTCA1320.10549197621635446No Hit
ATATATAACAGCCCTCTGATTGAAAAAGAACTCTTCCTGTTGCAGCTCTTT1320.10549197621635446No Hit
GTATAAGAAAGTAGTCTGATTGCATTCCATCCTTTTGCAGGTAGCTATCTA1320.10549197621635446No Hit
GTCACTATCTTATCTGGAAGACTGAATCATTTTAAGAATTGGATACTGTTA1310.10469279457835177No Hit
TATTAATACTTGTTGTGCTAGATTGGTTTTAAAAGTTCGGAAATTAAACTT1310.10469279457835177No Hit
TTTTATTATGTGCCAAGTCCTAATGGAAGGATTGGGGATACAATGTAGAAG1310.10469279457835177No Hit
GTTATTTATAAATGCTTACATGTGTGGCAAAAATAGTTGATATCCAGAAGA1300.10389361294034909No Hit
GTTCACATTTATTTACAAACGGCGAACAAGAACATTAAAATCGGAAAAGAA1290.1030944313023464No Hit
TCTTTACCCTGATACATATTTTTTATTGCTTCAGCAGTTACAAGTCTTTTG1290.1030944313023464No Hit
ATTTCTAAGGACATAAAAATGACGCTTCAAAGAACACTGGACTCTGGATAT1280.1022952496643437No Hit
TATCAGTACCGTAAAAAATGTATTAGACCAGTTGAATTAGTAGATAGTGCT1280.1022952496643437No Hit
AGAAAATCCTTTACTACATTAGCAAAAACGAAAAAAACCAAAACCCAAACC1280.1022952496643437No Hit
GTTAGAGAGCTATAGTTTGTAAGGCCTCATATTAAATTAATCAGGGATCTT1270.10149606802634104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC800.045.0155943
CGTATGA253.862917E-545.0155943
TTTGTCG206.997217E-445.0155942
TGAACCT600.045.0155945
CCTAATT406.710252E-945.0155942
TTATACG253.862917E-545.0155944
CGTTAAC502.1827873E-1145.0155941
GTCAGAT253.862917E-545.0155943
GTTAACT502.1827873E-1145.0155942
TTAAGGC206.997217E-445.0155943
TCGTCAG253.862917E-545.0155941
ATAAGCG600.045.0155945
AACCTGA206.997217E-445.0155946
AGTTAGG253.862917E-545.0155946
TAAGGCT206.997217E-445.0155944
CTGATTA206.997217E-445.0155945
CGTCAGA253.862917E-545.0155942
TGGTCTA206.997217E-445.0155944
GTCGGTT206.997217E-445.0155945
CGCCATA206.997217E-445.0155941
Produced by FastQC (version 0.11.2)