H23LNBCXX_l02n01_bir_1228_pcontrolb_9.35100000042c5a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_9.35100000042c5a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26632
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	4050	15.207269450285372	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	332	1.2466206067888255	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169	0.6345749474316611	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.5444577951336738	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	139	0.5219285070591769	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	134	0.5031541003304296	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	128	0.48062481225593273	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	128	0.48062481225593273	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.46185040552718537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.44307599879843795	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	116	0.435566236106939	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112	0.4205467107239411	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.3454490838089516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.3416942024632022	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	91	0.3416942024632022	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78	0.29288074496845895	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78	0.29288074496845895	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	75	0.28161610093121053	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	70	0.2628416942024632	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.2628416942024632	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	66	0.24782216881946534	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	53	0.19900871132472212	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	47	0.1764794232502253	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	44	0.16521477921297686	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA	44	0.16521477921297686	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT	41	0.15395013517572845	TruSeq Adapter, Index 12 (95% over 22bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	41	0.15395013517572845	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	41	0.15395013517572845	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.14644037248422948	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	38	0.14268549113848003	No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	31	0.1164013217182337	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA	30	0.11264644037248424	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	29	0.10889155902673475	No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	29	0.10889155902673475	No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.10513667768098528	No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.10513667768098528	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	28	0.10513667768098528	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.10138179633523581	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	415	43.915665	45
ATGCCGT	430	42.38372	44
GTATGCC	455	40.054943	42
TGCAATC	455	40.054943	33
ACTTGCA	455	40.054943	30
TATGCCG	455	40.054943	43
CAATCTC	455	40.054943	35
GCAATCT	455	40.054943	34
TCTCGTA	455	40.054943	38
CGTATGC	455	40.054943	41
CTTGCAA	455	40.054943	31
TCGTATG	455	40.054943	40
CTACTTG	455	40.054943	28
CTCGTAT	455	40.054943	39
CCACGAG	450	40.0	20
CACGAGA	450	40.0	21
ACGAGAC	450	40.0	22
CGAGACT	450	40.0	23
AGACTAC	450	40.0	25
GAGACTA	450	40.0	24

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content