FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_85.351000000435d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_85.351000000435d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98683
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC3594636.42572682224902No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT1547015.676458964563297No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG71857.2808893122422305No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5520.5593668615668351No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3240.3283240274414033No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTAT2790.28272346807454174No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2730.2766433934922935No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2420.24522967481734442No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA1780.18037554594003022No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCC1660.16821539677553377No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGC1580.1601086306658695No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1480.14997517302878915No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1270.12869491199092042RNA PCR Primer, Index 44 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37500.043.9245
GCCGTCT900.040.045
ATGCCGT52950.031.06232544
TATGCCG61000.026.96311443
GTATGCC61400.026.75081442
CGTATGC61750.026.70850241
TCGTATG61750.026.63562840
CTCGTAT62000.026.52822739
TCTCGTA62200.026.51527438
ATCTCGT62350.026.4875737
TATCTCG62350.026.41539836
ATATCTC62500.026.38799935
AATATCT62500.026.31634
TAATATC62800.026.26194233
ATAATAT62700.026.23205832
CATAATA62850.026.1336531
ACGAGAC63450.026.09929322
CACGAGA63650.026.0879821
CGAGACA63400.026.08438523
CCACGAG63750.026.04705820
Produced by FastQC (version 0.11.2)