H23LNBCXX_l02n01_bir_1228_pcontrolb_84.351000000435c7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_84.351000000435c7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101130
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	11258	11.132206071393256	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	1287	1.2726194007712845	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG	736	0.7277761297340057	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	296	0.29269257391476317	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	244	0.2412736082270345	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.17798872738059923	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	174	0.17205576980124593	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	166	0.16414515969544152	No Hit
TATTAATGCTGGGTATCATGATGCTTTTTTTATCAGCAGCTGAAAAACTAA	160	0.15821220211608822	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	148	0.14634628695738158	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143	0.14140215564125383	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	141	0.13942450311480273	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.13546919806190053	No Hit
CTTTAGAACTGTGAAAGTAGTAAGAGTATTAGGCTGACTTCTATAATCACT	129	0.1275585879560961	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127	0.12558093542964502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.1236032829031939	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121	0.11964797785029169	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.11668149906061505	No Hit
CAATAGAGAAATTACTGCAATACCTAATTTTATTGACGTTTCGCGCTTTAG	115	0.1137150202709384	No Hit
TTCTTAGACACTTATATTTGGGTTGTCTCTTATTTAGAACATGGATGCTGA	112	0.11074854148126174	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110	0.10877088895481063	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	110	0.10877088895481063	No Hit
AACTGGTATTGAACTGATTACTCCAACGATTATTGAATCTTGTTTTTAACT	108	0.10679323642835954	No Hit
TGAATATTGAAACTGAATCTCGTTCGTACTAATATTTGAACCACCCTGAAC	107	0.10580441016513399	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAA	20	7.0027664E-4	45.000004	11
ACTTGTG	20	7.0027664E-4	45.000004	27
CTGAGTG	20	7.0027664E-4	45.000004	45
TTATGAC	20	7.0027664E-4	45.000004	3
GAGTAAC	20	7.0027664E-4	45.000004	1
GTGATCA	20	7.0027664E-4	45.000004	25
CGATTAT	20	7.0027664E-4	45.000004	27
GAGAGCT	20	7.0027664E-4	45.000004	38
TCCTATA	20	7.0027664E-4	45.000004	37
AAGTTCC	20	7.0027664E-4	45.000004	7
AATTGAG	20	7.0027664E-4	45.000004	26
CTGCCAT	20	7.0027664E-4	45.000004	18
CAGGCGT	20	7.0027664E-4	45.000004	11
TATTAGA	20	7.0027664E-4	45.000004	2
ATGGTAC	20	7.0027664E-4	45.000004	37
CCAACGA	20	7.0027664E-4	45.000004	23
CCTATAG	20	7.0027664E-4	45.000004	38
TACATCC	25	3.8655307E-5	45.0	4
TTGACGT	25	3.8655307E-5	45.0	33
GGCTGAC	35	1.1972952E-7	45.0	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content