FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_83.351000000435ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_83.351000000435ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151173
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC131228.680121450258975No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT22081.4605782778670795No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG9650.6383415027815814No Hit
CATGTATATGTGAACATTGATCTCTTAAAGTTTCCTTTCTTGTGTGTTCAT3130.20704755478822273No Hit
ATAGTCTTTTCAATGTCTTCCATAGATGAGTTTTCGTTAGCATTTACTTTT2910.19249469151237325No Hit
TTTGTGTTCTTACTTTGTTTATCCATTCAAGATGAGTTATGATTGAGGATG2820.18654124744498024No Hit
ATGAAGATATACCAGGGAAATGCTAACTAAAAGAAAGCAGAAATGTCAATA2460.1627274711754083No Hit
TACATACTCTTTCTTTAATTGCTCTGTTTCTTCTTCTCCAGGATCCATACT2420.16008149603434477No Hit
AAGTAAAGTGTCGATTAATGACATGATGTTAGCAATGTATTCTGTCTCTTA2340.15478954575221765No Hit
GATAATAGTTAGGTTCCCTGATCTAAGAGAAACAATTTGAGTGTTGAGGAT2330.15412805196695176No Hit
GATGAGAATTGATTTCATTGCTTTTCTCACACTTAGTTAAGAAGGCAATGA2220.14685162032902702No Hit
TACTTATACGCAATGAAGTACTATTCAACCACGAGATTCTTTTGCAACAAG2140.1415596700468999No Hit
ATTTTGTATAACTGACCTTAGTGAGATCAAACTTACTTCATGGGATATAAG2120.14023668247636814No Hit
GAAATATTCAAAGTCGAAGATTTACATTCCTTTCGTATCACAATTAACCTT2110.13957518869110225No Hit
TAATATCGGATGATGAATAAAATCCATATGTGCACGAATTTGATGTGTTCT2100.13891369490583635No Hit
GTATTCACTCTGCTATTATCAATTGTTAGGAATGGGTTATAATTCAGGGAT2090.13825220112057046No Hit
AAAGAAAGGAAAAAAAGAAAAAACAAAACAACAGCAATAAAACAACAAAAA2060.1362677197647728No Hit
GTTATCTACATAGCGTCTGATATTGAGGGTATATTCATTTTCAGCTATGGT2020.13362174462370927No Hit
ATGATAATGGTGGTGATGATGGTGGTGATATGATGATAGTGATGATGAGAA2000.13229875705317748No Hit
TATTTACAAGGAGGCTGACTGTAGCATACTCTTAACTATCCATTTAGTCCA1960.12965278191211393No Hit
CATAAAGTGAGTCAATGCAGATATAAGAAGCATTTGTAATGTGAGAAGCAA1950.12899128812684804No Hit
CTACAACAACAACTGATATGAGATTGGATGTACACAAACAATGTATAAGGA1950.12899128812684804No Hit
GAATGACTACTGGGTACATAAAGAAATGAAGGCAGAAATAAAGACATTCTT1930.12766830055631628No Hit
TAGTAGTACTACACCGATTCAAAAGTATTTCAAATCGAAACACAAAACATC1910.1263453129857845No Hit
GTTTTGTTTCTGAAATTCCACATATGAGTGAGATCATATAATTGTCTTTCT1890.12502232541525274No Hit
TATTTGTTCTGTGTATAATCTCAAAACAAGGGAAGGAAATGTGAGAGTTTC1870.12369933784472095No Hit
ATGGAATGGTATGGAATGGAATGGAATGGTACGGAATAGAATGGAAAGTAA1850.12237635027418918No Hit
GTAACAACACTAAGTGCCATATACATGCATCTTGGCAATGCTTATCAATAT1830.1210533627036574No Hit
TTTTCATATCATGCCAGTAAATCTTACCCATTAACTGTCTAGCATATTTTG1830.1210533627036574No Hit
AAGCAATGGTTATGTTACGTACTAAATTATACGAAGAGCAAGTACGTAAAC1820.1203918689183915No Hit
CTACTACTTACTCATTTCTACTTAACATTCTAACGATGGCAAACCAAATCT1800.11906888134785973No Hit
GATCATGATAATAGTACCCAGATCATAGGGTTATGAAGGTTAAATTAGCTA1790.11840738756259384No Hit
GTTAATGATCATAACATCTCACTCTCAGAAGAGTTACTTACAATTTTGGTT1770.11708439999206206No Hit
AATTTGATTACACTTTACAGTTACGAAGTCTTGAAACTTTGATTCGCAAAT1760.11642290620679618No Hit
ATCTTAATGTTCTTTGACAGGTACATCCAGAGAATGGAATACTATTCCTGT1720.11377693106573263No Hit
GAATCATCAAATGGAATTGAATAGAATCATCGAATGAAATCGAATGGAATA1690.11179244970993497No Hit
ATCCTAATAGGAGAATAATGGTATCTCATTGTGGCTTTAATCTACATTATC1690.11179244970993497No Hit
GAATAAATTTTGTGTCTGTTTCATAGTTGCTACTACCATCTTTACTGGTTA1680.11113095592466908No Hit
ACATAGTACTGTGAAGAAAAAAAGCAAAACAGGGAAAGTACTGCTATTAAG1670.1104694621394032No Hit
CAATATACATTTCTCTTCAAGTTCTTGTAACACAAATGTAGCTTTGTTCAA1660.10980796835413731No Hit
GTAGATGAACAGATAAAGAGTTTAGGATTAGTTATGGCATGAATAAGTCCT1660.10980796835413731No Hit
GTACTGTTACTGTTGTTTGGTCTTAGACTGATTCTTGTTGAAATTACGAAC1650.10914647456887142No Hit
GAATGAACTGGAAGAAAACGAACAAACAATAGGAATTTTCTCTCCTTTGCT1650.10914647456887142No Hit
GATAAGTACAGTGAAGTTCGTTTTTTGTTTTGTTTTGTTTTGTTTATGTTC1640.10848498078360554No Hit
ACTTTAAGTACTTTGTGTCTTGTTATGTTTTTGAGTCTATGACTCCTGTCT1630.10782348699833964No Hit
GAATTATTCTTAGGATGTTCTTCCAATCATACACATTGCAATGTCTGTCTC1630.10782348699833964No Hit
ATTATCATATCATCAGCAGCATTATCACCATTACCATAATCATCACCATCA1600.10583900564254199No Hit
ATTTTTAAGGCTGAATAATATCCCATTGTACGTACATAACACCTGTCTCTT1590.1051775118572761No Hit
CTGTTACCATTTTGGTAGTATATCAAAATGCACTAGTTCATCATTTCAGTA1580.10451601807201022No Hit
TCATAAGGAGGACAGTAATCAACGTATATGAGTTTGGGACTTAGAAATCAA1570.10385452428674433No Hit
CTATTAAAAGACAAAAATCCAACTGAGTAAACTTACAGATCAAATTGGTGT1570.10385452428674433No Hit
CATATGAGTAAGAACATGCAACAAGAATAAGAACATATGAGAAAGGCAAAC1560.10319303050147843No Hit
TATCACTGTTTATCCTGTTTTGTTTCAGCAAGTACTTATGAATCTCATTAG1550.10253153671621255No Hit
TATTCATACAAAGAAACATTAGACAGCGATAAAAAGGAACAAACTCCAAAA1550.10253153671621255No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGT253.8614104E-545.0261544
GAGCTCT206.9937785E-445.026155
ATGTTCG206.9937785E-445.026155
AAGCAGC207.005198E-445.011256
GACAGGT253.876543E-544.99636516
GGTGTCC253.876543E-544.9963658
GCGAACG253.876543E-544.99636532
TAGCGAA253.876543E-544.99636530
TCTTAGG253.876543E-544.9963658
GTGGACC253.876543E-544.9963657
CCACTCA253.876543E-544.99636510
GCCTGGA253.876543E-544.99636533
GAGGTCC253.876543E-544.99636541
GTGTCCT253.876543E-544.9963659
TCAGACT253.876543E-544.99636535
ACGCTTA253.876543E-544.99636536
CCCCATA253.876543E-544.99636521
CGTGTTA253.876543E-544.99636529
CCTGGAG253.876543E-544.99636534
GGACCTA253.876543E-544.9963659
Produced by FastQC (version 0.11.2)