FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_82.35100000043584.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_82.35100000043584.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89843
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC1903621.188072526518482No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT83399.281747047627528No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG27513.0620081698073305No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6160.6856405062163997No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2530.2816023507674499No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT2180.24264550382333627RNA PCR Primer, Index 44 (95% over 22bp)
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG1590.17697539040325902No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1250.13913159622897722No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCC1200.13356633237981813No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1030.11464443529267722No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA930.10351390759435904No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT910.10128780205469541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAT206.979792E-445.0245131
TTTGACA206.998989E-444.99944318
TAGTGAC206.998989E-444.99944332
TGACCTG206.998989E-444.99944335
GATACGG206.998989E-444.99944320
TCTGGAC206.998989E-444.99944335
TAACGAG206.998989E-444.99944334
ATACGGC206.998989E-444.99944321
CTTATTA206.998989E-444.99944327
CTGGACT206.998989E-444.99944336
GGCTTAT206.998989E-444.99944325
GTCCACA206.998989E-444.99944312
AGTGACC206.998989E-444.99944333
CGAGCAG206.998989E-444.99944337
CGGCTTA206.998989E-444.99944324
ACGGCTT206.998989E-444.99944323
TTAACGA206.998989E-444.99944333
ATATTAA206.998989E-444.99944326
CAGTGCA206.998989E-444.99944341
GACTTAG206.998989E-444.99944339
Produced by FastQC (version 0.11.2)