FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_79.35100000043527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_79.35100000043527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67562
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC39275.812438944969065No Hit
ATTAGAAACTGAACGAAAAGTAGAACGTCAGTTATTCCAAGTTCATTGTGA6380.9443178117876914No Hit
ATTTAGATGATCCTTCTAAAAAGAAAAGCAAAAATGAAATTCCTGAAACGA4340.642372931529558No Hit
TATCTGTACTATAGAACTGAGATTTGAATGCAAGAATGCACTCCATTTTTT4030.5964891507060182No Hit
GTTGTATGCATGTACAATAAAGTGAACAGAGATATAAGCGCAATAACCTGT4000.5920487848198691No Hit
GATGTAGACAGCATAGCTTACAATAGTTTCGATGAAATAGATAGTACGTAT3970.5876084189337202No Hit
CTATAGCTATGATCTAAGCCGATATACTTATCACTAACTATCCTTCCATCT3530.5224830526035346No Hit
AGATAAACGAGTATCGTAAAATAAATACCTGCCTAACAATTGTAAATCCTC3450.5106420769071371No Hit
GTATTGATCAATACTGGAAATATGAGCAAGTATCATGATTTTGTTTTTTCA3220.4765992717799946No Hit
GTCATTAACAATTTGAATGCTTAGATACTCTTCGGTCAGTTCAACAGGAAT2950.43663597880465355No Hit
ATGTAGCCTACTCAAACATGTAACTGATAAGGAGTCTTATGTTTCTATGAT2860.4233148811462064No Hit
GTACAGGTGTTATATCAAAAGATCCCTCTGTTAAAACAAGCAAAGATGATT2820.4173943932980077No Hit
AGACTGTATATACTGAATAGCTTTAGCAGGGTTATGAAAAAGTTGATCATT2740.4055534176016104No Hit
CTATTTATTGGAATGTCAATTTAGCTTTGAGTACGAAATCTATCATCAAAT2590.3833515881708653No Hit
ATTACAACCTATTTGGGCGATTATTATGTTGCACTTTCTTCTGTGTTTAAC2570.3803913442467659No Hit
TGTAAATACTCACAAATTGAACAAGCGTTTTTGCAGAGTTGATTCTTTTAT2560.37891122228471624No Hit
TGATTCATCTTTTAATCATAACTTTCCTGGGCATATTTTTTTGCCTGCAAT2530.3744708563985672No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT2390.3537491489298718No Hit
ACTTTTCTACGATTATCGAATACAAATCGTTAGGATTAAACGGTTTTGTAA2370.3507889050057725No Hit
GATCTACATAGAATGCTAAAGATGATGTCAGACTTCGACAGAAAATTCTTT2340.3463485391196235No Hit
GGATATACTGCATGAGCATTTAACTTTTTAACAATCTCATTGACATCAAAG2320.3433882951955241No Hit
TCATAATCCCCACTATGACATCACAATCCACAATATGACATCACAACTTTC2220.32858707557502737No Hit
ATTGCTAGTAATACTGGTGAAACCCGTAGTTGATTGTAAAGGTTGAGATAG2160.31970634380272933No Hit
ATTGTGAACCATTAACATTCCTCATCATACTGTTTCATTTCTTTCCCCCTT2130.31526597791658034No Hit
GTTTTTTCTCATTTGCAGTAAACTTGTTGAATAGTTCTTTACGATAAACCT2100.3108256120304313No Hit
TCCATAATCTTCGGTAAGAGATTCATGAATGTTCTTCATGATAACTGAAGT2100.3108256120304313No Hit
ATACGAGCATTTGATATTTGATGATTTAGCGCATCAAAGTATATTACGCTA2030.3004647582960836No Hit
TTCCCAAACTTTTGATGATATACGCAGGAAATTAACGGAGATAGATATCAA2020.29898463633403394No Hit
GCTTACTAATGGCACAAACAAATTTTGATGTTAAAGAGCATAATGCAAAAG1990.2945442704478849No Hit
GGTAACTACTATTAGTTTGTTCCGCGGGAAAGAAACAGTTACATTTTTGAG1990.2945442704478849No Hit
ATCAAGAGTAGTGTTTATGTTGCTTAAGGCTTGGATAGTTACAATTTGTGT1980.29306414848583523No Hit
ATGTAGACGATAGGGTTGGTAGTATCAAAGAAGGTAAAGACGCAGATTTAG1950.28862378259968624No Hit
CAATACAAAAACTCGTGTTTAGCAAATGGTTTTCTGACTTCCAACTTTATA1930.28566353867558686No Hit
GTATGCTTGAATTTCAACTTGTCCATCTTCATCTTCTTCTGCATTAACTGG1870.2767828069032888No Hit
ACTTACTATCATGAAAGATAGTAGATTAGGAACCTATGGCGTAATTGGCCT1820.26938219709304045No Hit
ATTGCTTATAGACTACAAACCTGTACAGCATGTTACTGTACTGAATACCAT1810.2679020751309908No Hit
ATGTAGTTTCAAAACGTGGAGATTCTGTTGTAGTAGTAGGTATTGTTGTTG1780.26346170924484175No Hit
ATTAAAAGACATTCTTCATACAAGTCAGTTTTTAGCAGTTGTACAAATAGT1750.25902134335869276No Hit
ACATAGATTGGAATTAATGGTCACTTGAAACCGTGATGGTATGTCTAAAAA1750.25902134335869276No Hit
CATTCAATGAATCCATTCGATTCCATTCTATGACGATTCCATTCATTTCCA1720.25458097747254377No Hit
CTTTAGTACATATGAGACGAAATTTGCAAATAAGTTCTTTTATCCGTACAT1710.25310085551049405No Hit
GTAAAAAGAAAACTGTCATTGCTGAAGCAAATAAAAATACACCTTTGGGTG1680.24866048962434503No Hit
GTGTTTTGAAATTCTTGTTGTAAAGATTTTTCACCTCCCTGGTTAGCTGTA1650.24422012373819602No Hit
GAGTGATGTATGAAATTCTGAGACATTACTGAGAGGACTAGTAGAAATAGT1620.239779757852047No Hit
CAGTGTAAATATGAGCCATTTTAATGGTGATATGCAATTAACTTTTATCGG1580.23385927000384835No Hit
ATTGAATGTTACACTTCTCAATTGCTTTATCGCTTCCATTATCAAGAAGAA1510.22349841626950062No Hit
TTACTACCGGAATTGTACCATAATTCGGAATAATCTCCCGACTGTTTGAAA1470.2175779284213019No Hit
GTACTACTTTAAACATTCTGAATTTTTCTTGCTTTGGGGATTTGTTTTCTT1450.21461768449720256No Hit
ATGTAAAACTTCCTCTTGGAAAGCTATTCTGTCTTTGATTTTTCAGAATGT1420.21017731861105354No Hit
GTTTTGAGAGTTTGATGAGTAAGTAATGACAGATACAACAGCATCTTTAAC1410.20869719664900385No Hit
GTGGTAAGTACATTACTACTCATATCTATTGTCACATCGGATTGATGCAAA1410.20869719664900385No Hit
ATATGAAACTCTAGGCAAGCTCTGTATTTTTGTTTGTTAATGTTTGTTTCG1380.20425683076285486No Hit
ACTGTAGGGTATTATGCTGAGTGAAATAAGTCAATCGGAGAAGGACAAACA1370.2027767088008052No Hit
GAAATAACTAAAACCACTGGTTCTGATGGAATAATATCCTTTGATGGACTA1360.20129658683875554No Hit
GATCTTCCAGACAGAAAATCAAGACAAAAACATTGGACTTAATTTGCACTC1350.19981646487670585No Hit
ATCTAGACATCATGATTACAAAACAGATTGAGTGTAATAAATTGCTCATTA1330.1968562209526065No Hit
GGATAAATAACTACTGCAGTGTTAAAATGTGACTGTTGTAAAGTGAAGTAC1330.1968562209526065No Hit
ATGTTAAATGAAATAAGTCAGAAAGACAAACACCATATGATTTCACTCATA1310.19389597702850714No Hit
ACTTGAGTGTGTTAGAAATATAACCTTTGTTGGTGAATTCCTGAAGGAAAG1290.1909357331044078No Hit
CAAAAACAGTATGATCATGTCAACTGAAACTGAAAAAGCATTTGATGAAAT1290.1909357331044078No Hit
GAATGGAATCATCGTAAATGCAATCAAGTGGAATCATCGAATGGAATCTAA1270.18797548918030846No Hit
AATCAATACAGAAACAACAAAACAGTAGAATGGAATAATGAAACTAAGAAC1260.1864953672182588No Hit
GTATAAGATGAGTCGTACCATTTTTGTATTAATCCCTTGTCTTTTTCTTTT1260.1864953672182588No Hit
CAATGATATAAGTCAAACTGAAGATAAAACTCATTTCTCACTGAAAATTTA1250.18501524525620913No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG1250.18501524525620913No Hit
ATACAACAATCTTCCGTTTATTCGTTACATCAATCAATTCTGTTTCAACAA1250.18501524525620913No Hit
AGCTTATATTTTCACAGTGACTATTCACAAAACAAACAAGGGTTTCATATT1240.18353512329415944No Hit
TTAGTCCACTCATAATCCAAAAATCAGACATGCAAGAGATTTTAGAATACC1230.18205500133210978No Hit
AACAAACAAAAAACACTCATGAATACTCACAAATAGTTTCCAGATTCTGGA1220.1805748793700601No Hit
GTAATGGATGAATAGGCGATTTAATCCGTAATCATCTGATAGTGAACCACC1210.17909475740801042No Hit
TCCTAATGGTGTGAAATAGTATTTCACTGTGGTTTTTGTTTTTTAGTATTT1170.17317426955981174No Hit
GTTGTACAGTTGGTTGTACAGTTTCTGTAGGGGTAGTTGTACACTTACTCT1160.17169414759776205No Hit
GTTAAAGACACTTAAGAAAATGAAAAGATGAACCACAGATTGCAAGAAAAA1160.17169414759776205No Hit
GTAGGTAATTGATGTTGAAGAAACATTTTCTTGACCACTGAATAATGTAAG1110.16429353778751368No Hit
TATTTATGGAGTACAGGAAATGTTTTGATAAAGGCATGCAATGAATAACAA1110.16429353778751368No Hit
GCTATCTAACAGCTTGATTGCAATATCACATGGTGGATTATTTGGTACTGG1090.16133329386341433No Hit
CAAATCACCTGTAAAAAAGATGATATCTGGTTTCTGTTTCATCACCAATTC1080.15985317190136467No Hit
ATTTCATCTATGAATGTTCCGGTAATCAACATCTTATTCCATTTTTTTTAG1050.15541280601521565No Hit
CCTATAGTCTTCATATGATGTTTTCGTTCTTGAGAACAATTCCTGTGTGAG1040.15393268405316599No Hit
GATACTTTCAAAGGAACCAATGTTATGAGTATTGTACTTGAATTCCCTAAA1040.15393268405316599No Hit
ACTAATGGTATTCTGGTAAATTTTCAAAACCATAACCCTAAAAACGTGACA1030.1524525620911163No Hit
ATTTAGTTGTTATCATTTAAAATCCAACTTGAACACCGAACGTGTAATTAG1020.15097244012906663No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA960.1420917083567686No Hit
GTTAGTAAGTATTGGTCGGTAATTAAGTGTCACGTGATTAGAAAATATGGG940.13913146443266924No Hit
TACTAGCCCTTTATCTGATAGGTCATTTGCAAATATCTTCTCCCATTCTAT940.13913146443266924No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.13913146443266924No Hit
ATCTATTACATCACAAGACGAGATTTTTAAACCTTAATACCAGATTGCTAA930.13765134247061958No Hit
CAGTAAAATAGCGTAGATAACAGCAGAAGGTGTAACTGTGATTATGAAGCT930.13765134247061958No Hit
GACTCAACTAGATTATAACTTCCTAAAGAGCTATCTTCTTCTCTTTAATGT920.1361712205085699No Hit
ACTGCATGTTTATTGCAAAATGGTTGAACTTCTGGAAGTAAACATTTGCAG920.1361712205085699No Hit
ATCATTTACTTGCTCAATAAGTCTATTGAGAAATTTCAGCAAGAAACTGGC910.13469109854652023No Hit
ATAGTAAGTTGTATCAATTAAGTTGCTTTTGGTTACAGGTAGATCAATTCA910.13469109854652023No Hit
AACATGATGAAAGAAATTCAAAAAGAACGCGGACTTGCGTATCTATTTATC900.13321097658447056No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.13321097658447056No Hit
ATAATAATGTGGACTTTGACAAATCATTTAATCTCTCTGAGTCTGTTTCAT890.13173085462242087No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA880.1302507326603712No Hit
ATTTTATTCCATGATTTTACCTACTCCCCATCCTAAAATAAATACAATGCT880.1302507326603712No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.12877061069832155No Hit
GTAAAAACGATCAAAACGTTCACTATTCGCAAATGATCGTACAAAATTAAG870.12877061069832155No Hit
GATCAGTATGTAGAAATTGAATTGTTGTCACTGAAACAAGTTTTTAAAGAG870.12877061069832155No Hit
GATTTAATGTAGACATATTGGCATTTGAAAGTGCAAATACTGTCTTTATAA860.12729048873627188No Hit
GTAAAAGTATCAGAGGGCTTATCTGTAAATGCACCTTTATATTTCCCTATT860.12729048873627188No Hit
ATCTAAGGAAAGACATCGCTAATCAGAACAAAAAAAGGAAGAAGTAATAGA840.12433024481217252No Hit
TCAATAAAGTGCTTAAAAAAAGACAGACATACAAACCAATGGAACTAAATA840.12433024481217252No Hit
CTTCATAGAAATCCATGAGATAGGGGTTGGTATCTACATTTTCAAATTGTG830.12285012285012285No Hit
AACTTATACTCATAGCGGTCTAAATGATCTGGAGTGATGTTTAATAATACA820.12137000088807318No Hit
TCTTTATTTTCCGCTATAAGTAGCAATATTTTTTCGGCTACAACTCTTTGG820.12137000088807318No Hit
AAATAACACATGAACAAAGCCATTCATTTCCTCAAGTAAAAGATCAGAAGC820.12137000088807318No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.12137000088807318No Hit
ACTACGTACTGATACTAATAACAAGTTATCATCAGAGGAAAAGGCTTTTCC820.12137000088807318No Hit
TGTCAAGAAAGATTGCATCTATTGCTTGCCCTTGATTGATCAAGTTTAGAT820.12137000088807318No Hit
TGTTTGTACTGAAGCCAAGATTAATCGTAAAGTTTTTTACCAGTGTTGTAC810.1198898789260235No Hit
AGTTTATACATTGTGATGGAGGAATTATGAAAGTCTCTTTAGGATGAGGGT810.1198898789260235No Hit
CATGTGTAACTTTGACAAACATAAAAGCTAAATTACACAAGTGACCAAATG810.1198898789260235No Hit
GTTGTATTTATTTGTGTGAGGAATGTCAATGGTATTTTTACAGAAATTGCG810.1198898789260235No Hit
GCATATAGCAATGTTTGTATTTCTTCAATTGCTTTTTTGAATTGGTCAAAT800.11840975696397382No Hit
ATTTTATTGCTAGGTATTTTTGGCTCTTCTGGGTATTTAGTGGATTTAAAT780.1154495130398745No Hit
AAATTGGGAAAGGCTTGCATTAAATCTAATCTGGACAAGGGAAAACCAATG780.1154495130398745No Hit
GATGATGATGATTCGAATACGCATAGTCAAGAAATCGAAAATAACAAACAA780.1154495130398745No Hit
AAGTAAGCAGCAATCAATATGAACTTAGTAACAGATGGGATGAGAAATTAG780.1154495130398745No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA770.11396939107782482No Hit
GTTAACATACAGTGTGATGTTAGTTTCAGAAGCAGAATTTAGTGATCATCA770.11396939107782482No Hit
GTATAAGCATGGCAGAGGAAGAAGAAAAAAATAAGGAAGGTTCTTGAGTCC770.11396939107782482No Hit
GTTTGTAGACTTTGTTGATCTTTTCAAAGAGAAAACTTATTGATCTTTTTA760.11248926911577514No Hit
CACCTACCCTAATAGAGTATGTTTCACTGGTTTTAGCTACTTTATCTTCTT760.11248926911577514No Hit
AATCTATGCACCTCAATAAACATCGGGTTTAGTGGTACATCTTTTATGGCT750.11100914715372547No Hit
GATTATAGTCACCCGTTAAAGAAAGGTGCTAAATTGGAACTGGGTGTAAAA750.11100914715372547No Hit
AATAGTTGAATGAATTGAAAGTGTTTAGACTAGAAAAGGAGATGATCATCA740.10952902519167579No Hit
TGTTAATGCATTGTATTCCTTAATCGACATAATAACAACTGCATCATCCTC740.10952902519167579No Hit
ATGCTAATAAGAGCATAATGGTTGAATGAGTTTACAAGATAATTGTTCAGG740.10952902519167579No Hit
GGTAAGAACACTTAAAATCTATGAAATAGCCTCATTTTCTTTTGATTTTTG740.10952902519167579No Hit
ATATACATCAAAACAAATGCCATTTTTATGCATTTTTGTCCTTACATTGTT730.10804890322962611No Hit
ATTATGCTACTACTGAAAAAGAATTTTTAGCAGTTGTATTTGCTTGTGATA730.10804890322962611No Hit
GATTTAATCTAAGAGGGTTGTATGTTTCCAGGAATTTATCCATTTTCTCTA730.10804890322962611No Hit
AGATAAACGTATTCACGATCTTAAATTATCTCGACAAATTACGTTACAATC720.10656878126757643No Hit
GGAAGAAGGGATTATCAAAATAACCATTTTCAGATTATGTCATTTCAATAA720.10656878126757643No Hit
CTTAAGTAGAAAGAGATGAAATGATGAAGAAGAAAAGGAGCTTCAATAAAA710.10508865930552677No Hit
CTTATAATGTGAGTCGTTTATTCGCGAAATTTTATGAACAACAATTGAAAC710.10508865930552677No Hit
ATATTACAACCAGGATATTGACATTAATACAGTCAAGATAGAGAACACCAC710.10508865930552677No Hit
CATCTTAACTTATAAGAACCAGCTTCAGATTTTTCCTAGCTTAATTTCAGT710.10508865930552677No Hit
ATATAAAAGAGCTAGGTAAAGGAACACAACAAATATTCCTCCTATAAATCC700.10360853734347711No Hit
AAACTTATCTACTTTCAATTGTTCGAAAGCAAAATCGTAAATTTTCTTCTT690.10212841538142743No Hit
AGTATGAGTATCAATAATGAGATGATGATTGACAAGGAAGATGAAGAAGTA690.10212841538142743No Hit
CATTACTTGTAATCGTAATTGTCTTGCCACTTGTAATGATAAAATTTTCTA690.10212841538142743No Hit
TCATATAGGTAGACCATAATTTAGGCAACTATTTCCTTGATAAACTGTGAG680.10064829341937777No Hit
CATTAAAAAGTGGGCAAAGGACATGAACAGACATTTTTTAAAAGAAGACAT680.10064829341937777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAGC302.1351989E-645.00000418
ACTATCC302.1351989E-645.00000437
ATCTCAT302.1351989E-645.00000434
GATAAGG302.1351989E-645.00000426
CTCAAAC302.1351989E-645.00000411
GAGTCTT302.1351989E-645.00000432
CTGAGAT302.1351989E-645.00000417
GCTATGA302.1351989E-645.0000046
ATAGCTA302.1351989E-645.0000043
ACTTCGA302.1351989E-645.00000432
ATCACTA302.1351989E-645.00000430
TAGCCTA302.1351989E-645.0000044
TCGGCTA302.1351989E-645.00000434
ATGTAGC302.1351989E-645.0000041
TAGCAGG302.1351989E-645.00000424
GCTCTGT302.1351989E-645.00000419
TTCCCCA302.1351989E-645.00000420
GTAGCCT302.1351989E-645.0000043
CACTAAC302.1351989E-645.00000432
AGGCAAG302.1351989E-645.00000413
Produced by FastQC (version 0.11.2)