H23LNBCXX_l02n01_bir_1228_pcontrolb_78.3510000004350d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_78.3510000004350d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334096
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	11121	3.3286839710741827	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT	1182	0.353790527273598	No Hit
GTGTTAAGATTGTTCGATCTGAGAAGGTTAAGGATGAGATTATTCTTGAGG	904	0.2705809108759159	No Hit
ATATTGGATGATTCCATTCGATTCCATTTGATGTTGATTCAATTCGATTCT	879	0.26309803170346246	No Hit
ATCATATCAAGTATCTTTTCTGACCACAATGGTATAAAACTGGAAATCAAT	550	0.16462334179397536	No Hit
GACAAAAACATTGTACCATAATGGGAGTTTCAAAGAAATACCACAAGCTAA	528	0.15803840812221637	No Hit
TTCTTGTAATTTTTCACGATCGTAATCTGAAGTAGTATTTTCAACTTGTGC	507	0.1517527896173555	No Hit
ACAAATACATTTGGTGACCACTCTGTGTTACTCCTTTGTCTTTCTTTGGTT	502	0.1502562137828648	No Hit
ATGTTGTTGGTACTCTGTTTTCTTTCTATGGATTTGATCTTGGTTTAAGTT	484	0.14486854077869835	No Hit
GATTACAGATTTGGTAGAAACTAGTTTAACTCAATGAGCAATAACATCACT	483	0.1445692256118002	No Hit
GTTTAGTGATTGTTGATTGTCACATCAAACAACTTACAAAAGGATCACTTT	474	0.14187538910971695	No Hit
TTATCATCATGTCTCATATGAGAAGTTTCATGTGATGTGTAGCTTCTCCTT	470	0.1406781284421244	No Hit
GTGTATCAACTATATCTGTTGTTTTATGTCAACTTTTAGCTAGCTTTTAGG	463	0.13858292227383745	No Hit
ATGAAATAGAATGCAATCATCAAATGGAATCGAATGGAATCATCATCGAAT	450	0.1346918251041617	No Hit
ACTTGTTCGAAAAGAAACAAAAGAGAATGCTTGGGGAGATAGAAGTGTGAG	439	0.13139935826828217	No Hit
ACTTGTTCAAAAAGAAACAAAAGAGAATGCTTGGGGAGATAGAAGTGTGAG	432	0.1293041520999952	No Hit
TACTGATTGTGTCCAACTAAAAACTTAAAACAACACCAGAGATAACGATAA	429	0.1284062065993008	No Hit
AAACCATACAGTTTTTGGACCTATGTTTGGCATTTTAACCAAGCTATTTGT	427	0.12780757626550454	No Hit
ACTGAAGAGTATCAGAACTGAAGTATCCTTCTCATTCCGGTGAAGAAGAAT	422	0.12631100043101384	No Hit
CTTTAAAGGCTTTTTTTCATTGTTCGCATACAAAAAGTGAAACAGAATAGC	422	0.12631100043101384	No Hit
AACATTGCTTTATGGTCAGAATCAAAGAACTCAAAACTTGAAACAAACTCT	419	0.12541305493031943	No Hit
TATGATTTCAAAGTGGATTATAGGAAGAACAAAGATGGAGAAGACGAAGAA	413	0.1236171639289306	No Hit
GAACTGAATCAAGAGAGTTTCCTTCATCCTAAATACCACAAAACATAAACA	410	0.12271921842823619	No Hit
AGTCTAATCTGAGGAGAAACAAAAATCAAATTGACAAGAAAATTGACACAA	402	0.12032469709305109	No Hit
ACAGACATATAATCAGTTTTGTTACAGGACAAAAAGATTCATCTAGTACCA	400	0.11972606675925482	No Hit
GTGGAAGAACAGATAATGACCATTTATACCGGAACAAATGGTTATCTGGAT	398	0.11912743642545855	No Hit
ACTAACAGCCATTAACTAAATGAGCAGAATAAACACCTGAATTAAGATCTG	397	0.1188281212585604	No Hit
AAGTTTAACATAGTAAAGCTGCAAGTGTAATAACCACACGATGAATCCAGT	395	0.11822949092476413	No Hit
AAGAGAAACTAACATTTTCAATTCAATGACAAAGCCACATAGCTAATAAAT	393	0.11763086059096786	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG	390	0.11673291509027345	No Hit
AAATACAGCTCAACCAGAAACACCAGTTGAAAACAACATTACTGATAAAGA	380	0.11373976342129208	No Hit
TTTTCTACCTTCTAGAAAGAGGTCAAAAAGTTAATAGCTTCACCGTGAGAA	380	0.11373976342129208	No Hit
ATTCCATTCCATTCCATTCCATTCCATACCATTCCATTCCATTCCATTCTA	378	0.1131411330874958	No Hit
ATATTGACGATGATTATCAAATCGTTGCAAATACGATATCAGATCCTACAC	376	0.11254250275369952	No Hit
GTTTGAAGTAAAGTCAACAAATGGAGATACTCTTCTTGGAGGTGAAGATTT	374	0.11194387241990328	No Hit
ATTCAAATGCTTTTAAAACCAACCATGCCTATCAATCAAGTGTGTATAAAC	370	0.11074661175231072	No Hit
TAAGATACCTAGGAATAAACCTAACCAAAGATGTAAAGGATCTATACCCTC	367	0.10984866625161631	No Hit
ACACAATGGTATAGTGAAAGTTGTGACTTGGAACAATCATTATCAGAAGCT	367	0.10984866625161631	No Hit
GTTATGTTCTTTGCGAAAATAAAAGTTATTCCACAAACGCATTCGATAAAA	362	0.10835209041712562	No Hit
TAGTTATACCAATCCTCAAGATGTGATGAACGTTGTAATCGATCAGATTCC	362	0.10835209041712562	No Hit
TTACACCTATTACCTATTGTCTATCGTCTATCGTCTATTGTCTATTGTCTA	356	0.1065561994157368	No Hit
AAGAAGAAGTCTAAGAAGTGAAGAGGATATATAGTGTGAAGCTATATCGAT	353	0.10565825391504238	No Hit
GATACAAGCACATTCATTATGTTTGGTATGACATGATCAGAGTGAATAATA	350	0.10476030841434797	No Hit
GCATAATTGTATAGTCTCCAAAATTTGCAAAAACAAGTCATCCTGAGAGAG	349	0.10446099324744984	No Hit
GCTTAAATGAGTGCATATACTCATTGGAAAAGAAACAAAGCTTTCCTCTTT	348	0.1041616780805517	No Hit
GTTTCCATCATTGTGATTCAGATATTCACAGAGCATACCAGTTTTGTTTCT	348	0.1041616780805517	No Hit
TGATTATGACCGTGATAAGAAGTTTTCAAAGTCCTTTAAAGAATTGGTAGC	347	0.10386236291365357	No Hit
ACCATAAGGATTTTCCAAAAGAGAGAGTACTTACTCTAACACAGTGGAAAA	346	0.10356304774675543	No Hit
GGGATACATGATTGATTAACCATGGTTAGGTTAACCGAAGATGAACCAAAG	345	0.10326373257985728	No Hit
GAATAATGTAACTCCAAGCATATTTGCAAACACAGCTAATTGAACGTCAGT	341	0.10206647191226474	No Hit
CATTTATGCATGGTAACTTCTCTCCTAATGAAGTTTAGCAACTAAGATTGT	338	0.10116852641157033	No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT	338	0.10116852641157033	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTT	20	7.0260937E-4	45.000004	20
GGTACGT	20	7.0260937E-4	45.000004	44
CGTATTG	20	7.0260937E-4	45.000004	11
CGAGTTA	40	6.7902874E-9	45.000004	20
GCGAAGC	20	7.0260937E-4	45.000004	29
TGGACAC	70	0.0	45.000004	33
GACGTAA	40	6.7902874E-9	45.000004	6
GCAGTAT	30	2.1608612E-6	45.000004	35
GAATCGG	20	7.0260937E-4	45.000004	17
CGACCCA	20	7.0260937E-4	45.000004	11
GTTAGTG	20	7.0260937E-4	45.000004	41
CCTCGTT	20	7.0260937E-4	45.000004	34
GTCAGAT	20	7.0260937E-4	45.000004	2
CGAGGGA	20	7.0260937E-4	45.000004	35
AACGCAC	20	7.0260937E-4	45.000004	36
GGTATGC	20	7.0260937E-4	45.000004	42
GCTAGCT	35	1.2084638E-7	45.000004	39
GGGTTGC	20	7.0260937E-4	45.000004	30
GACGATC	20	7.0260937E-4	45.000004	30
AGCTAGC	35	1.2084638E-7	45.000004	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content