FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_77.351000000434d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_77.351000000434d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214824
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC140446.537444605816854No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT78273.643447659479388No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG17110.7964659442147991No Hit
GGTATAACACAGGAACGGAAACATAGTAGAACACTTATTCATCAGGGATTA3580.16664804677317246No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3470.16152757606226492No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3400.1582690947007783No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT2510.11683983167616281No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2300.10706438759170298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAC302.15424E-645.009081
TGCCGTC15450.044.12484445
GCCTTAT356.2159743E-638.5792121
CGAGTTG356.2259223E-638.57023240
TTAGACT301.1354842E-437.5075654
CTACCAT301.1354842E-437.5075656
AGTGGGG301.1370421E-437.49883731
AGCTCCC301.1370421E-437.49883737
GCCGTCT301.1370421E-437.49883745
TTTACCG250.00210122236.0072635
AATGCGT250.00210122236.0072636
GCTACGA250.00210362535.99888212
TAAGACG250.00210362535.99888210
AAAGCAC250.00210362535.99888221
GATCGAG401.5550195E-533.7489537
ACGCAAG401.5550195E-533.7489517
AATCCCT501.968614E-631.5063555
GTTTATC453.4729914E-530.0060541
ATGGGTG300.005132985830.0060526
ATGGGAC300.005132985830.0060526