FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_76.351000000434bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_76.351000000434bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315839
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC80642.5531995732002697No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT21620.6845259768426318No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT12610.3992540503231077No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG4000.12664680422620384No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCCTGTCTCTTAT3960.12538033618394182No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT207.0109026E-445.019014
TATACGC207.0109026E-445.019014
GGAGGTA207.0109026E-445.019012
GCGAGAT207.027335E-444.99762742
TCCGCTC207.027335E-444.9976278
GTCGAGG207.027335E-444.99762736
CGCGGTT207.027335E-444.99762729
GTCCTAG207.027335E-444.99762730
AGTGCTC207.027335E-444.99762740
TACGCGG207.027335E-444.99762727
TCAGCGC207.027335E-444.99762737
CCGTTAG207.027335E-444.99762728
CTAGCTG207.027335E-444.99762739
GCACGGT253.8855702E-544.99762327
CGCAGAT253.8855702E-544.99762324
AAGGGGG253.8855702E-544.99762325
TGCCGTC7950.044.71462245
AGCCCTC1900.043.8134816
CAGCCCT2100.039.64076615
GCCCTCA2100.039.64076617