FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_75.35100000043492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_75.35100000043492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232697
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC81783.5144415269642493No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT8790.3777444487896277No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3340.14353429567205422No Hit
GATAAGAACTTACTACAAGACCATACAACGTGGTTTTGGTATCTGTCCATA2770.11903892185975753No Hit
ACTTAGTATCCGTTTATTCAACACACATCTAGGTGTATTCCGTAGTTACAT2760.11860917845954182No Hit
ATATAGTCAATGGTACTGTAATAGCACTGAATGGTGACAGATAGTATCTAC2460.10571687645306987No Hit
TGTAATATTGCTTCCAAAGTATTTCCGAATACCTATTTCATCAATTCCCTG2450.10528713305285413No Hit
AATTCATTCTTCAAGTTTCTCCATTCTGATAGTTTTCAATCTGACACTATT2390.10270867265155974No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCC207.0153986E-445.008173
CTAACGT253.877026E-545.008174
TCTAACG253.877026E-545.008173
ACGAGAT253.877026E-545.008173
GGGTGGG207.022834E-444.99849724
AATCCGT207.022834E-444.99849725
CGAACTA207.022834E-444.99849738
CAACGTG351.206663E-744.99849726
CCGATTC207.022834E-444.9984978
GGCGTAT207.022834E-444.99849715
GGACTCC207.022834E-444.99849710
GGATCCA207.022834E-444.99849745
GCACGAG207.022834E-444.99849733
ATTCGGT207.022834E-444.9984978
ATTCGGA207.022834E-444.99849716
TAACGTG207.022834E-444.99849720
GTGGCTC207.022834E-444.99849710
CCATGAC406.7775545E-944.99849722
ATCGTCT207.022834E-444.9984978
GACCAAC207.022834E-444.99849720
Produced by FastQC (version 0.11.2)