FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_72.35100000043435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_72.35100000043435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8301
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC5686.842549090471027No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT770.9275990844476569No Hit
ATATTGCAACTGATTATTACCACCATAGCATTAGCTAATACCTCCATCACA240.28912179255511383No Hit
ATTGGTAATGTAGTCAATACTGATGTTGTTCATAGGAAAAGAAGTAACTTC240.28912179255511383No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC180.21684134441633537No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG180.21684134441633537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.19274786170340924No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA160.19274786170340924No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.18070112034694613No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA150.18070112034694613No Hit
ATCTTACACGTATGGTTACTATGCAAAATTTATTTCATGATGGAGGTTTTT140.16865437899048308No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.16865437899048308No Hit
ATACACAACTAAACTTAAACCTAAAGGAACTGGAGAAAGAATAGCAAATAA130.15660763763402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.15660763763402No Hit
GAAAGAGAAATTAAGGAGTCAATCCCATTTACAATTGCACCCAAAAGCATA130.15660763763402No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA120.14456089627755692No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA120.14456089627755692No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC120.14456089627755692No Hit
ATATATCCATTTGCAGATTCTACAAAAAGAGTGTTTCAAAACTGCTCAATC120.14456089627755692No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC110.13251415492109384No Hit
AACTTAGTTTTATCATTCGTTTTTAAAGCGTAATCCTTATAAAACTTACTT110.13251415492109384No Hit
TAATTTTATTTTGCGTTTTAACGTATTTCTCAAGCCATTGATATTGCTCCC100.12046741356463078No Hit
CTATACCAATGAGCAATAAGCAAAAAAATCTTATCCGTAACATATTCACTT100.12046741356463078No Hit
AAGTTGACAAAATTTGGAATAGACTTGATTAAGAAAGTAAGAGAGAAGGCT100.12046741356463078No Hit
ACATCAAAAGGAATAGGAGTTAGTAACATGTATAAGAGGGCATCATTTATT90.10842067220816769No Hit
AAATTACATGATGACTGAGATTTGCTTTAAAAGACTCTAGCAAAAAAAAAA90.10842067220816769No Hit
TAGATATCCATTTGTTCCAAAGCCATCTACTGAAAAGATTATTTTCCCCCA90.10842067220816769No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10842067220816769No Hit
GAATTAGATTGGTATAAAGAGTATTGGATCAATAGTACCAAAACCATTGAT90.10842067220816769No Hit
CCATAGATCATCATAGGGCAAATAAGAAATATGATTCTCATGTTGAGCTTA90.10842067220816769No Hit
AATTTATACAACCTTATTCATGCACACACTAACTAAAACAGCAACAAAGAA90.10842067220816769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC900.040.045
AGCCCAC1000.038.2517
GCCCACG1000.038.2518
CCACGAG1000.038.2520
GAGCCCA1000.038.2516
CACGAGA1000.038.2521
CCCACGA1000.038.2519
ACGAGAC1000.038.2522
CGAGCCC1000.038.2515
CCGAGCC1000.038.2514
CGAGACG1000.038.2523
GAGACGA1000.038.2524
GACGCTA950.037.89473729
AGACGAC950.037.89473725
GACGACG950.037.89473726
CTATATC900.037.533
ACGCTAT900.037.530
CGCTATA900.037.531
TATCTCG850.037.05882336
ATATCTC850.037.05882335
Produced by FastQC (version 0.11.2)