FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_71.3510000004341b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_71.3510000004341b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113036
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC93468.268162355355816No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT39163.464383028415726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG9630.8519409745567783No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2220.19639760784174953No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1510.13358576028875754No Hit
GTTATGGTTCATTTGATCGTATGGTTTACTTGGATAACTGTGGTAATTCTG1250.11058423864963374No Hit
GTACCAACATTACGAATTGATGTTATACTAACGCTGTCTCTTATACACATC1230.10881489083123962No Hit
ATTACACAGTCTCAGGTAGTATCTTTATAGCAGCATGAGAATGGACTAATA1180.10439152128525425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT207.006287E-445.014
GGTGTGA207.006287E-445.042
CGTATGG207.006287E-445.018
TTTACGC207.006287E-445.022
GCGAATA253.8684433E-545.026
TTAGCGA253.8684433E-545.023
TAGCGAA253.8684433E-545.024
ATTCGAC207.006287E-445.019
TTTCCGT253.8684433E-545.011
TGTGCTC207.006287E-445.010
TAACGAG207.006287E-445.041
GGCCATT207.006287E-445.011
TACGCTA207.006287E-445.024
AGTGACT207.006287E-445.08
CGTAGCA207.006287E-445.042
GGGAACC207.006287E-445.030
CGCTAAA207.006287E-445.026
GTGTCAG207.006287E-445.031
CACAGTC207.006287E-445.05
AGCGAAT253.8684433E-545.025