FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_70.351000000433f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_70.351000000433f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100320
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC51115.09469696969697No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT19061.8999202551834131No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG4140.4126794258373206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5100.044.11520845
GATCGTG250.00209854235.9980057
ACCATAA250.00209854235.99800513
TCGGATA250.00209854235.99800530
TATAGCA401.5417725E-533.764964
ATGCCGT7250.031.03276344
TATATAG300.005114041330.0133022
CTAACAT300.005114041330.0133023
GTTAACT300.005114041330.0133021
TTGTCAT300.00512653729.99833920
GATTTGT300.00512653729.99833917
ATCGTGT300.00512653729.9983398
CGGATAA300.00512653729.99833931
CTCGGAT300.00512653729.99833929
ACTCCTG300.00512653729.99833934
AAGGCTG300.00512653729.99833939
AATACGA300.00512653729.99833915
CGACGCT7750.029.61126128
CGAGACG7750.029.61126123
GACGACG7750.029.61126126
Produced by FastQC (version 0.11.2)