FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_69.351000000433d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_69.351000000433d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63026
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC2260235.861390537238606No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1289220.455050296702947No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG27494.361692000126931No Hit
ATAGGATAGCTTAGCACATTCCATGTTTTCGATTGATAGTAATTCGTGTTT2630.4172881033224384No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1570.24910354456890807No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGT1400.22213054929711545No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC990.1570780312886745No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA980.15549138450798083No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA840.13327832957826927No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCC790.1253450956748009No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA206.98258E-445.041
CCAATTA206.98258E-445.028
TAGGATA351.18765456E-745.02
GCCTCGA206.98258E-445.039
TCGATTG351.18765456E-745.029
AGCTTAG351.18765456E-745.08
TACAGGG253.8488368E-545.033
TTCGTGT351.18765456E-745.043
AGGATTT206.98258E-445.07
AATTCGT351.18765456E-745.041
AGGATAG351.18765456E-745.03
GGACTCA206.98258E-445.019
CCTCGAA206.98258E-445.040
TTCGATT351.18765456E-745.028
TAGCTTA351.18765456E-745.07
ATAGGAT351.18765456E-745.01
TTTTCGA351.18765456E-745.026
TTACAGG253.8488368E-545.032
AGTGGAC206.98258E-445.016
AGGGCCT206.98258E-445.036
Produced by FastQC (version 0.11.2)