FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_68.351000000433bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_68.351000000433bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68248
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC34415.041905989919118No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT13231.9385183448599228No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG2780.40733794396905404No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT2700.395615988746923No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC760.11135857461024498No Hit
TTTGTGTATACTGTTGGACTGTTGTAAGTAGCTGTTGTATGTCTGTCTCTT700.10256710819364671No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTC206.987221E-444.9992684
TACGTAA206.987221E-444.99926821
TGCCGTC4050.043.88817645
CTCAGCC356.162989E-638.570813
CAGCCCT301.1288097E-437.49938615
TCAGCCC301.1288097E-437.49938614
GTACTAA250.002085904836.0258261
GCTGATA250.002093437635.9994168
TTGGTTC250.002093437635.99941617
ACAGACC250.002093437635.9994168
TATACTA250.002093437635.9994165
TTATGTC352.793097E-432.14233435
CCTGTTA352.793097E-432.14233431
GCCCTCA352.793097E-432.14233417
AGTCTCA352.793097E-432.14233410
AAGTCTC352.793097E-432.1423349
ATGCCGT5500.031.90857144
CTACTTA300.005095847430.0215231
TATCAGG300.00511418129.9995133
GTGTGAT300.00511418129.9995121
Produced by FastQC (version 0.11.2)