FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_67.35100000043393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_67.35100000043393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135334
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC63194.669188821729942No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT11240.830537780602066No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG3530.26083615351648515No Hit
TAAGAGAATCATTCATTACGTGTACTTACAACAATCTGTCTCTTATACACA2290.16921098910842805No Hit
TAACAGTTTCATCAATAGAAACGTCTCTTGAGTTATCGTATGAATTGTACA1740.12857079521775755No Hit
CATATGAATCACACAATAGTTACAAGACTGTCATATATGATGACATACACC1680.1241373195205935No Hit
ATGTTAAAGAGATTCATAAACGAACAATGACAATTGTTGTATTTTGGGTTA1500.11083689242910133No Hit
CATCAAAGTTATCCAAATATCGATGATTTCCATACCTTTTTGCTAAGAAAT1400.10344776626716125No Hit
ATACTTAACTCCCATTTCTATATTCCCTGAACTTCACATTTGCTCTAACTT1380.10196994103477323No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAC207.000717E-445.013683
CAAGTAG207.000717E-445.013685
CCTAGAG207.000717E-445.013683
GGAGTAT253.8655722E-545.013686
ATGCAGT207.000717E-445.013686
ATACCGT253.8655722E-545.013686
TGCACCG207.000717E-445.013685
CTAGATG302.152252E-644.99704711
AGTCCAT207.013485E-444.99704710
GCTGGTG207.013485E-444.99704725
CCGATGT207.013485E-444.9970479
GGCACGA302.152252E-644.99704739
GACGTTC207.013485E-444.99704731
AGCTTCG207.013485E-444.99704730
AGTGGTC207.013485E-444.99704735
TTATCGT207.013485E-444.99704733
CTGACAG207.013485E-444.99704734
CTTGGTC207.013485E-444.99704724
CGTTATC207.013485E-444.99704718
AAAGTCG207.013485E-444.99704711
Produced by FastQC (version 0.11.2)