FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_66.35100000043379.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_66.35100000043379.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78600
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC3117639.664122137404576No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1731122.02417302798982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG34694.413486005089059No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1470.18702290076335878No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC970.12340966921119594No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT940.11959287531806617Illumina PCR Primer Index 11 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA830.10559796437659033No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA830.10559796437659033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT700.045.045
TGCCGTC32600.043.96472545
ATGCCGT50300.028.49403444
TCTTATA29800.026.728191
TATGCCG55100.025.9709643
TATATCT55050.025.95367834
GTATGCC55150.025.94741642
TCTCGTA55100.025.93012638
ATATCTC54950.025.91901835
CGTATGC55350.025.89430841
ATCTCGT55050.025.87193537
TATCTCG55050.025.87193536
CGAGACG55750.025.86995523
CTCGTAT55150.025.86582239
ACGCTAT55500.025.86486630
GACGCTA55500.025.86486629
AGACGAC55850.025.8639225
TCGTATG55350.025.85365940
GAGACGA55800.025.84677524
CGACGCT55550.025.84158728
Produced by FastQC (version 0.11.2)